为了探寻一种高效的灵芝多糖提取方法,采用单因子分析和正交实验,对弱碱性介质下微波法提取灵芝多糖进行了优化研究.各因素对灵芝多糖提取的影响依次为提取时间,pH值,固液质量比,预浸时间.最适条件为预浸1.5 h,提取15 min,固液质量比1∶110,pH 8.0,提取2次.灵芝多糖提取率为3.200%,结果表明:该方法能显著提高灵芝多糖的提取率.
The optimization of the extraction process the Ganoderma lucidum polyscaccharides(GLP) was studied. The microwave method under slightly alkaline medium was employed and a single factor analysis and an orthogonal test were used in the study. The order of the effects of the factors influencing the extraction was as follow: extraction time, pH, the mass ratio between G. lucidum and water, presoak time. The optimum condition was: presoak for 1.5 h, extract for 15 min, the mass ratio of G. lucidum and water 1∶110, pH 8.0 and extracted twice. GLP extraction rate of 3.200%, the result indicated that the method can significantly improve the rate of extraction of GLP.
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