采用场流分离技术探究了马铃薯(Solanum tuberosum L.)淀粉分离表征过程中样品间以及样品与超滤膜的交联,评价了载液离子强度、样品进样量、交叉流流速和聚集时间对马铃薯淀粉回收率的影响,并对结果重复性进行了考察.结果表明:在载液(含5 mmol/L NaNO3,3 mmol/L NaN3)pH 7.00,样品进样量15 μg,交叉流流速1.0 mL/min,聚集时间2.5 min的条件下,马铃薯淀粉的回收率为(97.30±0.02)%,回转半径相对标准偏差为1.18%,分子摩尔质量的相对标准偏差为5.67%.
Potato starch was separated and characterized by field-flow fractionation. The interactions between the sample and the surface of ultrafiltration membrane in AF4 were investigated. The effects of ionic strength of carrier liquid, injection mass, cross-flow rate, and focusing time on the sample recovery were evaluated. The repeatability of the results was examined. The highest recovery of potato starch(97.30±0.02)% was obtained with a carrier liquid containing 5 mmol/L NaNO3 and 3 mmol/L NaN3, pH 7.00; injection mass, 15 μg; cross-flow rate, 1.0 mL/min; focusing time, 2.5 min. The method developed in this study showed good repeatability in terms of radius of gyration and molar mass with their relative standard deviations of 1.18% and 5.67%, respectively.
[1] 庞文渌.马铃薯主粮化战略的意义与实施 [J].粮食加工, 2019,44(2):59-61.
[2] REYNIERS S,OOMS N,GOMAND S V,et al. What makes starch from potato(Solanum tuberosum L.)tubers unique:A review [J]. Comprehensive Reviews in Food Science and Food Safety,2020,19(5):2588-2612. DOI:10.1111/1541-4337.12596.
[3] ZHANG W H,SHEN S G,SONG T G,et al. Insights into the structure and conformation of potato resistant starch(type 2)using asymmetrical flow field-flow fractionation coupled with multiple detectors [J]. Food Chemistry,2021,349:129168. DOI:10.1016/j.foodchem.2021.129168.
[4] GUO P P,LI Y Q,AN J X,et al. Study on structure-function of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors:A review [J]. Carbohydrate Polymers,2019,226:115330. DOI:10.1016/j.carbpol.2019.115330.
[5] BAYART C,JEAN E,PAILLAGOT M,et al. Comparison of SEC and AF4 analytical tools for size estimation of typhoid Vi polysaccharides [J]. Analytical Methods,2019,11(37):4851-4858. DOI:10.1039/c9ay00145j.
[6] DAI S S,ZHANG W H,DOU Y W,et al. Towards a better understanding of the relationships between the structure and antitumor activity of Gastrodia elata polysaccharides by asymmetrical flow field-flow fractionation [J]. Food Research International,2021,149:110673. DOI:10.1016/j.foodres.2021.110673.
[7] BELLO-PEREZ L A,AGAMA-ACEVEDO E,LOPEZ-SILVA M,et al. Molecular characterization of corn starches by HPSEC-MALS-RI:A comparison with AF4-MALS-RI system [J]. Food Hydrocolloids,2019,96:373-376. DOI:10.1016/j.foodhyd.2019.04.067.
[8] BURCHARD W. Branched polymers Ⅱ(advances in polymer science)[M]. Berlin:Springer-Verlag,1999:113-194.
[9] GONZÁLEZ-ESPINOSA Y,SABAGH B,MOLDENHAUER E,et al. Characterisation of chitosan molecular weight distribution by multi-detection asymmetric flow-field flow fractionation(AF4)and SEC [J]. International Journal of Biological Macromolecules,2019,136:911-919. DOI:10.1016/j.ijbiomac.2019.06.122.
[10] 张文惠,蔡春雪,王静,等.非对称场流分离技术在蛋黄浆质低密度脂蛋白粒径表征中的应用 [J]. 色谱,2017,35(8):855-859. DOI:10.3724/SP.J.1123.2017.03001.
[11] WANG J,SHEN Q,ZHANG W H,et al. Study on effect of salting process on egg yolk plasma using AF4 combined with ATR-FTIR and DSC [J]. LWT - Food Science and Technology,2018,93:362-367. DOI:10.1016/j.lwt.2018.03.058.
[12] WANG Y,YANG C,NIE Y L,et al. Reinjection flow field-flow fractionation method for nanoparticle quantitative analysis in unknown and complex samples [J]. Journal of Chromatography A,2021,1638:461897. DOI:10.1016/j.chroma.2021.461897.
[13] 张靖,郭攀攀,李惠丽,等. 基于非对称场流分离技术分离表征小米淀粉 [J]. 色谱,2020,38(2):169-176. DOI:10.3724/SP.J.1123.2019.05014.
[14] 宋天歌.基于非对称场流分离技术研究淀粉粒径与其功能特性之间的关系[D].保定:河北大学,2021.
[15] ZHANG W H,WANG J,GUO P P,et al. Study on the retrogradation behavior of starch by asymmetrical flow field-flow fractionation coupled with multiple detectors [J]. Food Chemistry,2019,277:674-681. DOI:10.1016/j.foodchem.2018.11.033.
[16] BENINCASA M A,CALDWELL K D. Flow field-flow fractionation of poly(ethylene oxide): effect of carrier ionic strength and composition [J]. Journal of Chromatography A,2001,925(1-2):159-169. DOI:10.1016/s0021-9673(01)01011-1.
[17] BENINCASA M A, GIDDINGS J C. Separation and characterization of cationic,anionic,and nonionic water-soluble polymers by flow FFF:sample recovery,overloading,and ionic strength effects [J]. Journal of Microcolumn Separations,1997,9(6):479-495. DOI:10.1002/(SICI)1520-667X(1997)9:6<479::AID-MCS5>3.0.CO;2-Z.
[18] HOYOS-LEYVA J D,BELLO- PÉREZ L A,ALVAREZ-RAMIREZ J,et al. Structural characterization of aroid starches by means of chromatographic techniques [J]. Food Hydrocolloids,2017,69:97-102. DOI:10.1016/j.foodhyd.2017.01.034.
[19] ROLLAND-SABATÉ A,COLONNA P,MENDEZ-MONTEALVO M G,et al. Branching features of amylopectins and glycogen determined by asymmetrical flow field flow Fractionation coupled with multiangle laser light scattering [J]. Biomacromolecules,2007,8(8):2520-2532. DOI:10.1021/bm070024z.
[20] MOREL M H,PINCEMAILLE J,CHAUVEAN E,et al. Insight into gluten structure in a mild chaotropic solvent by asymmetrical flow field-flow fractionation(AsFlFFF)and evidence of non-covalent assemblies between glutenin and ω-gliadin [J]. Food Hydrocolloids,2020,103:105676. DOI:10.1016/j.foodhyd.2020.105676. (
