可降解纳米氧化钇空心球载药体系的体外抗肿瘤效应

doi:10.3969/j.issn.1000-1565.2019.04.006

河北大学学报(自然科学版) ›› 2019, Vol. 39 ›› Issue (4): 366-371.DOI: 10.3969/j.issn.1000-1565.2019.04.006

可降解纳米氧化钇空心球载药体系的体外抗肿瘤效应

韩宇,张芳波,于林康,贾光,葛昆

收稿日期:2019-02-25 出版日期:2019-07-25 发布日期:2019-07-25
通讯作者: 葛昆(1981—),女,河北邯郸人,河北大学副教授,博士,主要从事纳米医学方向研究. E-mail: kaqikun@163.com
作者简介:韩宇(1990—),男,河北沧州人,河北大学在读博士,主要从事纳米医学方向研究. E-mail: hanyuhbu@163.com
基金资助:
国家自然科学基金资助项目(21807023);河北省创新项目(CXZZSS2018156);河北大学创新项目(hbu2019ss006)

anticancer effect of degradable nano-yttrium oxide hollow sphere drug delivery system

HAN Yu, ZHANG Fangbo, YU Linkang, JIA Guang, GE Kun

Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China

Received:2019-02-25 Online:2019-07-25 Published:2019-07-25

摘要/Abstract

摘要： 纳米氧化钇空心球在生物医学领域具有广泛的应用.以酚醛树脂微球为模板,合成出了尺寸均一、分散性好的纳米Y₂O₃空心球(HYNPs).它不仅在体外表现出显著的酸性降解行为,而且负载抗肿瘤药物阿霉素(DOX)后,载药体系HYNPs-DOX也表现出明显的pH响应药物释放特点.pH为5.0时,72 h的药物释放可达到70.46%;而pH为 7.4时,72 h的药物释放仅25.04%.进一步通过激光共聚焦显微镜监测载药体系在细胞内的DOX释放,发现随着时间的延长,细胞内DOX的荧光逐渐增加,表明DOX在细胞内释放量的增加.体外抗肿瘤细胞毒性结果显示,HYNPs对肿瘤细胞MCF-7和MDA-MB-231的活性没有影响,而HYNPs-DOX则表现出较高的体外抗肿瘤效应,与游离DOX相当.可见,该材料作为抗肿瘤药物载体具有潜在的应用价值.

关键词: 氧化钇纳米颗粒, 中空球形, 载药, 释药, 抗肿瘤

Abstract: Nano-yttrium oxide hollow sphere has broad application in the field of biological medicine.The uniform and well-dispersed Y₂O₃ hollow spheres(HYNPs)have been fabricated using phenolic resin spheres as template.The HYNP snot only exhibited a high DOX loading and release efficiency, but also showed the pH-sensitive degradableand drug release profiles. And the DOX release from the HYNPs-DOX in the cells increased with time. Moreover, the low cytotoxicity of HYNPs was found on MCF-7and MDA-MB-231 cells. When loaded with DOX, HYNPs-DOX exhibited a good anticancer efficiency in vitro, which showed a great potential in cancer treatment.

Key words: Y₂O₃ nanoparticle, hollow sphere, drug loading, drug release, anticancer

中图分类号:

韩宇,张芳波,于林康,贾光,葛昆. 可降解纳米氧化钇空心球载药体系的体外抗肿瘤效应[J]. 河北大学学报(自然科学版), 2019, 39(4): 366-371.

HAN Yu, ZHANG Fangbo, YU Linkang, JIA Guang, GE Kun. anticancer effect of degradable nano-yttrium oxide hollow sphere drug delivery system[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(4): 366-371.

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可降解纳米氧化钇空心球载药体系的体外抗肿瘤效应

anticancer effect of degradable nano-yttrium oxide hollow sphere drug delivery system

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