Elizabethkingia miricola is the pathogen responsible for frog head tilt disease, posing a serious threat to frog aquaculture. Carvacrol has potential applications as a feed additive in aquaculture due to its broad-spectrum antibacterial properties. To evaluate the efficacy of carvacrol in the prevention and treatment of E. miricola infections, we assessed its antibacterial activity against E. miricola and further explored its mechanisms of action. The results revealed that the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of carvacrol against E. miricola were 100 μg/mL and 200 μg/mL, respectively. After treatment with carvacrol, the concentration of soluble proteins decreased significantly, while the DNA extravasation increased. Carvacrol treatment resulted in cell deformation, shrinkage, and membrane rupture, as well as a reduction in biofilm formation with a significant down-regulation- 引用格式:冯忠居,于明威,张聪,等.冲刷场地地震波形对大直径变截面单桩时程响应的影响［J］.河北大学学报(自然科学版),2026,46(4):337-348.引用格式:李义,吴司璇,陈丽菲,等.香芹酚对米尔伊丽莎白菌的抑菌活性及机制研究［J］.河北大学学报(自然科学版),2026,46(4):394-402.DOI:10.3969/j.issn.1000-1565.2026.04.007香芹酚对米尔伊丽莎白菌的抑菌活性及机制研究李义,吴司璇,陈丽菲,鲁重阳,李伟(长江大学 生命科学学院,湖北 荆州 434025)摘 要:米尔伊丽莎白菌(Elizabethkingia miricola)是蛙类“歪头病”的致病病原,对蛙类的健康养殖构成严重威胁.香芹酚具有广谱抗菌的特性,在水产养殖中有作为饲料添加剂的潜在可能.为评估香芹酚应用于防治米尔伊丽莎白菌感染的潜力,测定了其对米尔伊丽莎白菌的抑菌能力,并探究了其作用机制.结果表明:香芹酚对米尔伊丽莎白菌的最小抑菌浓度(minimun inhibitory concentration,MIC)和最小杀菌浓度(minimun bactericidal concentration,MBC)分别为100 μg/mL和200 μg/mL.经香芹酚处理后,细菌菌体可溶性蛋白浓度显著降低,DNA外渗量增加;香芹酚处理导致菌体变形、细胞膜皱缩及破裂,生物膜形成大幅降低,并且生物膜合成相关基因的表达被显著下调.上述结果表明:香芹酚对米尔伊丽莎白菌具有较好的抑菌活性,其通过影响细菌蛋白质的正常合成,破坏细胞膜的完整性以及减少生物膜的形成来抑制细菌的生长.这为香芹酚作为抗菌剂应用于防控蛙类米尔伊丽莎白菌感染提供了参考.关键词:米尔伊丽莎白菌;香芹酚;作用机制;抑菌作用;抗菌剂中图分类号:Q939.9 文献标志码:A 文章编号:1000-1565(2026)04-0394-09DOI:10.3969/j.issn.1000-1565.2026.04.007Bacteriostatic activity and mechanism of carvacrol against Elizabethkingia miricolaLI Yi, WU Sixuan, CHEN Lifei, LU Chongyang, LI Wei(College of Life Science, Yangtze University, Jingzhou 434025,China)Abstract: Elizabethkingia miricola is the pathogen responsible for frog head tilt disease, posing a serious threat to frog aquaculture. Carvacrol has potential applications as a feed additive in aquaculture due to its broad-spectrum antibacterial properties. To evaluate the efficacy of carvacrol in the prevention and treatment of E. miricola infections, we assessed its antibacterial activity against E. miricola and further explored its mechanisms of action. The results revealed that the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of carvacrol against E. miricola were 100 μg/mL and 200 μg/mL, respectively. After treatment with carvacrol, the concentration of soluble proteins decreased significantly, while the DNA extravasation increased. Carvacrol treatment resulted in cell deformation, shrinkage, and membrane rupture, as well as a reduction in biofilm formation with a significant down-regulation- 收稿日期:2025-09-16;修回日期:2025-10-21 基金项目:广西作物病虫害生物学重点实验室开放课题(22-035-31-23KF01);国家级大学生创新创业训练项目(202410489016) 第一作者:李义(2000—),男,长江大学在读硕士研究生,主要从事生物防治方向研究.E-mail:2014332889@qq.com 通信作者:李伟(1976—),男,长江大学副教授,博士,主要从事病原微生物学方向研究.E-mail:wetli@yangtzeu.edu.cn 第4期李义等:香芹酚对米尔伊丽莎白菌的抑菌活性及机制研究河北大学学报(自然科学版) 第46卷of genes related to biofilm synthesis. These findings suggest that carvacrol exhibits strong inhibitory activity against E. miricola by disrupting bacterial protein synthesis, compromising cell membrane integrity, and reducing biofilm formation. This study provides valuable data supporting the use of carvacrol as an antibacterial agent for preventing and controlling frog diseases caused by E. miricola infection.
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