基于场流分离技术分离表征血清中的脂蛋白

doi:10.3969/j.issn.1000-1565.2017.02.004

河北大学学报(自然科学版) ›› 2017, Vol. 37 ›› Issue (2): 128-133.DOI: 10.3969/j.issn.1000-1565.2017.02.004

基于场流分离技术分离表征血清中的脂蛋白

王静¹,张潇月²,张竞文²,窦海洋^1,2,申世刚¹

收稿日期:2016-12-05 出版日期:2017-03-25 发布日期:2017-03-25
通讯作者: 窦海洋(1983—),男,河北香河人,河北大学讲师,博士,主要从事场流分离研究.E-mail:douhaiyang-1984@163.com申世刚(1964—),男,河北阜城人,河北大学教授,博士,主要从事反应动力学研究.E-mail:shensg@hbu.edu.cn
作者简介:王静(1992—),女,河北临漳人,河北大学在读硕士研究生,主要从事场流分离研究. E-mail:13403204355@163.com
基金资助:
河北省自然科学基金资助项目(B2016201002);河北省引进留学人员资助项目(CL201603);河北省高等学校科技研究基金资助项目(QN2016081);河北省2016医学学科研究重点课题资助项目(20160048);保定市科学研究与发展计划资助项目(15ZG049);河北省大学生创新创业训练计划资助项目(201610075071)

Separation and characterization of lipoproteins in serum using field flow fractionation

WANG Jing¹,ZHANG Xiaoyue²,ZHANG Jingwen²,DOU Haiyang^1,2,SHEN Shigang¹

1.Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2.College of Medicine, Hebei University, Baoding 071002, China

Received:2016-12-05 Online:2017-03-25 Published:2017-03-25

摘要/Abstract

摘要： 采用非对称场流分离系统与多角度激光光散射检测器、荧光检测器联用技术分离表征冠心病患者血清中脂蛋白.研究了恒定交叉流流速和指数衰减交叉流流速对非对称场流分析血清的影响.利用非对称场流分离系统分离并收集了血清主要成分片段,通过动态激光光散射仪对收集的样品片段进行了表征.研究结果表明,血清中游离的蛋白质和脂蛋白能被非对称场流分离系统分开;指数衰减交叉流流速不仅缩短了分析时间,而且提高了脂蛋白亚类片段的分离度.结果证明,非对称场流分离与多角度激光光散射检测器、荧光检测器联用技术是一种潜在的血清中脂蛋白的分离表征方法.

关键词: 非对称场流分离, 血清, 脂蛋白, 冠心病

Abstract: The lipoproteins in serum were separated and characterized using asymmetrical flow field-flow fractionation(AF4)coupled with multiangle light scattering(MALS),and fluorescence(FL)detectors.The effect of programmed cross-flow rates in linear decay and exponential decay on the AF4 analysis of serum was studied.The collected AF4 fractions of the serum were characterized by dynamic light scattering(DLS).It was found that the free proteins and lipoproteins in the serum were separated by AF4.The application of exponentially decaying cross-flow rate not only reduced the time of AF4 analysis of serum,but also improved the resolution for the sub-class of lipoproteins.The results demonstrate that AF4-MALS-FL is a useful tool for the analysis of lipoproteins in serum.

Key words: asymmetrical flow field-flow fractionation, serum, lipoprotein, coronary artery disease

中图分类号:

O657

王静,张潇月,张竞文,窦海洋,申世刚. 基于场流分离技术分离表征血清中的脂蛋白[J]. 河北大学学报(自然科学版), 2017, 37(2): 128-133.

WANG Jing,ZHANG Xiaoyue,ZHANG Jingwen,DOU Haiyang,SHEN Shigang. Separation and characterization of lipoproteins in serum using field flow fractionation[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 128-133.

参考文献

[1] KRISHNAN S,HUANG J,LEE H,et al.Combined high-density lipoprotein proteomic and glycomic profiles in patients at risk for coronary artery disease[J].Journal of Proteome Research,2015,14(12):5109-5118.DOI:10.1021/acs.jproteome.5b00730.
[2] NAKAMURA T,OBATA J-E,HIRANO M,et al.Predictive value of remnant lipoprotein for cardiovascular events in patients with coronary artery disease after achievement of LDL-cholesterol goals[J].Atherosclerosis,2011,218(1):163-167.DOI:10.1016/j.atherosclerosis.2011.04.040.
[3] KINGWELL B A,CHAPMAN M J,KONTUSH A,et al.HDL-targeted therapies:progress,failures and future[J].Nature Reviews Drug Discovery,2014,13(6):445-464.DOI:10.1038/nrd4279.
[4] CHECHI K,BLANCHARD P-G,MATHIEU P,et al.Brown fat like gene expression in the epicardial fat depot correlates with circulating HDL-cholesterol and triglycerides in patients with coronary artery disease[J].International Journal of Cardiology,2013,167(5):2264-2270.DOI:10.1016/j.ijcard.2012.06.008.
[5] PREZ-MNDEZ Ó,PACHECO H G,MARTÍNEZ-SÁNCHEZ C,et al.HDL-cholesterol in coronary artery disease risk:Function or structure[J].Clinica Chimica Acta,2014,429(0):111-122.DOI:10.1016/j.cca.2013.12.001.
[6] TOFT-PETERSEN A,TILSTED H,AAROE J,et al.Small dense LDL particles-a predictor of coronary artery disease evaluated by invasive and CT-based techniques:a case-control study[J].Lipids in Health and Disease,2011,10(1):21-27.DOI:10.1186/1476-511X-10-21.
[7] GIDDINGS J C.Field-flow fractionation:Analysis of macromolecular,colloidal,and particulate materials[J].Science,1993,260(5113):1456-1465.DOI:10.1126/science.8502990.
[8] LEE J Y,BYEON S K,MOON M H.Profiling of oxidized phospholipids in lipoproteins from patients with coronary artery disease by hollow fiber flow field-flow fractionation and nanoflow liquid chromatography–tandem mass spectrometry[J].Analytical Chemistry,2015,87(2):1266-1273.DOI:10.1021/ac503973p.
[9] NILSSON L,LEEMAN M,WAHLUND K-G,et al.Mechanical degradation and changes in conformation of hydrophobically modified starch[J].Biomacromolecules,2006,7(9):2671-2679.DOI:10.1021/bm060367h.
[10] BAALOUSHA M,STOLPE B,LEAD J R.Flow field-flow fractionation for the analysis and characterization of natural colloids and manufactured nanoparticles in environmental systems:A critical review[J].Journal of Chromatography A,2011,1218(27):4078-4103.DOI:10.1016/j.chroma.2011.04.063.
[11] DOU H,KIM K-H,LEE B-C,et al.Preparation and characterization of cyclo-1,3,5-trimethylene-2,4,6-trinitramine(RDX)powder:Comparison of microscopy,dynamic light scattering and field-flow fractionation for size characterization[J].Powder Technology,2013,235(0):814-822.DOI:10.1016/j.powtec.2012.11.042.
[12] DOU H,LEE Y-J,JUNG E C,et al.Study on steric transition in asymmetrical flow field-flow fractionation and application to characterization of high-energy material[J].Journal of Chromatography A,2013,1304(0):211-219.DOI:10.1016/j.chroma.2013.06.051.

[1]	张菊花,陈雪,王静,侯瑞兰,申世刚,窦海洋. 基于非对称场流分离研究玉米淀粉糊化[J]. 河北大学学报(自然科学版), 2019, 39(4): 379-385.
[2]	李昊一,李冬敏,马刚,商艳丽,孙英. 牛血清白蛋白聚集体的分解动力学[J]. 河北大学学报(自然科学版), 2016, 36(1): 35-42.
[3]	武金霞，武建，隋思强，吴红月，张贺迎. 蚯蚓冻干粉对高血脂小鼠胆固醇逆转运相关基因的调节作用[J]. 河北大学学报(自然科学版), 2015, 35(2): 159-164.
[4]	刘保生,闫潇娜,杨超,张彦青,吕运开. 诺氟沙星与氧氟沙星同时与牛血清白蛋白分子作用的荧光光谱[J]. 河北大学学报(自然科学版), 2011, 31(3): 262-266.
[5]	柳峰松,王晓菲,李婷,郑立,谢松. 中国明对虾CrusFc-2基因的重组表达及抗血清制备[J]. 河北大学学报(自然科学版), 2011, 31(2): 181-185.
[6]	王改珍,苗凤智,刘英华. Pb2+与人血清白蛋白的相互作用[J]. 河北大学学报(自然科学版), 2010, 30(4): 394-399.
[7]	柳峰松,李婷,王晓菲,唐婷. 中国明对虾ALFFc基因的原核表达与抗血清制备[J]. 河北大学学报(自然科学版), 2010, 30(1): 88-92.
[8]	石红梅,徐向东,时婧,赵心,朱鹤,康维钧. 固相萃取-高效液相色谱法测定人体血清中游离皮质醇[J]. 河北大学学报(自然科学版), 2009, 29(4): 398-402.
[9]	武金霞,甄兴航,刘立军,习洋,殷鹏. 蚯蚓冻干粉对高血脂症小鼠的降血脂作用[J]. 河北大学学报(自然科学版), 2008, 28(6): 652-655.
[10]	康旭珍,李建晴. 达旦黄与牛血清白蛋白作用的光谱特性[J]. 河北大学学报(自然科学版), 2008, 28(6): 629-634.
[11]	刘保生,刘智超,高静. 红霉素与牛血清白蛋白的相互作用机制[J]. 河北大学学报(自然科学版), 2005, 25(4): 380-382.
[12]	孙艳梅,刘保生,张红医,王甫丽,赵长容. 苯酚磺酞类酸性染料与牛血清白蛋白的结合反应[J]. 河北大学学报(自然科学版), 2004, 24(6): 619-623.
[13]	刘保生,高静,张红医,王甫丽,赵长容,乔娟. 溴甲酚紫与血清白蛋白的相互结合反应[J]. 河北大学学报(自然科学版), 2004, 24(4): 373-376.
[14]	赵长容,刘保生,张红医. 酸性棕染料与蛋白结合反应的研究(Ⅰ)[J]. 河北大学学报(自然科学版), 2004, 24(1): 58-60,64.
[15]	刘保生,张红医,甄志斌,王甫丽. 氯酚红与人血清白蛋白的结合[J]. 河北大学学报(自然科学版), 2003, 23(1): 38-40.

基于场流分离技术分离表征血清中的脂蛋白

Separation and characterization of lipoproteins in serum using field flow fractionation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价