Screening and identification of xylanase-producing endophytic bacteria of Cymbidium faberi roots

doi:10.3969/j.issn.1000-1565.2017.05.011

Abstract

Abstract: In order to obtain the xylanase-producing functional strains, 592 endophytic bacteria from Cymbidium faberi roots were screened by using the method of Kongo red dye according to the size of hydrolyzed circle. The xylanase activity of strains screened was re-screened by DNS. The xylanase-producing strain was identified based on its morphological, physiological and biochemical characteristics, mole fraction of(G+C)and 16S rRNA gene sequence analysis. The results showed that 31 xylanase-producing strains were obtained, which account for 5.23% of the total strains. Seven strains with high xylanase yields were screened through DNS method. Strain 6hRe76 with the highest enzyme activity was obtained, of which the activity of xylanase was 57.15 U/ mL. The strain 6hRe76 was identified as Paenibacillus kribbensis. The research provided the new source for xylanase production.

Key words: xylanase, Paenibacillus, endophytic bacteria

CLC Number:

Q939.13

MA Chunling, LI Sihan, ZHAO Dongfang, LI Guangshuo, MA Ruiyi, SUN Lei. Screening and identification of xylanase-producing endophytic bacteria of Cymbidium faberi roots[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(5): 518-522.

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