Journal of Hebei University(Natural Science Edition) >

2016 , Vol. 36 >Issue 1: 78 - 83

DOI: https://doi.org/10.3969/j.issn.1000-1565.2016.01.013

Melatonin inhibited P-selectin expression on acute lung injury induced by lipopolysaccharide in rats

  • ZHANG Zhi ,
  • GU Weiwei ,
  • MA Yunlei ,
  • DONG Yujie ,
  • DING Chunhua
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  • 1.College of Basic Medical Science, Hebei University, Baoding 071000, China; 2.Experiment Center, The Third Hospital of Hebei Medical University, Shijiazhuang 050017, China; 3.Department of Respiration, Traditional Chinese Medicine Hospital of Hebei Province, Shijiazhuang 050017, China; 4.Department of Pathology, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, China; 5.Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China

Received date: 2015-03-12

  Online published: 2016-01-25

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Abstract

To observe the expression of P-selectin(Ps)in lung tissues of acute lung injury(ALI)rat model induced by lipopolysaccharide(LPS)and examine the effect of melatonin(MT)to its expression all rats were randomly divided into three groups:Control group,LPS group and MT+LPS group.Lung tissues were imbedded by paraffin to observe morphological changes.The auther used immunohistochemical staining technique,reverse transcription-polymerase chain reaction(RT-PCR)and western blot method to- 收稿日期:2015-03-12 基金项目:河北省科技厅资助项目(08276101D-88) 第一作者:张智(1982—),男,河北衡水人,河北大学实验师,主要从事病理学基础实验研究.E-mail:zhzh313@163.com 通信作者:丁春华(1954—),女,河北保定人,河北医科大学教授,主要从事急性肺损伤研究.E-mail:dch54@sina.comdetect the expression of P-selectin.The results showed that compared with Control group,alveolar septum thickened significantly in LPS group,positive expression of P-selectin was very obvious(P<0.05);the administration of MT mitigated above changed significantly(P<0.05).The results suggested that MT possessed obvious protective effect on lung tissues during ALI,its protective mechanism might be related to the inhibition of the expression of P-selectin.

Key words： melatonin; acute lung injury; Ps; LPS

Cite this article

ZHANG Zhi , GU Weiwei , MA Yunlei , DONG Yujie , DING Chunhua . Melatonin inhibited P-selectin expression on acute lung injury induced by lipopolysaccharide in rats[J]. Journal of Hebei University(Natural Science Edition), 2016 , 36(1) : 78 -83 . DOI: 10.3969/j.issn.1000-1565.2016.01.013

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