Genetic analysis and mapping of a heading date mutant jt1242-14b in Setaria italica

doi:10.3969/j.issn.1000-1565.2023.01.013

Abstract

Abstract: To identify the target genes controlling heading date, an early heading mutant jt1242-14b was identified from the ethyl methyl sulfonate(EMS)induced mutant library of the foxtail millet variety Yugu1. Compared with Yugu1, jt1242-14b showed an early heading date, thinner stem, and narrow and short leaves. Genetic analysis showed that the mutant was controlled by a recessive gene. Genetic mapping of the mutant gene was conducted from segregating population of jt1242-14b/SSR41. The gene was mapped in an interval of 4 447 kb between the molecular marker In4746 and In9-11 on chromosome 9. Finally, jt1242-14b was caused by a mutation in PHYB gene revealed by sequencing, so PHYB could be the target gene of the early heading mutant. This study provided a material foundation for cloning and functional research of PHYB in millet early heading genes.

Key words: Setaria italica, heading date, genetic analysis, genetic mapping

CLC Number:

S131

WANG Chunfang, QI Dongmei, SHI Shenkui, ZHANG Wendian, ZHI Hui, DIAO Xianmin. Genetic analysis and mapping of a heading date mutant jt1242-14b in Setaria italica[J]. Journal of Hebei University(Natural Science Edition), 2023, 43(1): 87-94.

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