建立了用液相色谱-串联质谱(LC-MS-MS)测定蜂蜜中9种青霉素残留量的分析方法.3g蜂蜜样品用25 mL pH=8.5的0.15 mol/L磷酸二氢钠缓冲溶液溶解,用Oasis HLB固相萃取柱净化,目标物用乙腈-水(体积比1:1)洗脱,定容收集液至3 mL.采用电喷雾电离,正离子扫描,多反应监测(MRM)模式检测.0.5,1.0,2.0,5.0 μg/kg 4个添加水平的回收率为76.9％～104.3％;相对标准偏差为3.29％～9.12％;方法定量限是:青霉素G、乙氧萘青霉素为0.5 μg/kg,氧哌嗪青霉素、青霉素V、苯唑青霉素为1.0 μg/kg,羟氨苄青霉素、氨苄青霉素、邻氯青霉素、双氯青霉素为2.0 μg/kg.
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