马铃薯淀粉的场流分离表征过程回收率研究

doi:10.3969/j.issn.1000-1565.2022.04.007

河北大学学报(自然科学版) ›› 2022, Vol. 42 ›› Issue (4): 376-383.DOI: 10.3969/j.issn.1000-1565.2022.04.007

马铃薯淀粉的场流分离表征过程回收率研究

张文惠¹,宋天歌¹,孙瑜珊²,申世刚¹,窦海洋²

收稿日期:2021-12-14 出版日期:2022-07-25 发布日期:2022-09-14
通讯作者: 窦海洋(1983—)
作者简介:张文惠(1990—),女,山东诸城人,河北大学在读博士研究生,主要从事场流分离方向研究.
E-mail: zhangwenhui4000@163.com
基金资助:
河北省自然科学基金资助项目(H2020201295);大学生创新创业训练计划项目(S20221075029);大中学生科技创新能力培育专项(22E50032D)

Recovery of potato strach during separation and characterization process using field-flow fractionation

ZHANG Wenhui¹, SONG Tiange¹, SUN Yushan², SHEN Shigang¹, DOU Haiyang²

1. College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2. School of Basic Medical Sciences, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-autoimmune Disease of Hebei Province, Hebei University, Baoding 071000, China

Received:2021-12-14 Online:2022-07-25 Published:2022-09-14

摘要/Abstract

摘要： 采用场流分离技术探究了马铃薯(Solanum tuberosum L.)淀粉分离表征过程中样品间以及样品与超滤膜的交联,评价了载液离子强度、样品进样量、交叉流流速和聚集时间对马铃薯淀粉回收率的影响,并对结果重复性进行了考察.结果表明:在载液(含5 mmol/L NaNO₃,3 mmol/L NaN₃)pH 7.00,样品进样量15 μg,交叉流流速1.0 mL/min,聚集时间2.5 min的条件下,马铃薯淀粉的回收率为(97.30±0.02)%,回转半径相对标准偏差为1.18%,分子摩尔质量的相对标准偏差为5.67%.

关键词: 场流分离, 马铃薯淀粉, 回收率

Abstract: Potato starch was separated and characterized by field-flow fractionation. The interactions between the sample and the surface of ultrafiltration membrane in AF4 were investigated. The effects of ionic strength of carrier liquid, injection mass, cross-flow rate, and focusing time on the sample recovery were evaluated. The repeatability of the results was examined. The highest recovery of potato starch(97.30±0.02)% was obtained with a carrier liquid containing 5 mmol/L NaNO₃ and 3 mmol/L NaN₃, pH 7.00; injection mass, 15 μg; cross-flow rate, 1.0 mL/min; focusing time, 2.5 min. The method developed in this study showed good repeatability in terms of radius of gyration and molar mass with their relative standard deviations of 1.18% and 5.67%, respectively.

Key words: field-flow fractionation, potato starch, recovery

中图分类号:

O657

张文惠,宋天歌,孙瑜珊,申世刚,窦海洋. 马铃薯淀粉的场流分离表征过程回收率研究[J]. 河北大学学报(自然科学版), 2022, 42(4): 376-383.

ZHANG Wenhui, SONG Tiange, SUN Yushan, SHEN Shigang, DOU Haiyang. Recovery of potato strach during separation and characterization process using field-flow fractionation[J]. Journal of Hebei University(Natural Science Edition), 2022, 42(4): 376-383.

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马铃薯淀粉的场流分离表征过程回收率研究

Recovery of potato strach during separation and characterization process using field-flow fractionation

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