Abstract: In order to alleviate the non-point source pollution caused by a large amount of waste mushroom bran, to study the resource development of mushroom bran, using Auricularia auricula bran as substrate, the content of gibberellins and indoleacetic acid in A. auricula bran was determined by spectrophotometry.The results showed that the mass fraction of gibberellin was 45.52 μg/g, and the recovery rate was 98.28%; the mass fraction of indole acetic acid was 38.42 μg/g, and the recovery rate was 94.70%.Using A. auricula bran mixed with vegetable garden soil and sandy soil in different quality ratios to plant purple leaf lettuce, it was found that compared with the control, the use of a certain proportion of A. auricula bran can increase the germination rate and shorten the germination time; when the addition amount of A. auricula bran is 35%(mass fraction),the content of the maximum germination rate is 86.91% in purple leaf lettuce,the shortest average germination time is about 7 d, indicating that A. auricula bran has the value for resource development.

Key words: bran of Auricularia auricula, gibberellins, indoleacetic acid, spectrophotometer, purple leaf lettuce