A turn-on fluorescent probe for GSH

doi:10.3969/j.issn.1000-1565.2019.01.009

Abstract

Abstract: As we all known that homocysteine(Hcy), cysteine(Cys), and glutathione(GSH)are common biothiols which play a crucial role in the human body. The use of fluorescent probes for detection of biothiols has many advantages, such as high sensitivity, good selectivity, and short response times. Because of the similar chemical structure(containing active sulfhydryl groups)of these biothiols, the selective detection of them is a challenge. In this article, we designed a GSH fluorescent probe(2-methyl, 6-acryloyl quinoline)to distinguish Cys/Hcy. By testing the spectral properties of the probe, we found that the addition of GSH in aqueous solution containing the probe caused a significant change in the fluorescence and UV-visible spectra. After comparing with other analytes, we found that the probe had higher selectivity and sensitivity to GSH in H₂O. In addition, considering that the detection process is performed in the aqueous phase, so the probe has a potential application value in biological imaging.

Key words: fluorescent probe, quinoline, biothiol, fluorescence enhancement

CLC Number:

O316

MENG Meirong, YIN Caixia. A turn-on fluorescent probe for GSH[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(1): 49-55.

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