小分子生物硫醇半胱氨酸(Cys)、同型半胱氨酸(Hcy)及谷胱甘肽(GSH)在人体中扮演着重要的角色.由于荧光探针法对硫醇的检测具有选择性好、灵敏度高、响应时间快、可实现生物体内硫醇的检测等优势,本文基于亲核加成反应机理,设计合成了一种新的选择性检测硫醇的荧光探针.该探针与硫醇作用后,荧光强度随硫醇浓度的增加逐渐增强,2 min内荧光强度可达到最大值.研究结果表明,该探针在生物成像方面有潜在的应用价值.
Biothiols, cysteine(Cys)and homocysteine(Hcy)and glutathione(GSH)play key roles in human body. Owing to the advantages of fluorescence probe method, such as high sensitivity, excellent selectivity, fast response time and the ability to detect thiols in vivo, a new fluorescence probe for the selective detection of biothiols based on the nucleophilic addition mechanism was designed and synthesized in this paper. Upon addition of biothiols, the fluorescence intensity increased with the increase of thiol concentration and reached its maximum within 2 min. The results showed that the probe has potential application in bioimaging.
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