Cloning and expression of β-1,4-endoglucanase gene of cellulose derading bacteria N2-10

doi:10.3969/j.issn.1000-1565.2018.04.010

Abstract

Abstract: To validate the mechanism of cellulose degradation by Bacillus subtilis N2-10, through homology comparison,the degenerate primers was designed and the β-1,4-endoglucanase gene of B. subtilis N2-10 was amplified. By useing bioinformatics tools in analyzing the sequences of genes and proteins and considering that the expression of the β-1,4-endoglucanase gene in E.coli, the result shows that, this gene is 1 500 bp, which could totally encodle 499 amino acids. This study also found that it had the typical structure of cellulose. SDS PAGE analysis found that the apparent molecular weight of the expression product was about 55 ku. Congo red staining analysis found that the expression product had the activity of cellulose.

Key words: cellulose derading bacteria, Bacillus subtilis N2-10, endoglucanase, clone, expression

CLC Number:

DI Congying, GUO Xiaojun, LIU Hongli,GUO Wei, ZHU Baocheng. Cloning and expression of β-1,4-endoglucanase gene of cellulose derading bacteria N2-10[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(4): 403-409.

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