流动注射化学发光法测定阿卡波糖

doi:10.3969/j.issn.1000-1565.2022.03.005

河北大学学报(自然科学版) ›› 2022, Vol. 42 ›› Issue (3): 251-255.DOI: 10.3969/j.issn.1000-1565.2022.03.005

流动注射化学发光法测定阿卡波糖

赵冕¹,王贝贝¹,王冰²,李献锐²

收稿日期:2021-10-20 出版日期:2022-05-25 发布日期:2022-06-16
通讯作者: 李献锐(1969—)
作者简介:赵冕(1986—),女,河北晋州人,河北医科大学实验师,主要从事化学分析研究.E-mail:18601529@hebmu.edu.cn
基金资助:
河北省自然科学基金资助项目(B2020206009);河北省高等学校科学技术研究项目(QN2021101)

Flow injection chemiluminescence method for determination of acarbose

ZHAO Mian¹, WANG Beibei¹, WANG Bing², LI Xianrui²

1.Experimental Center for Teaching, Hebei Medical University, Shijiazhuang 050017, China; 2.College of Pharmacy, Hebei Medical University, Shijiazhuang 050017, China

Received:2021-10-20 Online:2022-05-25 Published:2022-06-16

摘要/Abstract

摘要： 建立一种流动注射化学发光法测定阿卡波糖的新方法.在碱性条件下,阿卡波糖对H₂O₂-鲁米诺发光体系的发光强度具有显著的增敏作用,在最佳实验条件下,发光强度的增加值(ΔI)与阿卡波糖的质量浓度在3.0×10^-4~2.0×10^-2 mg/mL内成线性关系,相关系数为0.998 7.方法的检出限为2.3×10^-4 mg/mL,对0.01 mg/mL的阿卡波糖连续测定11次,其相对标准偏差为2.7%,并应用于阿卡波糖片剂含量的测定,平均回收率为101.6%.本方法操作简单快速,重现性好,可用于阿卡波糖的含量测定.

关键词: 阿卡波糖, 流动注射, 化学发光, 增敏

Abstract: A simple and rapid flow-injection chemiluminescence(FI-CL)method was developed for the determination of acarbose in tablets based on the fact that acarbose can remarkably enhance the chemiluminescence of Luminol-H₂O₂ in alkaline medium. The determination conditions were optimized. Under optimum conditions, the chemiluminescence relative intensity had a good linear relationship with acarbose concentration in the range of 3×10^-4~2×10^-2 mg/mL with a correlation coefficient of 0.998 7. The detection limit was 2.3×10^-4 mg/mL and RSD(n=11)is 2.7% for 0.01 mg/mL acarbose. The proposed method has been successfully applied for the determination of acarbose in tablets sample and the average recovery was 101.6%.

Key words: acarbose, flow injection, chemiluminescence, sensitization

中图分类号:

O657.39

赵冕,王贝贝,王冰,李献锐. 流动注射化学发光法测定阿卡波糖[J]. 河北大学学报(自然科学版), 2022, 42(3): 251-255.

ZHAO Mian, WANG Beibei, WANG Bing, LI Xianrui. Flow injection chemiluminescence method for determination of acarbose[J]. Journal of Hebei University(Natural Science Edition), 2022, 42(3): 251-255.

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流动注射化学发光法测定阿卡波糖

Flow injection chemiluminescence method for determination of acarbose

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