Journal of Hebei University(Natural Science Edition) >

2010 , Vol. 30 >Issue 2: 164 - 170

DOI: https://doi.org/10.3969/j.issn.1000-1565.2010.02.012

Fingerprint of Pilose Antler by HPLC

  • YAN Zheng ,
  • YU Chen ,
  • ZHANG Zhi-hui ,
  • CAI Li-peng ,
  • WANG Chun-yun ,
  • YUAN Ruo-yao ,
  • WANG Ji-kun
Expand

Online published: 2010-03-25

Fold

Abstract

This paper described an analytical method of fingerprint of pilose antler by HPLC. different sample pretreatment ,detection wavelength , mobile phase, temperature were studied and the fingerprint chromatograms of pilose antler from six different areas were compared. Inertsil ODS-3 column(250 mm×4.6 mm,5 μm,) was used with the mixture of methanol- phosphoric 0.05% acid;the detection wavelength was 270 nm;the flow rate was 1 mL/min and the column temperature was 25 ℃.

Key words： pilose antler; HPLC; fingerprint

Cite this article

YAN Zheng , YU Chen , ZHANG Zhi-hui , CAI Li-peng , WANG Chun-yun , YUAN Ruo-yao , WANG Ji-kun . Fingerprint of Pilose Antler by HPLC[J]. Journal of Hebei University(Natural Science Edition), 2010 , 30(2) : 164 -170 . DOI: 10.3969/j.issn.1000-1565.2010.02.012

References

[1] 于文静, 王焕群, Yu Wen-jing, Wang Huan-qun. HPLC法测定花鹿茸饮片中尿嘧啶及次黄嘌呤的含量 [J]. 中国药品标准 2006.doi:10.3969/j.issn.1009-3656.2006.05.022
[2] 梁志敏, 李建平, 林喆, 原忠. 不同产地鹿茸中次黄嘌呤的HPLC含量测定 [J]. 中药材 2005.doi:10.3321/j.issn:1001-4454.2005.08.017
[3] 安春志, 刘莉, 刑学锋, 罗佳波, AN Chun-zhi, LIU Li, XING Xue-feng, LUO Jia-bo. 中药指纹图谱的现状和研究进展 [J]. 第一军医大学分校学报 2005.
[4] 江苏新医学院. 中药大辞典 [M]. 上海:上海科学技术出版社 1977.
[5] 杨秀伟. 花鹿茸、马鹿茸碱基成分的HPLC定量分析和其MAO活性抑制作用 [J]. 中草药 1995, 26(01).
[6] 杨秀伟, 白云鹏. 马鹿茸化学成分的研究 [J]. 中草药 1994, 25(05).
[7] 黄晓巍, 于秀华, 付春. 鹿茸乙醇提取工艺的研究 [J]. 中国社区医师 2006, 8(146).
[8] 任伟, 邓晓健. 注意驯鹿角片混充鹿茸片 [J]. 中药材
Options
Outlines

/

〈 〉