7-羟基香豆素醛在检测次氯酸根中的应用

  • 阴彩霞 ,
  • 熊康明 ,
  • 霍方俊
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阴彩霞(1978—),女,山西平遥人,山西大学教授,主要从事有机小分子荧光探针的研究. E-mail:yincx@sxu.edu.cn

收稿日期: 2017-10-28

  网络出版日期: 2018-01-25

基金资助

国家自然科学基金资助项目(21672131;21775096;21705102);山西省人才支持计划资助项目(2014401);山西省归国基金资助(2017-026)

Application of 7-hydroxylcoumarin-aldehyde in the detection of hypochlorite

  • YIN Caixia ,
  • XIONG Kangming ,
  • HUO Fangjun
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  • 1.Institute of Molecular Science, Shanxi University, Taiyuan 030006, China; 2.Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China

Received date: 2017-10-28

  Online published: 2018-01-25

摘要

7-羟基香豆素醛在PBS(pH=7.4)的缓冲溶液中可以被用于检测次氯酸根.光谱实验结果表明,该探针在PBS(pH=7.4)的缓冲溶液中对次氯酸根的识别具有荧光增强的性质,并伴随着溶液的颜色由浅黄色变为深黄色,溶液的荧光由弱荧光变为深蓝色荧光.探针对次氯酸根的识别响应在1 s内迅速完成,并且检测线可以达到51.75 nmol/L.此外,7-羟基香豆素醛可以在HepG2细胞中对次氯酸根进行荧光成像.

本文引用格式

阴彩霞 , 熊康明 , 霍方俊 . 7-羟基香豆素醛在检测次氯酸根中的应用[J]. 河北大学学报(自然科学版), 2018 , 38(1) : 28 -32 . DOI: 10.3969/j.issn.1000-1565.2018.01.005

Abstract

7-hydroxylcoumarin-aldehyde can be used to detect hypochlorite in PBS(pH=7.4)solution.The results of spectra show that the probe has a fluorescence enhancement property for hypochlorite recognition in PBS(pH=7.4)solution, and the color of the solution changes from light yellow to deep-yellow, and the fluorescence of the solution changes from weak fluorescence to deep-blue fluorescence.The recognition response of the probe to hypochlorite can be rapidly completed within 1s, and the detection limit is found to be as low as 51.75 nmol/L.In addition, the probe can be used for fluorescence imaging of hypochlorite in HepG2 cells.

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