Table of Content

25 July 2016, Volume 36 Issue 4

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Stability and Hopf bifurcation analysis for a class of genetic regulator model

CAO Jianzhi,BAO Junyan,ZHOU Meng

2016, 36(4):  337-342.  DOI: 10.3969/j.issn.1000-1565.2016.04.001

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The main purpose of this paper is to investigate a class of genetic regulator model which was described by ordinary differential equations using center manifold theory and normal form theorem. Some sufficient conditions which guarantee the local stability and Hopf bifurcation are obtained,and some numerical simulation for justifying the theoretical results is also provided.

Mathematical foundation for interpreting chaos and ecological phenomenon with uniform index

DENG Qun,LUO Chuanwen

2016, 36(4):  343-348.  DOI: 10.3969/j.issn.1000-1565.2016.04.002

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The uniform index of arbitrary distribution function was first defined, then the axioms of ‘uniform index independence’ and proposition of ‘uniform distribution is the most uniform’ were put forward. The similarity between uniform index and entropy was clearly illustrated, and the inner mechanism of interpreting chaos with uniform index was revealed thereby. According to the independence axiom, k step expectation uniform index does exist, and can be estimated by k step average uniform index. As the arbitrary distribution function F can be regarded as a transformation of uniform distribution by F^-1, numerical calculation indicates the the nonlinear degree of distribution function is the direct cause for k step average uniform index reduction. Entropy is a varable to describe the uncertainty of distribution, meanwhile, it also describes the disperse degree of samples, which means the degree of uniformity and vice versa. Certainty of distribution signifies the concentration degree of samples, which also implies the non-uniformity and vice versa. This is the common ground of entropy and uniform index. In ecology, the fact of most structures are clustering is caused by the nonlinear transformation existing in the nature.

Variation of recurrent fractal interpolation surface

ZHANG Wenjing,FENG Zhigang

2016, 36(4):  349-352.  DOI: 10.3969/j.issn.1000-1565.2016.04.003

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To calculate the dimension of grap of function, the minimum boxes can be replaced by the variation of fractal interpolation function, and the box-dimension formula can be proved from another angle.Based on the study of the properties of variation of bivariate continuous function,the variation of recurrent fractal interpolation function is estimated on the rectangular domain,which provides a new method to study dimension of recurrent fractal graph.

Preparation and photocatalytic properties of the Cr-doped nano-TiO₂ emulsion

XIAO Junying,XU Jun,WU Chengjie,SUI Huidong,DING Wenge

2016, 36(4):  353-357.  DOI: 10.3969/j.issn.1000-1565.2016.04.004

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Cr-doped nano-TiO₂ colorless and transparent hydrosol was synthesized by a complexation controlled hydrolysis method at room temperature using TiCl₄,Cr(NO₃)₃·6H₂O,D-sorbitol and organic carboxylic acid as raw materials.The phase structure,composition,particle size,absorbance spectrum,and photo-catalysis of samples were characterized by XRD,nano laser particle size analyzer,and ultraviolet-visible spectrophotometer.The results indicated that the Cr-doped nano-TiO₂ transparent photocatalyst exhibits optimal photocatalytic performance when the doping content of the amount of ratio substance was 0.5% and the refluxing time was 15 min.The degradation efficiency of acid red 3R dye solution with a concentration- DOI:10.3969/j.issn.1000-1565.2016.04.004铬掺杂纳米二氧化钛乳液的制备及其光催化性能肖俊莹¹,许军²,武承杰³,眭慧东¹,丁文革¹(1.河北大学 物理科学与技术学院,河北 保定 071002;2.惠州商贸旅游高级职业技术学校 机电工程系,广东 惠州 056025;3.河北大学 电子信息工程学院, 河北 保定 071002)摘 要:以四氯化钛(TiCl₄)、硝酸铬(Cr(NO₃)₃·6H₂O)、D-山梨糖醇、有机羧酸等为主要原材料,采用新技术——络合-控制水解法,在室温下合成铬(Cr)掺杂纳米TiO₂无色透明乳液.分别用XRD、纳米激光粒度分析仪、和紫外-可见分光光度仪等,对样品的物相、粒径、组成、光吸收、光催化等性质进行了表征.结果显示,当Cr的掺杂物质的量比为0.5%,回流时间为15 min,pH为6时,所得样品Cr掺杂纳米TiO₂无色透明乳液的光催化性能最好;在光照50 min后,其对酸性红3R的降解率最大达96%.关键词:纳米TiO₂无色透明乳液;铬掺杂;络合控制水解法;光催化中图分类号:TQ613.5 文献标志码:A 【additional_page=368】文章编号:1000-1565(2016)04-0353-05Preparation and photocatalytic properties of the Cr-doped nano-TiO₂ emulsionXIAO Junying¹,XU Jun²,WU Chengjie³,SUI Huidong¹,DING Wenge¹(1.College of Physics Science and Technology,Hebei University,Baoding 071002,China;2.Department of Electrical and Mechanical Engineering Huizhou College of Business,Huizhou 056025,China;3. Electronic Information Engineering College, Hebei University, Baoding 071002,China)Abstract:Cr-doped nano-TiO₂ colorless and transparent hydrosol was synthesized by a complexation controlled hydrolysis method at room temperature using TiCl₄,Cr(NO₃)₃·6H₂O,D-sorbitol and organic carboxylic acid as raw materials.The phase structure,composition,particle size,absorbance spectrum,and photo-catalysis of samples were characterized by XRD,nano laser particle size analyzer,and ultraviolet-visible spectrophotometer.The results indicated that the Cr-doped nano-TiO₂ transparent photocatalyst exhibits optimal photocatalytic performance when the doping content of the amount of ratio substance was 0.5% and the refluxing time was 15 min.The degradation efficiency of acid red 3R dye solution with a concentration- 收稿日期:2015-11-04 基金项目:国家自然科学基金资助项目(21201053);高等学校博士学科点专项科研基金新教师类资助课题(20121301120005);河北省自然科学基金资助项目(F2014201078;A2015201050);河北省应用基础研究计划重点基础研究项目(14964306D);河北省教育厅基金资助项目(QN2014057;ZD2016055);河北省大学生创新创业训练计划项目(2015066;2015161;201510075047,026);河北大学学生综合素质培养项目(2015zh0442;2015zh0443);河北省研究生创新资助项目(S2016023);河北大学研究生创新资助项目(X2016065;X2016066);河北大学杰出青年基金资助项目(2015JQ02) 第一作者:肖俊莹(1990—),男,河北省承德人,满族,河北大学在读硕士研究生,主要从事新型纳米材料及光电器件的研究.E-mail:xiaojunyinghbu@163.com 通信作者:丁文革(1966—),女,河北枣强人,河北大学教授,主要从事新型纳米材料及光电器件研究.E-mail:dingwghbu@163.com第4期肖俊莹等:铬掺杂纳米二氧化钛乳液的制备及其光催化性能of 1 000 mg/L has reached more than 96% after exposed to sunlight for 50 min.

Device for surface treatment by plasma

ZHANG Hao,DONG Lifang,WANG Hao,GAO Xing

2016, 36(4):  358-361.  DOI: 10.3969/j.issn.1000-1565.2016.04.005

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A new-type of device that use dielectric barrier diasharge to generate plasma for surface treatment is designed.It is driven by an ac alternating(urrent)power supply,and a large number of plasma is produced due to four or even more times diasharge per half cycle of the driven voltage.In addition,the device generats plasma at the place that is close to the materials which need to be processed and has a high use ratio of plasma,thus showing great promise in industrial application.

Zero-index metamaterial based on double U-shaped structure

GUO Liang,HUANG Xiaojun,YANG Helin

2016, 36(4):  362-368.  DOI: 10.3969/j.issn.1000-1565.2016.04.006

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A structure of zero-index metamaterial composed of two anti-symmetrical U-shaped metal via holes and strip wires is proposed.The retrieved results indicate that the effective permittivity and permeability of the proposed structure is close to 0 at the frequency of 7.63 GHz when normal linearly polarized wave is incident directly,and the experimental results coincide with the simulation results.At the same time,by changing the via holes spacing and the feet length of U-shaped metal,we analyze the characteristics of the zero refractive index to study the adjustment of the frequency responses of zero refractive index point.We find that the response frequency of the zero refractive index will be changed by adjusting the geometric parameters of unit cell.The adjustable U-shaped zero-index material is simple and can be easily fabricated which can be utilized conveniently in the fields of microwave devices.

Preparation of Ni-La@mSiO₂ catalyst and its catalytic performance in benzophenone hydrogenation

NIU Libo,CHU Xiaoning,ZHANG Miao,CHU Hailong,BAI Guoyi

2016, 36(4):  369-373.  DOI: 10.3969/j.issn.1000-1565.2016.04.007

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The La modified Ni-La@mSiO₂ core-shell catalyst was prepared by hydration hydrazine reduction and Stöber method.The catalytic performance of the as-prepared catalyst was tested by hydrogenation of benzophenone to benzhydrol.BET,XRD and TEM were used to investigate the structure characteristics of the catalyst.The influence of the reaction time on the selective hydrogenation of benzophenone over Ni-La@mSiO₂ were investigated and optimized.The conversion of benzophenone and selectivity of benzhydrol were both over 90%,while the hydrogen pressure was 2.5 MPa,the reaction temperature was 120 ℃ and the reaction time was 120 min.Furthermore,there was no significant decline of the catalyst when it was recycled for five runs,indicating the good stability of this catalyst.

Optimization of traceability model of Ziziphus jujuba geographic origin by multi-element analysis combined SIMCA

XIA Liya,LI Xiaoting,LI Xiaoyang,ZHANG Xiaoyu,YIN Jiexuan

2016, 36(4):  374-379.  DOI: 10.3969/j.issn.1000-1565.2016.04.008

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In order to improve the accuracy of identification method of geographical origin,the contents of 10 elements in 69 Ziziphus jujubas were measured,and the data were analyzed by difference analysis,principal component analysis(PCA)and soft independent modeling of class analogies(SIMCA).The results showed that there were significant differences in the contents of Mg,B,Mn,Fe,Zn and other elements from different origin places.The result of PCA showed that the samples from different origins had clustering trend.In the first four principal components,the Fe,B,Mn,Zn and K elements had higher loading values,they were considered important origin elements.In the SICMA,with the 5% significance level,the identified result was best with 100% recognition rate and 78.95% rejection rate.This study confirmed that multi-element analysis with SICMA is on effective method to determine geographical origin of agricultural products.

Chemical composition of indoor dust during Haze weather by FTIR spectroscopy

SUN Ying,ZHANG Yanfen,MA Yan,QIN Xinying,MA Gang

2016, 36(4):  380-386.  DOI: 10.3969/j.issn.1000-1565.2016.04.009

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Haze weather is a serious threat to the peoples’ health in today’s China.As people tend to stay indoor during haze weather,the indoor air quality thus becomes a topic of great interest.In this study,we explore to use FTIR spectroscopy to investigate the chemical composition of indoor dust collected during haze weather.We collected indoor dust samples during haze weather in the city of Baoding which is the most air polluted city in China.Through IR spectral analysis,we get a general view towards the chemical composition of indoor dust.We found indoor dust contains inorganic and organic components.Inorganic components include sulfate,nitrate,phosphate,and ammonium salts;while organic components include proteins.We believe our findings are beneficial to a better understanding of the impact of haze weather on human health.

Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method

WANG Quan,LI Hongya,LI Shuna,WANG Hui,LI Moli

2016, 36(4):  387-395.  DOI: 10.3969/j.issn.1000-1565.2016.04.010

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A 17-run Box-Behnken design was used to optimize the extraction conditions of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method.Three factors,namely,extraction temperature,extraction time,and solid-liquid ratio were investigated.The experimental data were fitted to a second-order polynomial equation through multiple regression analysis and examined with the appropriate statistical methods.The adjusted coefficient of determination(R²_Adj)for the model was 0.815 3,and the probability value(P<0.000 1)demonstrated a high significance for the regression model.The optimum extraction conditions were as follows:extraction temperature of 60 ℃,extraction time of 52 min,and solid-liquid ratio of 1∶20.Under these conditions,the mean extraction yield of maca polysaccharides was 15.732%,which was in good agreement with the predicted value of 15.831%.Box-Behnken response surface method is used to optimize extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method,the optimized conditions are feasible,the model predition is good.

Microwave assisted synthesis and antibacterial study of 2-aryl-1,3-benzoxathiin-4-ones

FENG Junna,PENG Shaohui,CUI Penglei,CHEN Hua,LI Xiaoliu

2016, 36(4):  396-401.  DOI: 10.3969/j.issn.1000-1565.2016.04.011

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A series of 2-aryl-1,3-benzoxathiin-4-ones were synthesized by aromatic aldehyde and thiosalicylic acid under microwave irradiation for 5 min.The reaction is fast,simple and has high yield.The structures of the synthesized compounds were characterized by IR,NMR,MS spectra and elemental analysis.Antibacterial(Escherichia coli and Dysentery bacillus)activities of the compounds against Escherichia coli and Dysentery bacillus were evaluated.The results showed that 2-aryl-1,3-benzoxathiin-4-ones had good antibacterial activity.

Cloning and expression of Micrococcus luteus rpf gene and structure prediction of Rpf protein

ZHANG Xiumin,JING Fengxia,WANG Haiqiang,ZHAO Ruowei,ZHANG Yanfen

2016, 36(4):  402-411.  DOI: 10.3969/j.issn.1000-1565.2016.04.012

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In order to study the properties of resuscitation-promoting factor(Rpf)of Micrococcus luteus,rpf gene(813 pb)was amplified by using genome DNA of M. luteus ACCC41016 as templates.pET-28a(+)-rpf expression vector was constructed and Rpf protein was induced using IPTG to express in Escherichia coli BL21(DE3).Based on rpf nucleic acid sequence,the bioinformation of Rpf protein,such as protein amino acid sequence,hydrophobic,signal peptide,phosphorylation sites,tertiary structure,structure and functional domains,were predicted by using bioinformatics method.The results showed that the identity of nucleotide sequences between the cloning rpf gene and the reportorial rpf gene was 69.72%.And the identity of amino acids sequences between the protein encoded by the cloning rpf gene and the reportorial Rpf protein was 63.44%.SDS-PAGE results confirmed that the expression of rpf gene in the E.coli BL21 - DOI:10.3969/j.issn.1000-1565.2016.04.012藤黄微球菌rpf基因的克隆表达及其结构预测张秀敏¹,景凤霞¹,王海强¹,赵若玮¹,张艳芬²(1.河北大学 生命科学学院,河北 保定 071002;2.河北大学 科学技术处,河北 保定 071002)摘 要:为了了解藤黄微球菌(Micrococcus luteus)复苏促进因子(resuscitation-promoting factor,Rpf)的性质,用自行设计的引物,以藤黄微球菌ACCC41016基因组DNA为模板,扩增rpf基因.随后,构建pET-28a(+)-rpf表达载体,在Escherichia coli BL21(DE3)中进行IPTG诱导表达,并以克隆测得的rpf基因核酸序列为基础,对Rpf蛋白的氨基酸序列、疏水性、信号肽、磷酸化位点、三级结构以及结构功能域进行生物信息学预测分析.结果表明:该rpf基因与文献报道的藤黄微球菌IAM 14879 rpf基因核苷酸序列一致性为69.72%;该rpf基因所编码蛋白的氨基酸序列与文献报道的Rpf蛋白氨基酸序列一致性为63.44%.在E.coli BL21(DE3)中成功表达了Rpf目的蛋白.初步了解了Rpf蛋白的理化性质及生物学功能.解释了Rpf蛋白能够使细菌复苏并促进其生长的理论,为进一步探讨Rpf蛋白的作用机理奠定了基础.关键词:藤黄微球菌;复苏促进因子(Rpf);克隆;表达;结构预测中图分类号:Q939.13; 文献标志码:A 文章编号:1000-1565(2016)04-0402-10Cloning and expression of Micrococcus luteus rpf gene and structure prediction of Rpf proteinZHANG Xiumin¹,JING Fengxia¹,WANG Haiqiang¹,ZHAO Ruowei¹,ZHANG Yanfen²(1.College of Life Scineces,Hebei University,Baoding 071002,China;2.Office of Science and Technology,Hebei University,Baoding 071002,China)Abstract:In order to study the properties of resuscitation-promoting factor(Rpf)of Micrococcus luteus,rpf gene(813 pb)was amplified by using genome DNA of M. luteus ACCC41016 as templates.pET-28a(+)-rpf expression vector was constructed and Rpf protein was induced using IPTG to express in Escherichia coli BL21(DE3).Based on rpf nucleic acid sequence,the bioinformation of Rpf protein,such as protein amino acid sequence,hydrophobic,signal peptide,phosphorylation sites,tertiary structure,structure and functional domains,were predicted by using bioinformatics method.The results showed that the identity of nucleotide sequences between the cloning rpf gene and the reportorial rpf gene was 69.72%.And the identity of amino acids sequences between the protein encoded by the cloning rpf gene and the reportorial Rpf protein was 63.44%.SDS-PAGE results confirmed that the expression of rpf gene in the E.coli BL21 - 收稿日期:2016-04-13 基金项目:国家自然科学基金资助项目(31270053);河北省自然科学基金资助项目(C2014201141);河北省生物工程重点学科建设经费资助项目(1050-5030023);河北省高等学校科学技术研究资助项目(Z2015013) 第一作者:张秀敏(1970—),女,河北涞水人,河北大学教授,博士,主要从事微生物系统性及多样性研究.E-mail:zhxiumin1106@126.com 通信作者:张艳芬(1979—),女,河北邯郸人,河北大学讲师,主要从事分子生物学研究.E-mail:zhangjing@hbu.edu.cn第4期张秀敏等:藤黄微球菌rpf基因的克隆表达及其结构预测(DE3)was successful.According to the bioinformatics and homology analysis of Rpf protein,the physical and chemical properties and biological function of Rpf protein were preliminary elucidated.The study explained the theory of the Rpf protein can make the bacteria recovery and promote its growth.This laid the groundwork for further mechanistic studies of Rpf protein.

Cloning,expression,purification and crystallization of murein-tripeptide amidase MpaA from Escherichia coli O157

MA Yinliang,LIU Shuang,CUI Yaqi,KANG Xianjiang,LIU Xiuhua

2016, 36(4):  412-416.  DOI: 10.3969/j.issn.1000-1565.2016.04.013

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The gene mpaA from Escherichia coli O157 was inserted into pET-21b(+)vector using molecular cloning method.The recombinant protein MpaA was overexpressed in E.coli BL21(DE3)competent cells,and purified to homogeneity sequentially via a three-step protocol consisting of Ni-chelating affinity column,ion-exchange column and size-exclusion column chromatography.Crystallization search and optimization for MpaA were carried out using the sitting-drop vapour-diffusion method.The MpaA crystal diffracted to 0.26 nm resolution and belonged to the space group P31,with unit-cell parameters a=5.989 3 nm,b=5.989 3 nm,c=12.987 0 nm and β=90.000 °.Preliminary crystallographic analysis builds up a good foundation for the structural determination and catalytic mechanism of MpaA.

Mutating Lactococcus lactis for high-yield strain of Nisin using corn thick mash as main nitrogen source

ZHANG Shuanli,HU Shihai,CUI Yun,ZHAO Dishun

2016, 36(4):  417-423.  DOI: 10.3969/j.issn.1000-1565.2016.04.014

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Corn thick mash was used as main nitrogen source to produce Nisin.Firsty,the fermentation media were optimized by single factor experiment.The results indicated that the growth of Lactococcus lactis was obviously enhanced by adding sucrose,yeast extract and K₂HPO₄ to thick mash.As the starting strain,the strain was further treated by atmospheric and room temperature plasmas and quickly selected using 24 well culture plate.At a survival rate of 5%,the positive mutation rate of the Lactococcus lactis was found to be 26.2% evaluated by the comparison with the starting strain.The results from shake flask culture further confirmed that a positive mutant strains could produce 6 520 IU/mL Nisin.

CP-ASBE based data encryption mechanism for cloud storage

YANG Xiaohui,DING Wenqing

2016, 36(4):  424-431.  DOI: 10.3969/j.issn.1000-1565.2016.04.015

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Attribute set encryption mechanism relies on an authorization center to generate key,which may easily become a system security bottleneck problem.So an authorization set encryption mechanism based on multiple attribute authorized institution is proposed.It can improve the security of the secret key.The authorization center consists of many attribute authorized institutions.To increase attack difficulty,many AA participate the complete key calculation.Each AA is responsible for managing some of the attributes set.In this paper,file encryption,key calculation and file decryption have been analyzed in cloud environ- ment.Data encryption mechanism has been efficiently designed under the cloud storage environment.The safety and overhead problems in the mechanism are also considered.Experiments indicated that the proposed method is effective.

Field crop growth monitoring system based on message queue service

WANG Sile,LU Sukui,YANG Wenzhu,CHEN Liping,CHEN Xiangyang

2016, 36(4):  432-437.  DOI: 10.3969/j.issn.1000-1565.2016.04.016

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It is difficult to acquire the growth information of the crops in the far field.A growth monitoring system for the field crops was designed to solve the problem.The designed system adopted a hybrid architecture which combined the B/S and C/S access mode.It made use of various techniques including ActiveMQ message server and AJAX.The crop growth information can be obtained at fixed time or in real time,and can be presented in multiple ways.The system function can also be deployed dynamically.The results indicate that the presented system was comprehensive,consistent and workable,and it can satisfy all requirements of information acquisition from the filed crops.

A personalized recommendation algorithm based on conditional relative average entropy

WU Liu,CHEN Meng,SHI Yongge

2016, 36(4):  438-443.  DOI: 10.3969/j.issn.1000-1565.2016.04.017

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In order to improve the accuracy of recommendation algorithm,one personalized recommendation algorithm based on conditional relative average entropy is presented.First of all,through weighted CNM algorithm we construct complex network and excavate the network’s community structure. The result is regarded as the uncertain recommendation domain. Further more,conditional mutual information and conditional relative average entropy are used to determine the effective node ordering as input of K2 algorithm,which can improve the accuracy of Bayesian network construction,and then learn Bayesian network by K2 algorithm and analyze the consumer characteristics. We use the relationship between the commodity and the consumer characteristics to confirm the recommendation domain.Finally,the empirical analysis of the actual data of the telecom operators is carried out to verify the validity of the above algorithm.

Application of virtual simulation technology for assembly manipulator

HAO Lei,GAO Yuehua,LI Yapeng,WAN Zhenzhen

2016, 36(4):  444-448.  DOI: 10.3969/j.issn.1000-1565.2016.04.018

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A virtual simulation system which is combines electrical control and virtual reality technology is proposed.The system’s monitoring,debugging and staff training for visual simulation process of industrial control applications are realized.The control program obtained by software of Rockwell is running on PLC.3D virtual simulation model of the automatic assembly system of manipulator is structured by the RSTestStand.To reduce mechanical arm joint motion and force effectively,acceleration and speed reduction of the manipulator movement and optimization of the speed using cosine timing scheme in uniform conversion were performed.The experimental verification of motion visual effect and real-time monitoring curve on the object of virtual simulation is achieved.