以紫色红曲霉S1为出发菌株进行微波诱变,筛选出产红曲色素能力最高的突变株紫色红曲霉S1-29,其红曲色素值达3 240 U/g,比出发菌株提高了57%.针对菌株S1-29固态发酵培养产红曲色素的条件进行优化,添加不同的碳源、氮源以及无机盐,研究添加物对产红曲色素能力的影响,并进行正交实验,得到最佳发酵条件:在大米基质中添加葡萄糖、蛋白胨、硫酸镁和磷酸氢二钾,使其质量分数分别为4%,6%,0.4%和0.3%,32 ℃培养11 d,菌株S1-29红曲色素值达到4 003 U/g,比优化前提高23.55%.
The microwave mutagenesis was carried out when Monascus purpureus S1 was used as the original strain. M.purpureus S1-29 was screened with the highest Monascus pigment value of 3 240 U/g dry base, which was increased by 57% compared with that of the original strain. The conditions for the production of Monascus pigment by solid-state fermentation of strain S1-29 were optimized. The effects of carbon sources, nitrogen sources and inorganic salts on pigment production were investigated and orthogonal experiments were carried out. The results showed that when 4% glucose, 6% peptone, 0.4% magnesium sulfate and 0.3% dipotassium hydrogen phosphate were added into the rice media and cultured at 32 ℃ for 11 days, the Monascus pigment value of M.purpureus S1-29 reached 4 003 U/g dry base, which was increased by 23.55% compared with that of control.
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