Corn thick mash was used as main nitrogen source to produce Nisin.Firsty,the fermentation media were optimized by single factor experiment.The results indicated that the growth of Lactococcus lactis was obviously enhanced by adding sucrose,yeast extract and K2HPO4 to thick mash.As the starting strain,the strain was further treated by atmospheric and room temperature plasmas and quickly selected using 24 well culture plate.At a survival rate of 5%,the positive mutation rate of the Lactococcus lactis was found to be 26.2% evaluated by the comparison with the starting strain.The results from shake flask culture further confirmed that a positive mutant strains could produce 6 520 IU/mL Nisin.
ZHANG Shuanli
,
HU Shihai
,
CUI Yun
,
ZHAO Dishun
. Mutating Lactococcus lactis for high-yield strain of Nisin using corn thick mash as main nitrogen source[J]. Journal of Hebei University(Natural Science Edition), 2016
, 36(4)
: 417
-423
.
DOI: 10.3969/j.issn.1000-1565.2016.04.014
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