Effect of GmGSL7c in SMV infected soybean

doi:10.3969/j.issn.1000-1565.2021.01.007

Abstract

Abstract: This work centered on the key role of soybean callose synthase in the process of soybean mosaic virus infection. Twenty four callose synthase were identified in soybean by bioinformatics in this experiment. Glyma.08G308200(GmGSL7c)was selected for RT-qPCR to verify that the transcriptome data was consistent with the actual expression trend.TRV-VIGS followed by callose fluorescence observation found that silencing GmGSL7c affected the synthesis of callose in the leaves, and the SMV-CP gene could be detected in the upper leaves of the silenced plants that were not inoculated. In addition, disease symptoms of SMV were observed in the GmGSL7c silenced plants, indicating that the silencing of GmGSL7c promotes the transportation of SMV in soybeans. This study lays the foundation for further research on the soybean-SMV interaction signal pathway for disease resistance.

Key words: nitric oxide, Soybean mosaic virus, callose, callose synthase

CLC Number:

Q291

WANG Mengxuan, SUN Xizhe, SUN Tianjie, ZHANG Jie, WANG Dongmei. Effect of GmGSL7c in SMV infected soybean[J]. Journal of Hebei University(Natural Science Edition), 2021, 41(1): 47-54.

References

[1] LIAO L, CHEN P, BUSS G R, et al. Inheritance and allelism of resistance to soybean mosaic virus in Zao18 soybean from China[J]. Journal of Heredity, 2002, 93(6): 447-452. DOI:10.1093/jhered/93.6.447.
[2] 杨洪强,接玉玲.植物钙素吸收和运转[J].植物生理与分子生物学学报,2005, 31(3): 227-234.DOI:10.3321/j.issn:1671-3877.2005.03.002.
[3] 王大刚,李凯,智海剑.大豆抗大豆花叶病毒病基因研究进展[J].中国农业科学,2018, 51(16): 3040-3059. DOI: 10.3864/j.issn.0578-1752.2018.16.002.
[4] LEE J Y. New and old roles of plasmodesmata in immunity and parallels to tunneling nanotubes[J]. Plant Science, 2014, 221/222: 13-20. DOI:10.1016/j.plantsci.2014.01.006.
[5] GUSEMAN J M,LEE J S, BOGENSCHUTZ N L, et al. Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis CHORUS(GLUCAN SYNTHASE-LIKE 8)[J]. Development, 2010, 137(10):1731-1741. DOI:10.1242/dev.049197.
[6] VATÉN A, DETTMER J, WU S, et al. Callose biosynthesis regulates symplastic trafficking during root development[J]. Developmental Cell, 2011, 21(6): 1144-1155. DOI:10.1016/j.devcel.2011.10.006.
[7] SIVAGURU M, FUJIWARA T, ŠAMAJ J, et al. Aluminum-induced 1→3-β-d-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata. A new mechanism of aluminum toxicity in plants[J]. Plant Physiology, 2000, 124(3): 991-1006. DOI:10.1104/pp.124.3.991.
[8] DE STORME N, GEELEN D. Callose homeostasis at plasmodesmata: molecular regulators and developmental relevance[J]. Frontiers in Plant Science, 2014, 5:138. DOI:10.3389/fpls.2014.00138.
[9] CHEN X Y, KIM J Y. Callose synthesis in higher plants[J]. Plant Signaling & Behavior, 2009,4(6): 489-492. DOI:10.4161/psb.4.6.8359.
[10] HONG Z L, DELAUNEY A J, VERMA D P. A cell plate-specific callose synthase and its interaction with phragmoplastin[J]. Plant Cell, 2001, 13(4): 755-768. DOI:10.1105/tpc.13.4.755.
[11] XIE B, WANG X M, ZHU M S, et al. CalS7 encodes a callose synthase responsible for callose deposition in the phloem[J]. Plant Journal, 2011,65(1): 1-14. DOI:10.1111/j.1365-313x.2010.04399.x.
[12] XIAO D Q, DUAN X X, ZHANG M S, et al. Changes in nitric oxide levels and their relationship with callose deposition during the interaction between soybean and Soybean mosaic virus[J]. Plant Biology, 2018, 20(2): 318-326. DOI:10.1111/plb.12663.
[13] LI W L, ZHAO Y S, LIU C J, et al. Callose deposition at plasmodesmata is a critical factor in restricting the cell-to-cell movement of Soybean mosaic virus[J]. Plant Cell Reports, 2012, 31(5): 905-916. DOI:10.1007/s00299-011-1211-y.
[14] SENTHIL-KUMAR M, MYSORE K S. Tobacco rattle virus-based virus-induced gene silencing in Nicotiana benthamiana[J]. Nature Protocols, 2014, 9(7): 1549-1562. DOI:10.1038/nprot.2014.092.
[15] MA S H, NIU H W, LIU C J, et al. Expression stabilities of candidate reference genes for RT-qPCR under different stress conditions in soybean[J]. PLoS One, 2013, 8(10): e75271. DOI: 10.1371/journal.pone.0075271.
[16] LEVY A, ERLANGER M, ROSENTHAL M, et al. A plasmodesmata-associated β -1,3-glucanase in Arabidopsis[J]. The Plant Journal, 2007,49: 669-682. DOI:10.1111/j.1365-313X.2006.02986.x.
[17] MIO T, ADACHI-SHIMIZU M, TACHIBANA Y, et al. Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/FKS1 and its involvement in β-1,3-glucan synthesis[J]. Journal of Bacteriology, 1997, 179(13): 4096-4105. DOI:10.1128/jb.179.13.4096-4105.1997.
[18] OKADA H, ABE M, ASAKAWA-MINEMURA M, et al. Multiple functional domains of the yeast l,3-β-glucan synthase subunit Fks1p revealed by quantitative phenotypic analysis of temperature-sensitive mutants[J]. Genetics, 2010, 184(4):1013-1024. DOI:10.1534/genetics.109.109892.
[19] XIAO J, XIA H, YOSHINO-KOH K, et al. Structural characterization of the ATPase reaction cycle of endosomal AAA protein Vps4[J]. Journal of Molecular Biology, 2007, 347(3): 655-670. DOI:10.1016/j.jmb.2007.09.067. (