毛细管电泳电化学发光检测香草扁桃酸和高香草酸

doi:10.3969/j.issn.1000-1565.2016.03.007

河北大学学报(自然科学版) ›› 2016, Vol. 36 ›› Issue (3): 257-263.DOI: 10.3969/j.issn.1000-1565.2016.03.007

毛细管电泳电化学发光检测香草扁桃酸和高香草酸

杨洋,孙雪梅,李传龙

收稿日期:2015-08-23 出版日期:2016-05-25 发布日期:2016-05-25
通讯作者: 孙雪梅(1967—),女,山东青岛人,青岛科技大学副教授,主要从事毛细管电泳研究.E-mail:2634702755@qq.com
作者简介:杨洋(1990—),女,河北沧州人,青岛科技大学在读硕士研究生. E-mail:1193428612@qq.com
基金资助:
山东省自然科学基金资助项目(ZR2009BM029)

Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection

YANG Yang,SUN Xuemei,LI Chuanlong

Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry andMolecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China

Received:2015-08-23 Online:2016-05-25 Published:2016-05-25

摘要/Abstract

摘要： 利用毛细管电泳电化学发光(CE-ECL)法检测了尿样中的香草扁桃酸(VMA)和高香草酸(HVA),得到了最佳的分离检测条件:50 mmol/L磷酸盐缓冲溶液(pH 8.2);分离电压16 kV;检测电势1.2 V(相对于饱和甘汞电极).在最佳条件下,VMA和HVA的浓度检测限(S/N=3)分别为5.0×10^-7 mol/L和6.1×10^-7 mol/L,线性回归系数分别为0.999 3和0.997 9.并以此方法将尿液中的VMA和HVA在10 min内有效地分离检测,不受其他干扰,得到加标回收率分别为98%和99%,取得了满意的结果.

关键词: 毛细管电泳, 电化学发光检测, 香草扁桃酸, 高香草酸

Abstract: Vanilla mandelic acid(VMA)and homovanillic acid(HVA)in the urine were detected by capillary electrophoresis with electrochemiluminescence detection(CE-ECL).The following conditions were suitable for the determination of VMA and HVA:running buffer,50 mmol/L NaH₂PO₄-Na₂HPO₄(pH=8.2);separation voltage,16 kV;detection potential,1.2 V(vs.saturated calomel electrode(SCE)).Under the optimum conditions of detection,satisfactory sensitivities(LOD of 5.0×10^-7 mol/L for VMA and 6.1×10^-7 mol/L for HVA at S/N=3)were obtained,the correlation coefficients of the method were 0.999 3 and 0.997 9,respectively.The determination of these metabolites in human urine was completed within 10 min without any interferences,the recoveries were VMA 98% and HVA 99%,the result obtained is satisfactory.

Key words: capillary electrophoresis, electrochemiluminescence detection, vanilla mandelic acid, homovanillic acid

中图分类号:

O657.8

杨洋,孙雪梅,李传龙. 毛细管电泳电化学发光检测香草扁桃酸和高香草酸[J]. 河北大学学报(自然科学版), 2016, 36(3): 257-263.

YANG Yang,SUN Xuemei,LI Chuanlong. Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(3): 257-263.

参考文献

[1] SADILKOVA K,DUGAW K,BENJAMIN D,et al.Analysis of vanillylmandelic acid and homovanillic acid by UPLC–MS/MS in serum for diagnostic testing for neuroblastoma[J].Clinica Chimica Acta,2013,424:253-257.DOI:10.1016/j.cca.2013.06.024.
[2] 许育丹.重氮法和微柱比色法检测尿液香草扁桃酸的方法比较[J].实用医技杂志,2005,12(03A):578-579.
[3] 陈曼娜,廖瑛.微柱比色法检测尿液VMA含量的评价[J].现代检验医学杂志,2003,18(1):14-15. CHEN Manna,LIAO Ying.To envalue the way of using micro-column chromatography determine VMA in the urine[J].Journal Of Modern Laboratory Medicine,2003,18(1):14-15.
[4] TOKUDA T,TOKIEDA T,ANAZAWA A,et al.Simultaneous determination of vanillylmandelic acid,homovanillic acid and creatinine in urine by reversed-phase ion-pair chromatography[J].Journal of Chromatography B:Biomedical Sciences And Applications,1990,530:418-423.DOI:10.1016/s0378-4347(00)82344-0.
[5] 张华,李永兴.高效液相电化学法检测尿儿茶酚胺和VMA诊断嗜铬细胞瘤[J].现代检验医学杂志,2003,18(3):8-9. ZHANG Hua,LI Yongxing.Urinary catecholamines and VMA measured by high performance liquid chromatography(HPLC)-electrochemical detector(ECD)for diagnosis of pheochromocytoma[J].Journal of Modern Laboratory Medicine,2003,18(3):8-9.
[6] DENG Biyang,SU Caina,KANG Yanhui.Determination of norfloxacin in human urine by capillary electrophoresis with electrochemiluminescence detection[J].Analytical And Bioanalytical Chemistry,2006,385(7):1336-1341.DOI:10.1007/s00216-006.
[7] AN Dong,CHEN Zhuqiu,ZHENG Jiachun,et al.Determination of biogenic amines in oysters by capillary electrophoresis coupled with electrochemiluminescence[J].Food Chemistry,2015,168:1-6.DOI:10.1016/j.foodchem.2014.07.019.
[8] LI Jianguo,YAN Qinyi,GAO Yunlong,et al.Electrogenerated chemiluminescence detection of amino acids based on precolumn derivatization coupled with capillary electrophoresis separation[J].Analytical Chemistry,2006,78(8):2694-2699.DOI:10.1021/ac052092m.
[9] 高萍,徐佳,孙玉倩,等.毛细管电泳检测尿儿茶酚胺代谢产物及在诊断嗜铬细胞瘤中的应用[J].哈尔滨医科大学学报,2008,42(5):511. GAO Ping,XU Jia,SUN Yuqian,et al.Determination of urine catecholamine metabolic products by capillary electrophoresis for diagnosis of pheochromocytoma[J].Journal Of Harbin Medical University,2008,42(5):511.
[10] JIN Wenrui,WENG Qianfeng,WU Jianren.Determination of bovine serum albumin by capillary zone electrophoresis with end-column amperometric detection at the carbon fiber microdisk array electrode[J].Analytica Chimica Acta,1997,342(1):67-74.DOI:10.1016/s0003-2670(96)00530-2.
[11] JIN Wenrui,YU Daiqing,DONG Qian,et al.A new method of quantification of pipemidic acid by capillary zone electrophoresis with end-column amperometric detection[J].Electrophoresis,2000,21(5):925-929.

[1]	范保瑞,马逢伯,刘卉,邱明恒,李海鹰. 半仿生法提取夏枯草中熊果酸和齐墩果酸[J]. 河北大学学报(自然科学版), 2019, 39(6): 605-610.
[2]	李美婷,陈跃,李亚茹,李怀栋, 周灿,褚晓雪,张红医. 动态pH连接-毛细管电泳法测定和表征饮品中7种酚酸[J]. 河北大学学报(自然科学版), 2019, 39(6): 594-604.
[3]	孙汉文,夏祥华. 径向基函数神经网络用于毛细管电泳同时检测水中苯二酚、苯酚和对硝基苯酚[J]. 河北大学学报(自然科学版), 2013, 33(3): 252-257.
[4]	邵钰秀,余云娟,肖晶,陶香香,陈冠华. 电堆积毛细管电泳法检测食品中四环素类抗生素残留[J]. 河北大学学报(自然科学版), 2013, 33(2): 161-166.
[5]	李志伟,赵云超,齐建敏. 高效毛细管电泳法测定缬沙坦苯磺酸氨氯地平[J]. 河北大学学报(自然科学版), 2012, 32(2): 149-153.
[6]	孙汉文,问海芳,苏明. 毛细管电泳-联吡啶钌电化学发光测定利多卡因[J]. 河北大学学报(自然科学版), 2010, 30(6): 652-656.
[7]	张红医,王艳霞,景聪,陈辉. 中药毛细管电泳谱图的可视化比照[J]. 河北大学学报(自然科学版), 2010, 30(2): 152-157.
[8]	刘娜,孙汉文,何攀. 毛细管电泳法测定复方乳酸环丙沙星注射液[J]. 河北大学学报(自然科学版), 2010, 30(1): 69-72,77.
[9]	何攀,孙汉文. 毛细管电泳-前沿分析法在药物-蛋白结合研究中的应用[J]. 河北大学学报(自然科学版), 2009, 29(2): 214-224.
[10]	阎正,王春云,苑若瑶,蔡立鹏,王继坤. 长白山人参的毛细管电泳指纹图谱[J]. 河北大学学报(自然科学版), 2009, 29(1): 55-60,64.
[11]	孙汉文,赵伟,何攀. 毛细管电泳有效分离和同时检测8种氟喹诺酮[J]. 河北大学学报(自然科学版), 2008, 28(2): 152-157.
[12]	赵燕燕,王丽娟,李志平,李月秋,韩媛媛. 猪脏器中痕量喹诺酮类药物的胶束毛细管电泳在线富集技术检测[J]. 河北大学学报(自然科学版), 2007, 27(1): 35-39,58.
[13]	胡孝忠,刘保启,王玉春,丁良. 高效毛细管电泳法测定姜黄素类化合物中姜黄素的含量[J]. 河北大学学报(自然科学版), 2004, 24(5): 507-510,555.
[14]	李保会,王德先,田宝娟,杨更亮,陈义. 痕量手性药物扑尔敏的在线富集和拆分[J]. 河北大学学报(自然科学版), 2003, 23(2): 158-161.
[15]	宋秀荣,赵敬湘,杨更亮,孙汉文,尹俊发,韩亚辉. 非水相毛细管电泳法测定雷公藤次碱[J]. 河北大学学报(自然科学版), 2001, 21(3): 330-332.

毛细管电泳电化学发光检测香草扁桃酸和高香草酸

Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价