Experiment research of photodynamic therapy of colon cancer with a novel photosensitizer galactose substituted phthalocyanines

doi:10.3969/j.issn.1000-1565.2017.02.012

Abstract

Abstract: To explore the effect and its related mechanism of new photosensitizers galactose substituted phthalocyanines(T1—T4)on the proliferation of colon cancer cell CT-26,consequently to provide experimental basis for the clinical photodynamic therapy of colon cancer,the water distribution coefficient of T1—T4 was detected to understand its water-soluble ability,the concentration and incubation time of T1—T4 were detected by fluorescence spectrophotometer to know their influence on CT-26 cell absorbing capacity,the cytotoxic effect of T1—T4 to CT-26 was detected by MTT the autophagy,apoptosis and necrosis of the colon cancer cells were detected through MDC staining and flow cytometry.It was found that the- water-soluble ability of T1—T4 enhanced,with the number of galactose substituents increasing.The photodynamic damage effect of T1—T4 to CT-26 was positively correlated with their concentration.The absorptive amount of CT-26 to T1—T3 increased with the concentration of incubation and with the extension of incubation time,but it can hardly absorb T4.Autophagy,apoptosis and necrosis of CT-26 can be induced by T2,and there is obvious dose-dependent effect.Through the above data, colon cancer cell absorbs more T1—T3 when the incubation concentration and incubation time increase.T2 mediated photodynamic therapy can obviously inhibit CT-26.The mechanism may be due to cell autophagy.

Key words: phthalocyanine, photodynamic therapy, autophagy, apoptosis

CLC Number:

X835

GE Weiying,ZHAO Zhanjuan,HONG Ge,ZHAO Jianxi. Experiment research of photodynamic therapy of colon cancer with a novel photosensitizer galactose substituted phthalocyanines[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(2): 180-186.

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