Detection of microRNA by catalytic hairpin DNA assembly based on gold nanoparticles

doi:10.3969/j.issn.1000-1565.2018.02.006

Abstract

Abstract: By combining gold nanoparticles(AuNPs)with catalytic hairpin DNA assembly,we develop a new method for the detection of microRNA(miRNA).Firstly,fluorescein-labeled hairpin DNA probe P1 was modified on the surface of AuNPs.The fluorescence signal of P1 is quenched by the AuNPs.When the target miRNA is present,it can hybrid with P1 and form the P1-miRNA intermediates.At the same time,the hairpin structure of P1 is opened.Then the opened P1 can hybrid with the hairpin P2 to form a stable structure,P1-P2,while the fluorophore in P1 is far away from the AuNPs,resulting in the fluorescence emission of fluorescein.Meanwhile,the target miRNA is released and can subsequently induce the multiple assembled reactions of P1 and P2 on the surface of AuNPs.By repeating the cycle,the amplified detection of miRNA is achieved.The method shows a good linear relationship with the miRNA in the concentration range of 50 pmol/L to 2 nmol/L.The detection limit was estimated to be 39 pmol/L. The method is simple,low cost,and used for the detection of miRNA in HepG 2 cells and HeLa cells with satisfactory results.

Key words: microRNA, catalytic hairpin assembly, Au nanoparticle

CLC Number:

O657.34

CHENG Yongqiang,ZHAO Yaqing,GAO Shuxin,NIE Xueyu,ZHANG Xuange. Detection of microRNA by catalytic hairpin DNA assembly based on gold nanoparticles[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(2): 147-153.

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