Screening of Bacillus strains producing β-glucosidase and study on hydrolysis of soybean isoflavone glycosides

doi:10.3969/j.issn.1000-1565.2017.06.010

Abstract

Abstract: In order to obtain Bacillus strains capable of efficiently hydrolyze soybean isoflavone glycosides,this study screened Bacillus strains producing β-glucosidase from fresh feces of animals with the help of Aesculin separation culture medium.Then the strains with relatively higher β-glucosidase activity were identified and the ability of hydrolyzing isoflavone glucosides were studied.The results showed that a strains of Bacillus R2-2 with relatively higher β-glucosidase activity was screened out.The identity of strains R2-2 was Bacillus amyloliquefaciens.Strain R2-2 showed rather high efficiency in hydrolyzing isoflavone glucosides.The daidzin hydrolysis rate reached 53.65%,and genistin hydrolysis rate was 48.80%.

Key words: β-glucosidase, Bacillus, screen, soybean isoflavone glycosides

CLC Number:

S816

LIU Hongli,GUO Xiaojun,DI Congying,GUO Wei,ZHU Baocheng. Screening of Bacillus strains producing β-glucosidase and study on hydrolysis of soybean isoflavone glycosides[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(6): 621-629.

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