黄腐酸对大豆内源激素的调控及结瘤能力的影响

doi:10.3969/j.issn.1000-1565.2021.06.011

摘要/Abstract

摘要： 为探讨黄腐酸提高大豆结瘤能力的作用机理,以辽宁慢生根瘤菌(Bradyrhizobium liaoningense)CCBAU 05525和冀豆17为材料,采用高效液相色谱法(HPLC)探究黄腐酸对大豆结瘤初期内源激素含量、分布及结瘤的影响.结果表明:黄腐酸质量浓度为500 mg/L时大豆结瘤数量最多,比对照组显著提高73.1%,同时该条件下激素含量和分布变化最为明显,具体表现为大豆根、茎、叶中玉米素(ZT)、生长素(IAA)、赤霉素(GA3)含量增加,根中脱落酸(ABA)含量减少,叶中ABA含量增加.而黄腐酸质量浓度为1 000 mg/L时则表现为抑制大豆结瘤. 上述结果为解析黄腐酸提高大豆结瘤固氮的机理、增加大豆产量提供了理论依据.

关键词: 黄腐酸, 大豆, 高效液相色谱, 植物激素, 结瘤数目

Abstract: In order to explore the mechanism of fulvic acid improving soybean nodulation ability, Bradyrhizobium liaoningense CCBAU 05525 and Jidou 17 were used to evaluate the effects of fulvic acid on the content and distribution of endogenous hormones by HPLC and on nodule number at the early stage of soybean nodulation. The results showed that when the concentration of fulvic acid was 500 mg/L, the number of soybean nodules was increased significantly by 73.1% at most, and the change of hormone content and distribution was the most obvious. The specific performance was that the content of zeatin(ZT), auxin(IAA)and gibberellin(GA3)in root, stem and leaf increased, the content of abscisic acid(ABA)in root decreased, and the content of ABA in leaf increased. Fulvic acid at 1 000 mg/L inhibited soybean nodulation. These results provide a theoretical basis for analyzing the mechanism of fulvic acid improving nodulation and nitrogen fixation of soybean and increasing soybean yield.

Key words: fulvic acid, soybean, high performance liquid chromatography, plant hormones, nodule number

中图分类号:

Q946.885

侯慧云,徐梦蕾,郭昱娴,袁红莉,高同国,朱宝成. 黄腐酸对大豆内源激素的调控及结瘤能力的影响[J]. 河北大学学报(自然科学版), 2021, 41(6): 709-719.

HOU Huiyun, XU Menglei, GUO Yuxian, YUAN Hongli, GAO Tongguo, ZHU Baocheng. Effects of fulvic acid on regulation of endogenous hormones and nodulation ability of soybean[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(6): 709-719.

参考文献

[1] 王鹤,吕艳秋,丁亦男,等.低氮胁迫对不同品种野大豆幼苗生长特性的影响[J].长春师范大学学报,2019,38(10):99-103.
[2] LIU H, ZHANG C,YANG J, et al. Hormone modulation of legume-rhizobial symbiosis[J]. Journal of Integrative Plant Biology, 2018,60(8):632-648. DOI:10.1111/jipb.12653.
[3] BENJAMINS R, SCHERES B. Auxin: the looping star in plant development [J]. Annual Review of Plant Biology, 2008, 59(1): 443-465. DOI:10.1146/annurev.arplant.58.032806.103805.
[4] TURNER M, NIZAMPATNAM N R, BARON M, et al. Ectopic expression of miR160 results in auxin hypersensitivity, cytokinin hypersensitivity, and inhibition of symbiotic nodule development in soybean.[J]. Plant Physiology, 2013, 162(4):2042-2055. DOI:10.1104/pp.113.220699.
[5] ROY S, ROBSON F, LILLEY J, et al. MtLAX2, a functional homologue of the arabidopsis auxin influx transporter AUX1, is required for nodule organogenesis [J]. Plant Physiology,2017,174(1):326-338. DOI:10.1111/j.1365-313X.2010.04447.x.
[6] JULIE P, ANTON W, FEDERICO A, et al. MtCRE1-dependent cytokinin signaling integrates bacterial and plant cues to coordinate symbiotic nodule organogenesis in Medicago truncatula[J]. The Plant Journal,2011,65(4):622-633. DOI:10.1111/j.1365-313X.2010.04447.x.
[7] 王龙祥. 结瘤信号转导的分子机制与CRISPR/Cas9技术在百脉根中的应用研究[D]. 武汉: 华中农业大学, 2019.
[8] CAMILLE F F, SOVANNA T, MAĚL B, et al. DELLA-mediated gibberellin signalling regulates Nod factor signalling and rhizobial infection[J]. Nature Communications,2016,7(1):119-144. DOI:10.1038/ncomms12636.
[9] MIYATA K, KAWAGUCHI M, NAKAGAWA T. Two distinct EIN2 genes cooperatively regulate ethylene signaling in Lotus japonicus.[J]. Plant & Cell Physiology,2013,54(9):1469-77. DOI:10.1093/pcp/pct095.
[10] GUINEL F C. Ethylene, a hormone at the center-stage of nodulation[J]. Frontiers in Plant Science, 2015, 6:1121. DOI:10.3389/fpls.2015.01121.
[11] 王俊斌, 王海凤, 樊明寿. 茉莉酸对植物根瘤形成和发育的调控作用[J]. 中国农学通报, 2010(17):191-193. DOI:CNKI:SUN:ZNTB.0.2010-17-043.
[12] 刘薇, 赵振芳, 冯永君, 等. 植物激素在豆科植物根瘤形成和发育过程中的调控作用[J]. 大豆科学, 2013, 32(2): 262-266. DOI:10.3969/j.issn.1000-9841.2013.02.028.
[13] DING Y L, KALO P, YENDREK C, et al. Abscisic acid coordinates nod factor and cytokinin signaling during the regulation of nodulation in Medicago truncatula [J]. Plant Cell, 2008, 20(10):2681-2695. DOI:10.1105/tpc.108.061739.
[14] CAI Z M, WANG Y N, ZHU L, et al. GmTIR1/GmAFB3-based auxin perception regulated by miR393 modulates soybean nodulation[J]. New Phytol, 2017, 215(2): 672-686. DOI:10.1111/nph.14632.
[15] GONZALEZ A A, AGBÉVINOU K, HERRBACH V, et al. Abscisic acid promotes pre-emergence stages of lateral root development in Medicago truncatula[J]. Plant Signal Behav, 2015, 10(1): e977741. DOI:10.4161/15592324.2014.977741.
[16] ROMERO E, PLAZA C, SENESI N, et al. Humic acid-like fractions in raw and vermicomposted winery and distillery wastes[J]. Geoderma, 2007, 139(3/4): 397-406. DOI:10.1016/j.geoderma.2007.03.009.
[17] 张祥, 梁济, 李接励, 等. 腐殖酸肥料对小麦氮素吸收利用的影响[J]. 化肥工业, 2019, 46(3): 61-66. DOI:10.3969/j.issn.1006-7779.2019.03.016.
[18] 张永霞, 张英杰, 巩冠群, 等. 黄腐酸对植物生长作用效果研究[J]. 应用化工, 2021, 50(4): 1069-1072,1076. DOI:10.16581/j.cnki.issn1671-3206.20210127.013.
[19] REZAZADEH M K, NEJAD T S, SHOKOUH F R A. The effect of different levels of humic acid fertilizer on components of biological nitrogen fixation in cowpea cultivars in Ahvaz.[J]. Int J Biosci, 2014,5(2):167-174. DOI: 10.12692/ijb/5.2.167-174.
[20] 邱小倩, 许原原, 王若楠, 等. 黄腐酸对苜蓿固氮效率及产量的影响研究[J]. 腐植酸, 2018(4): 33-36. DOI:10.19451/j.cnki.issn1671-9212.2018.04.005.
[21] 张运婷. 外源磷酸二氢钾和黄腐酸对紫花苜蓿根瘤菌侵染、运移及定殖的影响[D]. 兰州: 甘肃农业大学, 2020.
[22] 高同国, 袁红莉, 侯慧云, 等. 腐植酸类物质在氮循环中作用的研究进展[J]. 腐植酸, 2020(3): 11-18. DOI:10.19451/j.cnki.issn1671-9212.2020.03.001.
[23] HARTMANN A, ROTHBALLER M, HENSE B A, et al. Bacterial quorum sensing compounds are important modulators of microbe-plant interactions[J]. Front Plant Sci, 2014, 5: 131. DOI:10.3389/fpls.2014.00131.
[24] 高同国.低阶煤降解微生物菌群的筛选及降解产生的黄腐酸诱导大豆结瘤固氮的机理[D].北京:中国农业大学,2012.
[25] CAPSTAFF N M, MORRISON F, CHEEMA J, et al. Fulvic acid increases forage legume growth inducing preferential up-regulation of nodulation and signalling-related genes[J]. J Exp Bot, 2020, 71(18): 5689-5704. DOI:10.1093/jxb/eraa283.
[26] 陈雪梅, 王沙生. HPLC法定量分析植物组织中ABA, IAA和NAA[J]. 植物生理学通讯, 1992, 28(5): 368-371. DOI:10.13592/j.cnki.ppj.1992.05.024.
[27] 刘旭娇. 豆科植物根瘤的发育及其自我调整[J]. 安徽农业科学, 2013, 41(34): 13110-13114. DOI:10.13989/j.cnki.0517-6611.2013.34.003.
[28] 张婧. 1,3-二(2-吡啶基)脲对根瘤菌结瘤特性的影响[D]. 太原: 山西大学, 2011.
[29] 张伟. 枫香拟茎点霉B3促进花生结瘤的生理和激素信号分子机制研究[D]. 南京: 南京师范大学, 2016.
[30] WASSON A P, PELLERONE F I, MATHESIUS U. Silencing the flavonoid pathway in Medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia[J]. Plant Cell, 2006, 18(7): 1617-1629. DOI:10.1105/tpc.105.038232.
[31] NG J L P, HASSAN S, TRUONG T T, et al. Flavonoids and auxin transport inhibitors rescue symbiotic nodulation in the Medicago truncatula cytokinin perception mutant cre1[J]. Plant Cell, 2015, 27(8): 2210-2226. DOI:10.1105/tpc.15.00231. (