猪脏器中痕量喹诺酮类药物的胶束毛细管电泳在线富集技术检测

doi:10.3969/j.issn.1000-1565.2007.01.012

河北大学学报(自然科学版) ›› 2007, Vol. 27 ›› Issue (1): 35-39,58.DOI: 10.3969/j.issn.1000-1565.2007.01.012

猪脏器中痕量喹诺酮类药物的胶束毛细管电泳在线富集技术检测

赵燕燕¹,王丽娟²,李志平³,李月秋⁴,韩媛媛⁴

1.河北大学,药学院,河北,保定,071002;河北大学,医学部实验中心,河北,保定,071000； 2.河北大学,药学院,河北,保定,071002； 3.河北省兽药监察所,河北,石家庄,050051； 4.河北大学,医学部实验中心,河北,保定,071000

出版日期:2007-01-25 发布日期:2007-01-25
基金资助:
河北省科技攻关项目，河北省中医药管理局科研项目，河北大学校科研和教改项目，河北大学校科研和教改项目

Analysis of the Residuals of Trace Quinolones in the Pig Viscera by MEKC Using Sweeping Technique

ZHAO Yan-yan¹,WANG Li-juan²,LI Zhi-ping³,LI Yue-qiu⁴,HAN Yuan-yuan⁴

Online:2007-01-25 Published:2007-01-25

摘要/Abstract

摘要： 利用胶束毛细管电泳在线推扫(sweeping)富集技术建立分离检测猪脏器(肝脏和肾脏)中残留的痕量环丙沙星、氧氟沙星和恩诺沙星3种喹诺酮类药物的方法.采用未涂层的熔硅弹性石英毛细管(48.5 cm×75 μm,有效柱长40 cm),以硼砂(20 mmoL/L)-十二烷基硫酸钠(80 mmoL/L)(SDS)(pH=9.6)为缓冲溶液,紫外检测波长278 nm,分离电压18 kV,进样压力1.5 kPa,样品经过三氯乙酸去蛋白处理直接在线sweeping富集,在200 s内可实现对环丙沙星、氧氟沙星和恩诺沙星的分离检测,其中环丙沙星富集倍数可达600倍.环丙沙星、氧氟沙星和恩诺沙星的检测限分别为0.007,0.016,0.020 mg/L.胶束毛细管电泳在线推扫富集技术可用于动物食品中残留的痕量药物检测.

关键词: 胶束毛细管电泳, 在线推扫技术, 猪脏器, 痕量, 喹诺酮类药物

中图分类号:

O657.8

赵燕燕,王丽娟,李志平,李月秋,韩媛媛. 猪脏器中痕量喹诺酮类药物的胶束毛细管电泳在线富集技术检测[J]. 河北大学学报(自然科学版), 2007, 27(1): 35-39,58.

ZHAO Yan-yan,WANG Li-juan,LI Zhi-ping,LI Yue-qiu,HAN Yuan-yuan. Analysis of the Residuals of Trace Quinolones in the Pig Viscera by MEKC Using Sweeping Technique[J]. Journal of Hebei University (Natural Science Edition), 2007, 27(1): 35-39,58.

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猪脏器中痕量喹诺酮类药物的胶束毛细管电泳在线富集技术检测

Analysis of the Residuals of Trace Quinolones in the Pig Viscera by MEKC Using Sweeping Technique

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