Fingerprint of Pilose Antler by HPLC

doi:10.3969/j.issn.1000-1565.2010.02.012

Abstract

Abstract: This paper described an analytical method of fingerprint of pilose antler by HPLC. different sample pretreatment ,detection wavelength , mobile phase, temperature were studied and the fingerprint chromatograms of pilose antler from six different areas were compared. Inertsil ODS-3 column(250 mm×4.6 mm,5 μm,) was used with the mixture of methanol- phosphoric 0.05% acid;the detection wavelength was 270 nm;the flow rate was 1 mL/min and the column temperature was 25 ℃.

Key words: pilose antler, HPLC, fingerprint

CLC Number:

O657.7

YAN Zheng,YU Chen,ZHANG Zhi-hui,CAI Li-peng,WANG Chun-yun,YUAN Ruo-yao,WANG Ji-kun. Fingerprint of Pilose Antler by HPLC[J]. Journal of Hebei University (Natural Science Edition), 2010, 30(2): 164-170.

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Fingerprint of Pilose Antler by HPLC

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