Abstract: The study established the tissue culture system with the stem segment of Caragana intermedia as the explant, and the results were as follows: with the callus induction medium of MS + GA₃ 0.15 mg/L + 6-BA 0.2 mg/L + NAA 0.4 mg/L + AC 0.1 g/L + sucrose 25 g/L + agar 6 g/L, callus induction rate was 33.33%;With the induction medium of MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 25 g/L + agar 6 g/L, sheath bud induction rate was 13.33%; With rooting medium of MS + IAA 0.5 mg/L + GA₃ 0.15 mg/L + sucrose 20 g/L + agar 6 g/L, rooting rate of 60%; After rooting, the rooting seedlings were moved to a substrate with vermiculite and nutrient soil with volume ratio of 2∶1 and the survival rate was 100%.

Key words: Caragana intermedia, tissue culture, stem segment, explant