Performance of the sediment microbial fuel cell with algal-bacterial cathode for simultaneous treatment of excess sludge and aquaculture wastewater

doi:10.3969/j.issn.1000-1565.2025.05.007

Abstract

Abstract: To achieve efficient and simultaneous treatment and resource utilization of excess sludge and aquaculture wastewater, a sediment microbial fuel cell with algal cathode-sediment microbial fuel cell(AC-SMFC)was constructed by combining algal-bacterial symbiosis technology with sediment microbial fuel cell. A bacterial cathode-sediment microbial fuel cell(BC-SMFC)with activated sludge inoculated on the cathode was used as a control. The power generation performance, removal efficiencies of carbon, nitrogen and phosphorus, and the reduction effect of excess sludge in AC-SMFC were investigated.Results showed that the cathode potential and maximum output power density of AC-SMFC were(283.5±15.3)mV and 91.18 mW/m², respectively. After 41 days of operation, the removal rates of chemical oxygen demand(COD), ammonia nitrogen, total nitrogen and total phosphorus in aquaculture wastewater by AC-SMFC reached- DOI:10.3969/j.issn.1000-1565.2025.05.007菌藻阴极SMFC同步处理剩余污泥及养殖废水效能研究丁陈玉,杜旺,李永琦,陈利争,张勇泉,王洪杰,李慧(河北大学生态环境系,河北省湿地近自然修复技术重点实验室,河北保定 071002)摘要:为了实现剩余污泥和养殖废水的高效同步处理与资源化,将菌藻共生技术与沉积式微生物燃料电池结合构建了菌藻阴极型沉积式微生物燃料电池(algal cathode-sediment microbial fuel cell,AC-SMFC). 以阴极接种活性污泥的沉积式微生物燃料电池(baterial cathode-sediment microbial fuel cell,BC-SMFC)为对照,研究了AC-SMFC同步处理剩余污泥和养殖废水过程中的产电性能、阴极碳氮磷污染物去除效率及剩余污泥减量效果.结果表明,以剩余污泥和养殖废水分别作为底泥和上覆水时,AC-SMFC的最大输出功率密度和阴极电势分别为91.18 mW/m²和(283.5±15.3)mV.运行41 d后,AC-SMFC对养殖废水中化学需氧量、氨氮、总氮和总磷的去除率分别达到98.51%、81.63%、74.29%和65.84%,氮磷去除效果显著提升.AC-SMFC污泥减量效果显著,总化学需氧量和挥发性悬浮固体去除率分别为59.8%和68.5%.此外,AC-SMFC可促进松散结合胞外聚合物和紧密结合胞外聚合物中溶解性微生物产物、芳香族蛋白质和腐殖酸类物质的降解.关键词:养殖废水;降解;菌藻共生;微生物燃料电池;剩余污泥中图分类号:X703;TM911 文献标志码:A 文章编号:1000-1565(2025)05-0507-13Performance of the sediment microbial fuel cell with algal-bacterial cathode for simultaneous treatment of excess sludge and aquaculture wastewaterDING Chenyu, DU Wang, LI Yongqi, CHEN Lizheng, ZHANG Yongquan, WANG Hongjie, LI Hui(Hebei Key Laboratory of Close-to-Nature Restoration Technology of Wetlands, School of Eco-Environment, Hebei University, Baoding 071002, China)Abstract: To achieve efficient and simultaneous treatment and resource utilization of excess sludge and aquaculture wastewater, a sediment microbial fuel cell with algal cathode-sediment microbial fuel cell(AC-SMFC)was constructed by combining algal-bacterial symbiosis technology with sediment microbial fuel cell. A bacterial cathode-sediment microbial fuel cell(BC-SMFC)with activated sludge inoculated on the cathode was used as a control. The power generation performance, removal efficiencies of carbon, nitrogen and phosphorus, and the reduction effect of excess sludge in AC-SMFC were investigated.Results showed that the cathode potential and maximum output power density of AC-SMFC were(283.5±15.3)mV and 91.18 mW/m², respectively. After 41 days of operation, the removal rates of chemical oxygen demand(COD), ammonia nitrogen, total nitrogen and total phosphorus in aquaculture wastewater by AC-SMFC reached- 收稿日期:2024-12-10;修改稿日期:2025-01-09 基金项目:城市水资源与水环境国家重点实验室开放课题(HC202242);河北大学多学科交叉研究计划项目(DXK202106);河北省大学生创新创业训练计划项目(S202410075086);河北大学研究生创新项目(HBU2025SS023) 第一作者:丁陈玉(2001—),男,河北大学在读硕士研究生,主要从事生物电化学污水处理技术方向研究. E-mail:18655175391@163.com 通信作者:王洪杰(1972—),男,河北大学教授,博士生导师,主要从事生态修复技术原理与应用方向研究. E-mail:wanghj@hbu.edu.cn李慧(1987—),女,河北大学副教授,主要从事污水及污泥处理与资源化方向研究. E-mail:lihuihbu@hbu.edu.cn第5期丁陈玉等:菌藻阴极SMFC同步处理剩余污泥及养殖废水效能研究河北大学学报(自然科学版) 第45卷98.51%, 81.63%, 74.29% and 65.84%, respectively,indicating a significant improvement in nitrogen and phosphorus removal. AC-SMFC showed a significant sludge reduction effect, with the removal rates of total chemical oxygen demand(TCOD)and volatile suspended solids(VSS)reaching 59.8% and 68.5%, respectively. In addition, AC-SMFC could promote the degradation of soluble microbial products, aromatic proteins and humic substances in the loosely-bound extracellular polymeric substances(LB-EPS)and tightly-bound extracellular polymeric substances(TB-EPS).

Key words: aquaculture wastewater, degradation, symbiosis of bacteria and algae, microbial fuel cell, excess sludge

CLC Number:

X703
TM911

DING Chenyu, DU Wang, LI Yongqi, CHEN Lizheng, ZHANG Yongquan, WANG Hongjie, LI Hui. Performance of the sediment microbial fuel cell with algal-bacterial cathode for simultaneous treatment of excess sludge and aquaculture wastewater[J]. Journal of Hebei University(Natural Science Edition), 2025, 45(5): 507-519.

References

[1] 中华人民共和国住房和城乡建设部.中国城乡建设统计年鉴-2023[M].北京:中国城市出版社, 2024.
[2] YU S, CHEN Z P, LI M T, et al. Principles, challenges, and optimization of indigenous microalgae-bacteria consortium for sustainable swine wastewater treatment[J]. Bioresource Technol, 2024, 406: 131055.DOI:10.1016/j.biortech.2024.131055.
[3] MAHMOUDI A, MOUSAVI S A, DARVISHI P. Performance and recent development in sewage sludge-to-bioenergy using microbial fuel cells: A comprehensive review[J]. Int J Hydrog Energy, 2024, 50: 1432-1455. DOI:10.1016/j.ijhydene.2023.10.338.
[4] WANG W Y, WANG K, ZHAO Q L, et al. Maximizing electron flux, microbial diversity and gene abundance in MFC powered electro-Fenton system by optimizing co-addition of lysozyme and 2-bromoethanesulfonate[J]. J Environ Manag, 2022, 322: 116067. DOI:10.1016/j.jenvman.2022.116067.
[5] SUN F F, CHEN J F, SUN Z R, et al. Promoting bioremediation of brewery wastewater, production of bioelectricity and microbial community shift by sludge microbial fuel cells using biochar as anode[J]. Sci Total Environ, 2024, 929: 172418. DOI:10.1016/j.scitotenv.2024.172418.
[6] PENGADETH D, PRAKASH NAIK S, SASI A, et al. Revisiting the role of algal biocathodes in microbial fuel cells for bioremediation and value-addition[J]. Chem Eng J, 2024, 496: 154144. DOI:10.1016/j.cej.2024.154144.
[7] SHARMA M, JALALAH M, ALSAREII S A, et al. Microalgal cycling in the cathode of microbial fuel cells(MFCs)induced oxygen reduction reaction(ORR)and electricity: A biocatalytic process for clean energy[J]. Chem Eng J, 2024, 479: 147431. DOI:10.1016/j.cej.2023.147431.
[8] BHADRA S, NAYAK S, SEVDA S. Simultaneous organic wastewater treatment and bioelectricity production in a dual chamber microbial fuel cell with Scenedesmus obliquus biocathode[J]. Energy Convers Manag, 2024, 316: 118849. DOI:10.1016/j.enconman.2024.118849.
[9] 吴夏芫,周楚新,支银芳,等.微藻型微生物燃料电池的研究进展[J].环境科学与技术, 2012, 35(4): 82-86. DOI:10.3969/j.issn.1003-6504.2012.04.018.
[10] LI M, ZHOU M H, TIAN X Y, et al. Enhanced bioenergy recovery and nutrient removal from swine wastewater using an airlift-type photosynthetic microbial fuel cell[J]. Energy, 2021, 226: 120422. DOI:10.1016/j.energy.2021.120422
[11] ZHANG Y, ZHAO Y Y, ZHOU M H. A photosynthetic algal microbial fuel cell for treating swine wastewater[J]. Environ Sci Pollut Res Int, 2019, 26(6): 6182-6190. DOI:10.1007/s11356-018-3960-4.
[12] MOFIJUR M, RASUL M G, HASSAN N M S, et al. Recent development in the production of third generation biodiesel from microalgae[J]. Energy Procedia, 2019, 156: 53-58. DOI:10.1016/j.egypro.2018.11.088.
[13] TAN X B, LAM M K, UEMURA Y, et al. Cultivation of microalgae for biodiesel production: A review on upstream and downstream processing[J]. Chin J Chem Eng, 2018, 26(1): 17-30. DOI:10.1016/j.cjche.2017.08.010.
[14] KIM D W, SHIN W S, SUNG M G, et al. Light intensity control as a strategy to improve lipid productivity in Chlorella sp. HS2 for biodiesel production[J]. Biomass Bioener, 2019, 126: 211-219. DOI:10.1016/j.biombioe.2019.05.014
[15] 国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社, 2002.
[16] ZHANG R, FANG X, MA Y X, et al. Research of synergistic effect of magnetic powder and biosurfactant in pollutants removal and membrane fouling alleviation[J]. Sep Purif Technol, 2023, 326: 124855. DOI:10.1016/j.seppur.2023.124855.
[17] LOWRY O H, ROSEBROUGH N J, FARR L A, et al. Protein measurement with the folin phenol reagent[J]. J Biol Chem, 1951, 193(1): 265-275.
[18] SCARANO S, PASCALE E, MINUNNI M. The early nucleation stage of gold nanoparticles formation in solution as powerful tool for the colorimetric determination of reducing agents: The case of xylitol and total polyols in oral fluid[J]. Anal Chim Acta, 2017,993: 71-78. DOI:10.1016/j.aca.2017.09.020.
[19] SONG X S, WANG W T, CAO X, et al. Chlorella vulgaris on the cathode promoted the performance of sediment microbial fuel cells for electrogenesis and pollutant removal[J]. Sci Total Environ, 2020, 728: 138011. DOI:10.1016/j.scitotenv.2020.138011.
[20] NEETHU B, GHANGREKAR M M. Electricity generation through a photo sediment microbial fuel cell using algae at the cathode[J]. Water Sci Technol, 2017, 76(11/12): 3269-3277. DOI:10.2166/wst.2017.485.
[21] ABAZARIAN E, GHESHLAGHI R, MAHDAVI M A. Impact of light/dark cycle on electrical and electrochemical characteristics of algal cathode sediment microbial fuel cells[J]. J Power Sources, 2020, 475: 228686. DOI:10.1016/j.jpowsour.2020.228686.
[22] LING J Y, XU Y B, LU C S, et al. Enhancing stability of microalgae biocathode by a partially submerged carbon cloth electrode for bioenergy production from wastewater[J]. Energies, 2019, 12(17): 3229. DOI:10.3390/en12173229.
[23] CHEN S L, PATIL S A, SCHRÖDER U. A high-performance rotating graphite fiber brush air-cathode for microbial fuel cells[J]. Appl Energy, 2018, 211: 1089-1094. DOI:10.1016/j.apenergy.2017.12.013.
[24] HE Z, SHAO H B, ANGENENT L T. Increased power production from a sediment microbial fuel cell with a rotating cathode[J]. Biosens Bio electron, 2007, 22(12): 3252-3255. DOI:10.1016/j.bios.2007.01.010.
[25] ZHAO F, HARNISCH F, SCHRÖDER U, et al. Challenges and constraints of using oxygen cathodes in microbial fuel cells[J]. Environ Sci Technol, 2006, 40(17): 5193-5199. DOI:10.1021/es060332p.
[26] ROZENDAL R A, HAMELERS H V M, BUISMAN C J N. Effects of membrane cation transport on pH and microbial fuel cell performance[J]. Environ Sci Technol, 2006, 40(17): 5206-5211. DOI:10.1021/es060387r.
[27] 范晓萌.微生物燃料电池水凝胶电极制备及产电特性研究[D].济南:山东交通学院, 2023. DOI:10.27864/d.cnki.gsjtd.2023.000133.
[28] SRIKANTH S, VENKATA MOHAN S. Change in electrogenic activity of the microbial fuel cell(MFC)with the function of biocathode microenvironment as terminal electron accepting condition: Influence on overpotentials and bio-electro kinetics[J]. Bioresour Technol, 2012, 119: 241-251. DOI:10.1016/j.biortech.2012.05.097.
[29] LIU H Z, CHEN T Z, WANG N, et al. A new strategy for improving MFC power output by shared electrode MFC-MEC coupling[J]. Appl Energy, 2024, 359: 122677. DOI:10.1016/j.apenergy.2024.122677.
[30] JIANG N, SONG J L, YAN M Y, et al. Iron cobalt-doped carbon nanofibers anode to simultaneously boost bioelectrocatalysis and direct electron transfer in microbial fuel cells: Characterization, performance, and mechanism[J]. Bioresour Technol, 2023, 367: 128230. DOI:10.1016/j.biortech.2022.128230.
[31] KHAN M D, LI D, TABRAIZ S, et al. Integrated air cathode microbial fuel cell-aerobic bioreactor set-up for enhanced bioelectrodegradation of azo dye Acid Blue 29[J]. Sci Total Environ, 2021, 756: 143752. DOI:10.1016/j.scitotenv.2020.143752.
[32] SUN Y B, LI P R, HUANG Y, et al. Synergistic treatment of digested wastewater with high ammonia nitrogen concentration using straw and microalgae[J]. Bioresour Technol, 2024, 412: 131406. DOI:10.1016/j.biortech.2024.131406.
[33] ARUN S, SINHAROY A, PAKSHIRAJAN K, et al. Algae based microbial fuel cells for wastewater treatment and recovery of value-added products[J]. Renew Sustain Energy Rev, 2020, 132: 110041. DOI:10.1016/j.rser.2020.110041.
[34] MINUTILLO M, FLAGIELLO F, NASTRO R A, et al. Performance of two different types of cathodes in microbial fuel cells for power generation from renewable sources[J]. Energy Procedia, 2018, 148: 1129-1134. DOI:10.1016/j.egypro.2018.08.030.
[35] LIU H, FANG H H P. Extraction of extracellular polymeric substances(EPS)of sludges[J]. J Biotechnol, 2002, 95(3): 249-256. DOI:10.1016/S0168-1656(02)00025-1.
[36] RABAEY K, VERSTRAETE W. Microbial fuel cells: Novel biotechnology for energy generation[J]. Trends Biotechnol, 2005, 23(6): 291-298. DOI:10.1016/j.tibtech.2005.04.008.
[37] ZHANG X Q, BISHOP P L. Biodegradability of biofilm extracellular polymeric substances[J]. Chemosphere, 2003, 50(1): 63-69. DOI:10.1016/S0045-6535(02)00319-3.
[38] YU J L, XIAO K, XU H, et al. Spectroscopic fingerprints profiling the polysaccharide/protein/humic architecture of stratified extracellular polymeric substances(EPS)in activated sludge[J]. Water Res, 2023, 235: 119866. DOI:10.1016/j.watres.2023.119866
[39] WANG X B, ZHANG Y D, HE D, et al. Biodegradation of activated sludge extracellular polymeric substances as the electron donors for denitrification[J]. J Environ Chem Eng, 2024, 12(2): 112077. DOI:10.1016/j.jece.2024.112077.
[40] LI J P, CHEN Y X, QI J, et al. Characterization of EPS subfractions from a mixed culture predominated by partial-denitrification functional bacteria[J]. Water Res X, 2024, 24: 100250. DOI:10.1016/j.wroa.2024.100250.
[41] LI W, LI X, HAN C X, et al. A new view into three-dimensional excitation-emission matrix fluorescence spectroscopy for dissolved organic matter[J]. Sci Total Environ, 2023, 855: 158963. DOI:10.1016/j.scitotenv.2022.158963.
[42] 李艳,周鑫,平彩霞.连续低氧曝气与间歇曝气主流Anammox运行效能及微生物特性对比[J].中国环境科学, 2024, 44(9): 4910-4917. DOI:10.19674/j.cnki.issn1000-6923.2024.0171. (