在血红素的催化作用下,青蒿素可以氧化鲁米诺使其发光.赤藓糖醇对体系的发光强度具有显著的抑制作用,且发光信号的降低值ΔI与赤藓糖醇的质量浓度在一定范围内呈线性关系,基于此建立了一种化学发光测定赤藓糖醇的新方法.在最佳实验条件下,方法的线性为0.028~2.0 mg/mL(R2=0.997 8),方法的检出限为1.6×10-2 mg/mL,对0.50 mg/mL的赤藓糖醇连续测定11次,其相对标准偏差为3.2%.并应用于实际样品中赤藓糖醇含量的测定,效果较好.
Under the catalytic action of hemin, artemisinin can oxidize luminol to produce luminescence. Erythritol has a significant inhibitory effect on the luminous intensity of the system, and the decreased value of light signal has a linear relationship with the erythritol’s concentration. Based on this principle, a new method to measure erythritol was developed. Under optimum test condition, the linear range of this method is 0.028~2.0 mg/mL(R2 =0.997 8), and the detection limit of this method is 1.6×10-2 mg/mL. The standard deviation is 3.2% after measuring 0.50 mg/mL erythritol eleven times continuously.
[1] ZHANG W L, ZHANG T, JIANG B, et al. Enzymatic approaches to rare sugar production[J]. Biotechnol Adv, 2017, 35(2): 267-274.DOI:10.1016/j.biotechadv.2017.01.004.
[2] CHEN J J, HUANG W L, ZHANG T, et al.Anti-obesity potential of rare sugar d-psicose by regulating lipid metabolism in rats[J]. Food Funct, 2019, 10(5): 2417-2425. DOI:10.1039/c8fo01089g.
[3] ZHANG W L, YU SH H, ZHANG T, et al. Recent advances in d-allulose: Physiological functionalities, applications, and biological production[J]. Trend Food Sci Technol, 2016, 54: 127-137.DOI:10.1016/j.tifs.2016.06.004.
[4] NAGATA Y, MIZUTA N, KANASAKI A, et al. Rare sugars, d-allulose, d-tagatose and d-sorbose, differently modulate lipid metabolism in rats[J]. J Sci Food Agric, 2018, 98(5): 2020-2026. DOI:10.1002/jsfa.8687.
[5] 唐海尧,陈雅萍,梁茵茵,等.赤藓糖醇在岭丰糯荔枝果肉酸奶加工过程中的应用与研究[J].食品安全导刊, 2022(19): 151-154. DOI:10.16043/j.cnki.cfs.2022.19.009.
[6] 张妍,张丽,李宝磊,等.赤藓糖醇的国内外研究进展[J].饮料工业, 2022,25(5): 76-79.
[7] 李春芳.赤藓糖醇发酵条件优化的研究进展[J].食品安全导刊, 2021(19): 163-164. DOI:10.16043/j.cnki.cfs.2021.19.095.
[8] 袁野,应向贤,范光先,等.高碘酸氧化法直接测定发酵液中赤藓糖醇[J].无锡轻工大学学报, 2000,19(1): 72-75.
[9] 钱琛,杨瑞洪,黄菲.基于化学修饰金电极的赤藓糖醇快速检测[J].理化检验(化学分册),2012,48(12):1487-1489.
[10] 周帅,唐庆九,李炜玮,等.高效液相法测定赤藓糖醇的含量[J].食用菌学报, 2012,19(3): 83-85. DOI:10.16488/j.cnki.1005-9873.2012.03.001.
[11] 吴华,闵媛婷,刘立明,等.化学发光分析与高效液相色谱联用测定芦丁片剂中芦丁含量[J].理化检验:化学分册,2021,57(8):709-713.
[12] 赵冕,王贝贝,王冰,等.流动注射化学发光法测定阿卡波糖[J].河北大学学报(自然科学版), 2022,42(3): 251-255. DOI:10.3969/j.issn.1000-1565.2022.03.005.
[13] LIU F F, HE J, ZENG M L, et al. Cu-hemin metal-organic frameworks with peroxidase-like activity as peroxidase mimics for colorimetric sensing of glucose[J]. J Nanopart Res, 2016, 18(5): 106. DOI:10.1007/s11051-016-3416-z.
[14] WANG B B, KANG K, JI X P, et al.Multifunctional encapsulating gold nanoparticles into Cu-hemin/metal-organic frameworks for catechol electrochemical detection on graphene-based electrode[J]. Nano, 2020, 15(12): 2050155. DOI:10.1142/s1793292020501556. (
