Optimization design of anaerobic fermentation with mixed materials of bean stalk and rice straw

doi:10.3969/j.issn.1000-1565.2016.01.010

Abstract

Abstract: This experiment use fresh bean stalk and natural withering rice straw as fermentation materials.On the basis of earlier single factor test,three factors five levels quadratic regression orthogonal rotating combination design was adopted in this study,and only the accumulative gas production was involved in the response value.Effects of three factors on anaerobic fermentation were analyzed,including the environment of anaerobic fermentation temperature,dry-fresh TS ratio,inoculation percentage and the mathematical model for anaerobic mixed fermentation of bean stalk and rice straw was developed.The regression equation was optimized and analyzed when the optimum conditions and interactive effects were exposed.The results showed that the quadratic model had the optimal fitting data.The sequence of factors effecting anaerobic fermentation was inoculation percentage,the environment of anaerobic fermentation temperature - 收稿日期:2015-06-25 基金项目:国家自然科学基金青年科学基金资助项目(21406083);江苏省生物质能与酶技术重点实验室资助项目(JSBEET1211) 第一作者:邓媛方(1985—),女,河南南阳人,淮阴师范学院讲师,主要从事生物质能研究.E-mail:dengyf@hytc.edu.cn 通信作者:许家兴(1986—),男,山东日照人,淮阴师范学院副教授,博士,主要从事生物质能研究.E-mail:xujiaxing@hytc.edu.cnand dry-fresh TS ratio.The optimum conditions for dry-fresh mixed-fermentation were determined as follows:the temperature was 28.97 ℃,dry-fresh TS ratio of fresh bean stalk and rice straw was 2.23∶1,inoculation percentage was 30.11% and prediction value of TS production rate was 279.789 L/kg.Through testing with related experimental data,the relative deviation of biogas production between experiment measurement and model prediction was 0.23 %(F<0.01),which proved the multiple regression model had high fitting degree and applicability.This study could provide certain theory reference for materials anaerobic digestion of mixed straw material.

Key words: biogas, straw, anaerobic digestion, model optimization

CLC Number:

X712

DENG Yuanfang,XU Jiaxing,LIU Xiaoyan,DAI Benlin,XU Jiming. Optimization design of anaerobic fermentation with mixed materials of bean stalk and rice straw[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(1): 58-64.

References

[1] 谢光辉,王晓玉,任兰天.中国作物秸秆资源评估研究现状[J].生物工程学报,2010,26(7):855-863.DOI:10.13345/j.cjb.2010.07.023. XIE Guanghui,WANG Xiaoyu,REN Lantian.China’s crop residues resources evaluation[J].Chinese Journal of Biotechnology,2010,26(7):855-863.DOI:10.13345/j.cjb.2010.07.023.
[2] 李平,龙韩威,高立洪,等.不同预处理方式下水稻秸秆厌氧消化性能比较[J].农业工程学报,2015,31(12):200-205.DOI:10.11975/j.issn.1002-6819.2015.12.027. LI Ping,LONG Hanwei,GAO Lihong,et al.Comparison of anaerobic digestion capability of rice straw with different pretreatment methods[J].Transactions of the CSAE,2015,31(12):200-205.DOI:10.11975/j.issn.1002-6819.2015.12.027.
[3] 石勇,邱凌,邵艳秋,等.小麦秸秆与红薯藤叶混合厌氧发酵特性[J].西北农业学报,2010,19(7):186-189.DOI:10.3969/j.issn.1004-1389.2010.07.040. SHI Yong,QIU Ling,SHAO Yanqiu,et al.Study on characteristics of anaerobic fermentation with wheat straw and sweet potato vine[J].Acta Agriculturae Boreali-Occidentalis Sinica,2010,19(7):186-189.DOI:10.3969/j.issn.1004-1389.2010.07.040.
[4] 刘荣厚,王远远,孙辰,等.蔬菜废弃物厌氧发酵制取沼气的试验研究[J].农业工程学报,2008,24(4):209-213.DOI:10.3321/j.issn:1002-6819.2008.04.041. LIU Ronghou,WANG Yuanyuan,SUN Chen,et al.Experimental study on biogas production from vegetable waste by anaerobic fermentation[J].Transactions of the CSAE,2008,24(4):209-213.DOI:10.3321/j.issn:1002-6819.2008.04.041.
[5] ZHANG R H,ZHANG Z Q.Anaerobic digestion of vegetable waste with an anaerobic phased solids digester system[J].Transactions of the CSAE,2002,18(5):134-139.DOI:10.3321/j.issn:1002-6819.2002.05.027.
[6] 卢恩双,宋世德,郭满才.回归通用旋转设计的几个问题[J].西北农林科技大学学报(自然科学版),2000,30(5):110-113.DOI:10.13207/j.cnki.jnwafu.2002.05.026. LU Enshuang,SONG Shide,GUO Mancai.Problems in second-order regression general rotation analysis[J].Journal of Northwest Sci-Tech University of Agriculture and Forestry,2000,30(5):110-113.DOI:10.13207/j.cnki.jnwafu.2002.05.026.
[7] 邓媛方,邱凌,黄辉,等.酶预处理对秸秆类原料厌氧发酵特性的影响[J].农业机械学报,2015,46(6):201-206.DOI:10.6041 /j.issn.1000-1298.2015.06.028. DENG Yuanfang,QIU Ling,HUANG Hui,et al.Effects of enzymatic pretreatment straw wastes on its characteristics of anaerobic digestion for biogas production[J].Transactions of the Chinese Society for Agricultural Machinery,2015,46(6):201-206.DOI:10.6041 /j.issn.1000-1298.2015.06.028.
[8] KETTUNEN R H,RINTALA J A.The effect of low temperature(5~29 ℃)and adaptation on the methanogenic activity of biomass[J].Applied Microbiology and Biotechnology,1997,48(4):570-576.DOI:10.1007/s002530051098.
[9] CHEN H,WU H Y.Optimization of volatile fatty acid production with co-substrate of food wastes and dewatered excess sludge using response surface methodology[J].Bioresource Technology,2010,101(14):5487-5493.DOI:10.1016/j.biortech.2010.02.013.
[10] NALLATHAMBI G.Effect of inoculum/substrate ration and pretreatments on methane yield from parthenium[J].Biomass and bioenergy,1995,8(1):39-44.DOI:10.1016/0961-9534(94)00086-9.
[11] JEEWON L.Biological conversion of lignocellulosic biomass to ethanol[J].Journal of Biotechnology,1997(6):323-325.DOI:10.1016/S0168-1656(97)00073-4.
[12] 邓媛方,邱凌.香菇废弃菌棒厌氧发酵产气规律正交试验[J].农业机械学报,2014,45(3):174-178.DOI:10.6041/j.issn.1000-1298.2014.03.029. DENG Yuanfang,QIU Ling.Orthogonal test and regression analysis for biogas production of shii-take cultivation waste[J] Transactions of the Chinese Society for Agricultural Machinery,2014,45(3):174-178.DOI:10.6041/j.issn.1000-1298.2014.03.029.
[13] MOLPECERES J,GUZMAN M,ABERTURAS M R,et al.Application of central composite designs to the preparation of polycaprolactone nanoparticles by solvent displacement.[J].Journal of pharmaceutical sciences,1996,85(2):206.DOI:10.1021/js950164r.
[14] AHRING B K,IBRAHIM A A,MLADENOVSKA Z.Effect of temperature increase from 55 ℃ to 65 ℃ on performance and microbial population dynamics of an anaerobic reactor treating cattle manure[J].Water Research,2001,35:2446-2452.DOI:10.1016/S0043-1354(00)00526-1.
[15] YOUNG Chaesong.Mesophilic and thermophilic temperature co-phase anaerobic digestion compared with single-stage mesophilic and thermophilic digestion of sewage sludge[J].Water Research,2004,38:1653-1662.DOI:10.1016/j.watres.2003.12.019.
[16] LOPES W S,LEITE V D, PRASAD S.Influence of inoculum on performance of anaerobic reactors for treating municipal solid waste[J].Bioresource Technology,2004,94(3):261-266.DOI:10.1016/j.biortech.2004.01.006.
[17] HASHIMOTO A G.Effect of inoculum/substrate ratio on methane yield and production rate[J].Biological Wastes,1989,28(89):247-255.DOI:10.1016/0269-7483(89)90108-0.
[18] GUNASEELAN V N.Anaerobic digestion of gliricidia leaves for biogas and organic manure[J].Biomass,1988,17(1):1-11.DOI:10.1016/0144-4565(88)90066-2.
[19] GUNASEELAN V N.Effect of inoculum/substrate ratio and pretreatments on methane yield from parthenium[J].Biomass and Bioenergy,1995,8(1):39-44.DOI:10.1016/0961-9534(94)00086-9.
[20] 邱凌,卢旭珍,王兰英,等.日光温室生产废弃物厌氧发酵特性初探[J].中国沼气,2005,23(2):30-32.DOI:10.3969/j.issn.1000-1166.2005.02.008. QIU Ling,LU Xuzhen,WANG Lanying,el al.Study on anaerobic digestion character of vegetable waste from greenhouse[J].China biogas,2005,23(2):30-32.DOI:10.3969/j.issn.1000-1166.2005.02.008.