Prokaryotic Expression and Antiserum Preparation of ALFFc in Chinese Shrimp (Fenneropenaeus chinensis)

doi:10.3969/j.issn.1000-1565.2010.01.019

Abstract

Abstract: The sequence coding mature peptide of anti-lipopolysaccharide factor(ALFFc)was cloned from Chinese shrimp Fenneropenaeus chinensis, and a prokaryotic expression vector pET-DsbA-ALFFc was constructed by PCR and recombinant DNA techniques. A high-productivity colony of transformed E. coli BL21(DE3) was obtained by IPTG screening method. The optimal conditions for the introduction of the fused protein were investigated, which are described as follows: final IPTG concentration is 0.8 mmol/L, initial OD_(600) is 0.7, and introduction is conducted at 37 ℃ for 3 h. The purified recombinant protein DsbA-ALFFc was used as antigen to prepare antiserum in New Zealand white rabbit, which provides the basis for further functional analysis of ALFFc.

Key words: Fenneropenaeus chinensis, ALFFc, prokaryotic expression, antiserum

CLC Number:

LIU Feng-song,LI Ting,WANG Xiao-fei,TANG Ting. Prokaryotic Expression and Antiserum Preparation of ALFFc in Chinese Shrimp (Fenneropenaeus chinensis)[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(1): 88-92.

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