基于非对称场流分离研究玉米淀粉糊化

doi:10.3969/j.issn.1000-1565.2019.04.008

河北大学学报(自然科学版) ›› 2019, Vol. 39 ›› Issue (4): 379-385.DOI: 10.3969/j.issn.1000-1565.2019.04.008

基于非对称场流分离研究玉米淀粉糊化

张菊花¹,陈雪²,王静¹,侯瑞兰²,申世刚¹,窦海洋^1,2

收稿日期:2018-12-18 出版日期:2019-07-25 发布日期:2019-07-25
通讯作者: 窦海洋(1983—),男,河北香河人,河北大学副教授,博士,主要从事场流分离研究.E-mail:douhaiyang-1984@163.com申世刚(1964—),男,河北阜城人,河北大学教授,博士,主要从事食品安全评价及分析.E-mail: shensg@hbu.edu.cn
作者简介:张菊花(1993—),女,河北涉县人,河北大学在读硕士研究生,主要从事场流分离研究. E-mail:zhang18330027828@163.com
基金资助:
河北省自然科学基金资助项目(B2016201002);河北省引进留学人员资助项目(CL201603);河北省大学生创新创业训练计划项目(2018171);河北省高等学校科学技术研究重点资助项目(ZD2019009)

Study on maize starch pasting by asymmetrical flow field-flow fractionation

ZHANG Juhua¹, CHEN Xue², WANG Jing¹, HOU Ruilan², SHEN Shigang¹, DOU Haiyang^1,2

1.Key Laboratory of Analytical Science and Technology of Hebei Province, College ofChemistry and Environmental Science, Hebei University, Baoding 071002, China; 2.College of Medicine, Hebei University, Baoding 071002, China

Received:2018-12-18 Online:2019-07-25 Published:2019-07-25

摘要/Abstract

摘要： 研究了氢氧化钠溶解法和二甲基亚砜溶解法对玉米淀粉结构的影响.采用非对称场流分离系统与多角度激光光散射检测器、示差折光检测器联用技术分离表征了玉米淀粉.同时,通过扫描电子显微镜、X线衍射、傅里叶变换红外光谱和差示扫描量热法对不同溶解方法得到的玉米淀粉凝胶的形貌和结构进行了表征.研究结果表明,氢氧化钠溶解法可以分解直链淀粉-脂质复合物,使非对称场流分离联用技术测得的玉米淀粉的粒径和摩尔质量分布更精确.

关键词: 玉米淀粉, 溶解, 凝胶, 非对称场流分离

Abstract: The effect of sodium hydroxide(NaOH)dissolution method and dimethyl sulfoxide(DMSO)dissolution method on the conformation of maize starch was investigated.The dissolved maize starch was separated and characterized by asymmetrical flow field-flow fractionation(AF4)coupled with multiangle light scattering(MALS)and differential refractive index(dRI)detectors.Meanwhile, the morphology and conformation of the maize starch gel was characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD), attenuated total reflection Flourier transform infrared spectroscopy(ATR-FTIR)and differential scanning calorimetry(DSC).The results revealed that the amylose-lipid complex can be disassociated by NaOH dissolution method, which makes the data(i.e.size and molar mass distributions)from AF4-MALS-dRI more accurate.

Key words: maize starch, dissolution, gel, asymmetrical flow field-flow fractionation

中图分类号:

O657

张菊花,陈雪,王静,侯瑞兰,申世刚,窦海洋. 基于非对称场流分离研究玉米淀粉糊化[J]. 河北大学学报(自然科学版), 2019, 39(4): 379-385.

ZHANG Juhua, CHEN Xue, WANG Jing, HOU Ruilan, SHEN Shigang, DOU Haiyang. Study on maize starch pasting by asymmetrical flow field-flow fractionation[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(4): 379-385.

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基于非对称场流分离研究玉米淀粉糊化

Study on maize starch pasting by asymmetrical flow field-flow fractionation

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