Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method

doi:10.3969/j.issn.1000-1565.2016.04.010

Abstract

Abstract: A 17-run Box-Behnken design was used to optimize the extraction conditions of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method.Three factors,namely,extraction temperature,extraction time,and solid-liquid ratio were investigated.The experimental data were fitted to a second-order polynomial equation through multiple regression analysis and examined with the appropriate statistical methods.The adjusted coefficient of determination(R²_Adj)for the model was 0.815 3,and the probability value(P<0.000 1)demonstrated a high significance for the regression model.The optimum extraction conditions were as follows:extraction temperature of 60 ℃,extraction time of 52 min,and solid-liquid ratio of 1∶20.Under these conditions,the mean extraction yield of maca polysaccharides was 15.732%,which was in good agreement with the predicted value of 15.831%.Box-Behnken response surface method is used to optimize extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method,the optimized conditions are feasible,the model predition is good.

Key words: maca polysaccharide, box-Behnken design, ultrasonic extraction, aqueous two-phase

CLC Number:

O629

WANG Quan,LI Hongya,LI Shuna,WANG Hui,LI Moli. Box-Behnken design for optimized extraction of polysaccharides from maca via ultrasonic-assisted aqueous two-phase method[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(4): 387-395.

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