建立一种流动注射化学发光法测定阿卡波糖的新方法.在碱性条件下,阿卡波糖对H2O2-鲁米诺发光体系的发光强度具有显著的增敏作用,在最佳实验条件下,发光强度的增加值(ΔI)与阿卡波糖的质量浓度在3.0×10-4~2.0×10-2 mg/mL内成线性关系,相关系数为0.998 7.方法的检出限为2.3×10-4 mg/mL,对0.01 mg/mL的阿卡波糖连续测定11次,其相对标准偏差为2.7%,并应用于阿卡波糖片剂含量的测定,平均回收率为101.6%.本方法操作简单快速,重现性好,可用于阿卡波糖的含量测定.
A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H2O2 in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10-4~2×10-2 mg/mL with a correlation coefficient of 0.998 7. The detection limit was 2.3×10-4 mg/mL and RSD(n=11)is 2.7% for 0.01 mg/mL acarbose. The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%.
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