Mutating Lactococcus lactis for high-yield strain of Nisin using corn thick mash as main nitrogen source

doi:10.3969/j.issn.1000-1565.2016.04.014

Abstract

Abstract: Corn thick mash was used as main nitrogen source to produce Nisin.Firsty,the fermentation media were optimized by single factor experiment.The results indicated that the growth of Lactococcus lactis was obviously enhanced by adding sucrose,yeast extract and K₂HPO₄ to thick mash.As the starting strain,the strain was further treated by atmospheric and room temperature plasmas and quickly selected using 24 well culture plate.At a survival rate of 5%,the positive mutation rate of the Lactococcus lactis was found to be 26.2% evaluated by the comparison with the starting strain.The results from shake flask culture further confirmed that a positive mutant strains could produce 6 520 IU/mL Nisin.

Key words: Nisin, Lactococcus lactis, atmospheric and room temperature plasma(ARTP)mutation, corn thick mash

CLC Number:

Q815

ZHANG Shuanli,HU Shihai,CUI Yun,ZHAO Dishun. Mutating Lactococcus lactis for high-yield strain of Nisin using corn thick mash as main nitrogen source[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(4): 417-423.

References

[1] BREUKINK E,de KRUIJFF B. The lantibiotic Nisin,a special case or not[J].Biochimica et Biophysica Acta, 1999,1462:223-234.DOI:10.1016/S0005-2736(99)00208-4.
[2] GIFFARD C J,LADHA S,MACKIE A R,et al.Interaction of nisin with planar lipid bilayers monitored by fluorescence recovery after photobleaching[J].Membr Biol,1996,151:293-300.DOI:10.1007/s002329900079.
[3] PEREZ R H,ZENDO T,SONOMOTO K.Novel bacteriocins from lactic acid bacteria(LAB):various structures and applications[J].Microb Cell Fact,2014,13(Suppl 1):S3.DOI:10.1186/1475-2859-13-S1-S3.
[4] ISLAM M R,NAGAO J,ZENDO T,et al.Antimicrobial mechanism of lantibiotics[J].Biochem Soc Trans,2012,40(6):1528-1533.DOI:10.1042/BST20120190.
[5] 闫国栋,姜丽艳,王秀丽,等.利用浓醪酒糟生产乳链菌肽的发酵培养基优化[J].酿酒科技,2009,3:33-36. YAN G D,JIANG L Y,WANG X L,et al.Optimization of fermentation medium for Nisin production by thick mash[J].Liquor-making Science & Technology,2009,3:33-36.
[6] 王玉红,丁重阳,章克昌,等.以浓醪酒糟为基质的发酵生产单细胞蛋白的工艺条件的优化[J].酿酒,2002,29(5):44- 46. WANG Y H,DING C Y,ZHANG K C,et al.Study on the fermentation of SCP on the base of the distillerss grain[J].Liquor-making,2002,29(5):44-46.
[7] IBRAHIM RAJOKA M,TARIQ KIANI M A,SOHAIL KHAN,et al.Production of single cell protein from rice polishings using Candida utilis[J].World Journal of Microbiology & Biotechnology,2004,20:297- 301.DOI:10.1023/B:WIBI.0000023845.96123.dd.
[8] ZHANG Y F,LIU S Y,DU Y H,et al.Genome shuffling of Lactococcus lactis subspecies lactis YF11 for improving Nisin Z production and comparative analysis[J].Dairy Sci,2014,97(5):2528-2541.DOI:10.3168/jds.2013-7238.
[9] CHEIGH C I1,PARK H,CHOI H J,et al.Enhanced nisin production by increasing genes involved in Nisin Z biosynthesis in Lactococcus lactis ssp.lactis A164[J].Biotechnol Lett,2005,27:155-160.DOI:10.1007/s10529-004-7661-3.
[10] TODOROV S D,DICKS L M.Influence of growth conditions on the production of a bacteriocin by Lactococcus lactis subsp.lactis ST34BR,a strain isolated from barley beer[J].Basic Microbiol,2004,44(4):305-316.DOI:10.1002/jobm.200410413.
[11] TODOROV S D,VAN REENEN C A,DICKS L M.Optimization of bacteriocin production by Lactobacillus plantarum ST13BR,a strain isolated from barley beer[J].Gen Appl Microbiol,2004,50(3):149-157.
[12] CHEIGH C I,CHOI H J,PARK H,et al.Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp.Lactis A164 isolated from Kimchi[J].Biotechnol,2002,95(3):225-235.DOI:10.1016/S0168-1656(02)00010-X.
[13] WANG L Y,HUANG Z L,LI G,et al.Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma[J].Appl Microbiol,2010,108(3):851-858.DOI:10.1111/j.1365-2672.2009.04483.x.
[14] ZHANG X,ZHANG X F,LI H P,et al.Atmospheric and room temperature plasma(ARTP)as a new powerful mutagenesis tool[J].Appl Microbiol Biotechnol,2014,98(12):5387-5396.DOI:10.1007/s00253-014-5755-y.
[15] LAROUSSI M.Low temperature plasma-based sterilization:overview and state-of-the-art[J].Plasma Proc Polym,2005,2:391-400.DOI:10.1002/ppap.200400078.
[16] 万青,曹伟佳,张常青,等.常压室温等离子体诱变高效利用木糖产丁二酸菌株[J].生物工程学报,2013,29(11):1692-1695.DOI:10.13345/j.cjb.2013.11.017. WAN Q,CAO W J,ZHANG C Q,et al.Mutating Escherichia coli by atmospheric and room temperature plasmas for succinic acid production from xylose[J].Chinese Journal of Biotechnology,2013,29(11):1692-1695.DOI:10.13345/j.cjb.2013.11.017.
[17] 董晓宇,滕虎,修志龙.高产1,3-丙二醇的等离子体诱变菌株选育及发酵动力学分析[J].过程工程学报,2011,11(2):304-311. DONG X Y,TENG H,XIU Z L.Selection of strain exposed under plasma discharge with enhanced 1,3-propanediol production and kinetic analysis of batch fermentation[J].The Chinese Journal of Process Engineering,2011,11(2):304-311.
[18] QIAO M,OMAETXEBARRIA M J,Ra R, et al.Isolation of a Lactococcus lactis strain with high resistance to nisin and increased nisin production[J].Biotechnol Lett,1997,19(2):199-202.DOI:10.1023/A:1018384919362.