谷子抽穗期突变体jt1242-14b的遗传分析和定位

doi:10.3969/j.issn.1000-1565.2023.01.013

河北大学学报(自然科学版) ›› 2023, Vol. 43 ›› Issue (1): 87-94.DOI: 10.3969/j.issn.1000-1565.2023.01.013

谷子抽穗期突变体jt1242-14b的遗传分析和定位

王春芳¹,祁东梅¹,史慎奎¹,张温典¹,智慧²,刁现民²

收稿日期:2022-04-26 出版日期:2023-01-25 发布日期:2023-02-22
通讯作者: 刁现民(1963—)
作者简介:王春芳(1983—),女,河北丰宁人,河北民族师范学院副教授,博士,主要从事分子育种方面的研究.
E-mail: wangchunfang18@126.com
基金资助:
河北省高等学校科学技术研究项目(ZD2022151;QN2022188);2021年度河北民族师范学院青年基金项目(QN2021002);2021年度承德市科技局研究项目(202109A196)

Genetic analysis and mapping of a heading date mutant jt1242-14b in Setaria italica

WANG Chunfang¹, QI Dongmei¹, SHI Shenkui¹, ZHANG Wendian¹, ZHI Hui², DIAO Xianmin²

1.College of Biological and Food Science, Hebei Normal University for Nationalities, Chengde 067000, China; 2.Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received:2022-04-26 Online:2023-01-25 Published:2023-02-22

摘要/Abstract

摘要： 为鉴定控制谷子抽穗的关键基因,通过甲基磺酸乙酯(ethyl methyl sulfonate,EMS)诱变谷子参考基因组测序品种豫谷1号,获得遗传稳定的谷子超早抽穗突变体jt1242-14b.与豫谷1号相比,jt1242-14b抽穗期明显提前,茎秆变细,叶片变窄、变短.遗传分析表明,突变性状受隐性单基因控制.以SSR41(父本)、jt1242-14b(母本)和F₂群体进行突变基因定位,结果表明,该基因位于第9号染色体,在分子标记In4746和In9-11之间的4 447 kb之内.进一步测序比对分析发现突变位点位于PHYB基因内部,因此,PHYB很可能是该早熟突变体的目标基因.本研究为谷子早抽穗基因克隆及PHYB基因功能研究提供材料基础.

关键词: 谷子, 抽穗期, 遗传分析, 基因定位

Abstract: To identify the target genes controlling heading date, an early heading mutant jt1242-14b was identified from the ethyl methyl sulfonate(EMS)induced mutant library of the foxtail millet variety Yugu1. Compared with Yugu1, jt1242-14b showed an early heading date, thinner stem, and narrow and short leaves. Genetic analysis showed that the mutant was controlled by a recessive gene. Genetic mapping of the mutant gene was conducted from segregating population of jt1242-14b/SSR41. The gene was mapped in an interval of 4 447 kb between the molecular marker In4746 and In9-11 on chromosome 9. Finally, jt1242-14b was caused by a mutation in PHYB gene revealed by sequencing, so PHYB could be the target gene of the early heading mutant. This study provided a material foundation for cloning and functional research of PHYB in millet early heading genes.

Key words: Setaria italica, heading date, genetic analysis, genetic mapping

中图分类号:

S131

王春芳,祁东梅,史慎奎,张温典,智慧,刁现民. 谷子抽穗期突变体jt1242-14b的遗传分析和定位[J]. 河北大学学报(自然科学版), 2023, 43(1): 87-94.

WANG Chunfang, QI Dongmei, SHI Shenkui, ZHANG Wendian, ZHI Hui, DIAO Xianmin. Genetic analysis and mapping of a heading date mutant jt1242-14b in Setaria italica[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(1): 87-94.

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谷子抽穗期突变体jt1242-14b的遗传分析和定位

Genetic analysis and mapping of a heading date mutant jt1242-14b in Setaria italica

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