Table of Content

25 May 2016, Volume 36 Issue 3

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A fuzzy decision tree induction algorithm based on loose lower approximation

ZHANG Qunfeng

2016, 36(3):  225-228.  DOI: 10.3969/j.issn.1000-1565.2016.03.001

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To induce a fuzzy decision tree from a continuous decision table,a method based on loose lower approximation in fuzzy rough set theory is proposed.First,a fuzzy decision table is generated by clustering fuzzy similarity relations.Second,based on loose lower approximation,a measure of the importance of a condition attribute is introduced.Finally,using this measure as a criterion for selecting the expanding attribute,a fuzzy decision tree induction algorithm is proposed and an illustrative example is provided.

Relationship between A-harmonic equation and differential form

GU Zhihua,CHEN Yating

2016, 36(3):  229-231.  DOI: 10.3969/j.issn.1000-1565.2016.03.002

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A-Harmonic equation and differential forms are very important in electromagnetic and fluid mechanics,and their relationship is particularly important.This paper introduces two types of weighted differential forms,and proves the relationship between differential form and A-harmonic equation.

Gain characteristics of InAs/GaAs quantum dot lasers

WEI Yuxin,CHEN Ruili

2016, 36(3):  232-236.  DOI: 10.3969/j.issn.1000-1565.2016.03.003

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Due to the atom-like state densities,quantum-dot(QD)lasers have a number of unique optical and electronic properties.In this work,InAs/GaAs quantum dot semiconductor lasers are fabricated,and their mode gain spectra are measured by the Fourier series expansion method and the Hakki-Paoli method.Hakki-Paoli method is found to be influenced by the resolution of the measurement system,leading to underestimated gain.On the other hand, we found that Fourier Series Expansion method with a correction factor derived from the response function of the measurement system can be used to obtain gain spectrum with high accuracy.

Fabrication of hollow porous carbon nanofibers by coaxial electrospinning

YANG Zelin,ZHENG Haoran,ZHI Shenghui,YANG Yanxing,ZHANG Wenming

2016, 36(3):  237-241.  DOI: 10.3969/j.issn.1000-1565.2016.03.004

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Core/shell polyacrylonitrile(PAN)fibers were prepared by coaxial electrospinning using PMMA solution as the core,PAN solution as the shell,ZnO as the pore former.After calcination and acid treatment,the hollow porous carbon nanofibers were obtained.Morphologies of the fibers were characterized by transmission electron microscope(TEM); and the effect of solution concentration,voltage and flow rates on core and shell diameters were also investigated.The results showed that the optimized condition for the satisfactory hollow porous carbon nanofibers was 10% PMMA,10% PAN,6% zinc acetate,15 kV,1.0 mL/h inner rate and 1.5 mL/h outer rates.

Purification of total saponins from Coreopsis tinctoria Nutt.in Xinjiang by macroporous adsorptive resin

YAO Xincheng,ZHANG Ting,TANG Hui,CHEN Wen

2016, 36(3):  242-248.  DOI: 10.3969/j.issn.1000-1565.2016.03.005

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To investigate the purification process of total saponins from Coreopsis tinctoria Nutt.using macroporous adsorptive resin.The assay of total saponins was determined by UV-Vis spectrophotometry,and the resins were selected using the parameters,such as static adsorption capacity,desorption ratio,dynamic adsorption capacity,and as well as dynamic desorption ratio.The D-101 macroporous resin is suitable for purification of total saponins from Coreopsis tinctoria Nutt.according to its adsorption and desorption ability for total saponins.The total saponins content is(40.53±0.81)%(n=3)in total solids through D-101 macroporous resin purification.The D-101 macroporous resin can be used for purification of total saponins obtained from Coreopsis tinctoria Nutt.in Xinjiang.

Different proportion and concentration of Glycyrrhizic acid -18-epimers effect on P-gp function in Caco-2 cells

ZHAO Yanyan,SUN Dong,LIU Liyan,LIU Yafei,WANG Jing

2016, 36(3):  249-256.  DOI: 10.3969/j.issn.1000-1565.2016.03.006

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To evaluate the effect of glycyrrhizic acid 18 epimers,18α-glycyrrhizic acid(18α-Gly)and 18β-glycyrrhizic acid(18β-Gly)at different proportion and concentration on the P-glycoprotein(P-gp)activity in colon cancer Caco-2 cell to select their appropriate concentration and proportion in use.Making a cell model,selecting the total glycyrrhizic acid concentration of 1,10,30,60,120,240 μmol/L and proportion of 10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10,determining their optimum concentration and proportion by cell survival rate,and detecting the maximum fluorescence value by flow cytometer,that is,thestrongest inhibitory effect of P-gp and its weakest function.When the concentration of glycyrrhizic acid was 1 μmol/L,the fluorescence intensity became gradually weak and its induction fuction was strengthed gradually with the reduction in proportion of 18α-Gly.When the concentration of glycyrrhizic acid was 10 μmol/L and- DOI:10.3969/j.issn.1000-1565.2016.03.006甘草酸18位差向异构体不同配比和浓度对Caco-2细胞P-gp功能的影响 赵燕燕^1,2,孙东¹,刘丽艳³,柳亚飞¹,王静¹( 1.河北大学 化学与环境科学学院,河北 保定 071002;2.河北大学 药学院,河北 保定 071002;3.河北大学 医学实验中心,河北 保定 071000)摘 要:研究甘草酸18位差向异构体18α-甘草酸(18α-Gly)与18β-甘草酸(18β-Gly)不同配比和浓度对结肠癌(Caco-2)细胞P-糖蛋白(P-gp)功能的影响,选择最佳浓度比例.建立细胞模型,选择甘草酸总浓度为1∶10∶30∶60∶120∶240 μmol/L,18α-Gly与18β-Gly物质的量比分别为10∶0、8∶2、6∶4、5∶5、4∶6、2∶8、0∶10,根据细胞存活率,选择合适浓度比例,利用流式细胞仪测定细胞内荧光强度,寻找荧光强度最大的浓度比,即对P-gp抑制作用最强,P-gp功能最弱.研究结果表明甘草酸总浓度为1 μmol/L时,随着18α-Gly比例的减少,荧光强度逐渐减弱,P-gp诱导作用逐渐增加.甘草酸总浓度为10 μmol/L和60 μmol/L时,随着两者物质的量比的变化,荧光强度变化明显;当总浓度为10 μmol/L,n(18α-Gly)∶n(18β-Gly)=4∶6时,荧光强度较强,抑制作用明显;当总浓度为60 μmol/L,两者物质的量比为5∶5时荧光强度最强,抑制作用最强.关键词:18α-甘草酸;18β-甘草酸;P-糖蛋白功能;Caco-2细胞;不同配比/浓度中图分类号:R285.5 文献标志码:A 文章编号:1000-1565(2016)03-0249-08Different proportion and concentration of Glycyrrhizicacid -18-epimers effect on P-gp function in Caco-2 cellsZHAO Yanyan^1,2,SUN Dong¹,LIU Liyan³,LIU Yafei¹,WANG Jing¹(1.College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;2.College of Pharmaceutical Sciences,Hebei University,Baoding 071002,China;3.Experimental Center of Medicine,Hebei University,Baoding 071000,China)Abstract:To evaluate the effect of glycyrrhizic acid 18 epimers,18α-glycyrrhizic acid(18α-Gly)and 18β-glycyrrhizic acid(18β-Gly)at different proportion and concentration on the P-glycoprotein(P-gp)activity in colon cancer Caco-2 cell to select their appropriate concentration and proportion in use.Making a cell model,selecting the total glycyrrhizic acid concentration of 1,10,30,60,120,240 μmol/L and proportion of 10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10,determining their optimum concentration and proportion by cell survival rate,and detecting the maximum fluorescence value by flow cytometer,that is,thestrongest inhibitory effect of P-gp and its weakest function.When the concentration of glycyrrhizic acid was 1 μmol/L,the fluorescence intensity became gradually weak and its induction fuction was strengthed gradually with the reduction in proportion of 18α-Gly.When the concentration of glycyrrhizic acid was 10 μmol/L and- 收稿日期:2015-10-08 基金项目:河北省自然科学基金资助项目(H2013201203);河北大学医学学科专项资金建设项目(2014A1003) 第一作者:赵燕燕(1960—),女,天津人,河北大学教授,主要从事药学以及将现代分离技术用于临床、药学、食品、环境等方面的研究.E-mail:zhaoyany606@tom.com第3期赵燕燕等:甘草酸18位差向异构体不同配比和浓度对Caco-2细胞P-gp功能的影响60 μmol/L,the fluorescence intensity changed significantly.When the concentration of glycyrrhizic acid was 10 μmol/L and their proportion was n(18α-Gly)∶n(18β-Gly)=4∶6,the fluorescence intensity became stronger and its inhibitory effect was remarkable.When the concentration of glycyrrhizic acid was 60 μmol/L and their proportion was n(18α-Gly)∶n(18β-Gly)=5∶5,the fluorescence intensity was the highest,and the strongest inhibitory effect of P-gp appeared.

Determination of vanilla mandelic acid and homovanillic acid based on capillay electrophoresis combinated with electrochemiluminescence detection

YANG Yang,SUN Xuemei,LI Chuanlong

2016, 36(3):  257-263.  DOI: 10.3969/j.issn.1000-1565.2016.03.007

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Vanilla mandelic acid(VMA)and homovanillic acid(HVA)in the urine were detected by capillary electrophoresis with electrochemiluminescence detection(CE-ECL).The following conditions were suitable for the determination of VMA and HVA:running buffer,50 mmol/L NaH₂PO₄-Na₂HPO₄(pH=8.2);separation voltage,16 kV;detection potential,1.2 V(vs.saturated calomel electrode(SCE)).Under the optimum conditions of detection,satisfactory sensitivities(LOD of 5.0×10^-7 mol/L for VMA and 6.1×10^-7 mol/L for HVA at S/N=3)were obtained,the correlation coefficients of the method were 0.999 3 and 0.997 9,respectively.The determination of these metabolites in human urine was completed within 10 min without any interferences,the recoveries were VMA 98% and HVA 99%,the result obtained is satisfactory.

Inhibition of carbon steel corrosion in solution saturated with CO₂ by ginkgo leaves extract

SU Fang,GU Mingguang,ZHAO Jingmao

2016, 36(3):  264-268.  DOI: 10.3969/j.issn.1000-1565.2016.03.008

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The active ingredients of ginkgo leaves were extracted by ultrasonic-assisted extraction.The inhibition effect of ginkgo leaves extract(QLE)on carbon steel corrosion in NaCl solution saturated with CO₂ was studied.Experimental results showed that QLE is a good corrosion inhibitor with maximum inhibition efficiency up to 84.7% and to 94.7% when combined with thiourea.Polarization curves reveal that QLE combined with thiourea behaves like a mixed-type corrosion inhibitor.

Diffusion of nitrate nitrogen in sediment of stream segment of Fuyang River through Hengshui City

BIAN Wei,TIAN Zaifeng,LI Nan,WEI Quanwei,XU Yuanqiang

2016, 36(3):  269-277.  DOI: 10.3969/j.issn.1000-1565.2016.03.009

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To solve the internal pollution,sediment and overlying water from xiaocao River were analyzed.Xiaocao River is the stream segment of Fuyang River through Hengshui City.The release of nitrate nitrogen is studied by static simulation in the laboratory.The diffusion flux of nitrate nitrogen on the interface of sediment-water is calculated.The results show that the content of carbon,nitrogen and phosphorus gradually decrease with the increase of the sampling depth.The dissolved oxygen is very lower,which indicates the overlying water is in an anoxic condition.The organic pollution is serious.The nutrient concentrations are close to or even more than the limits of Ⅴ class surface water environmental quality standard.The river has high nutritional risk.Under different temperatures,the release of nitrate nitrogen in the surface sediments rises first and then falls,and the lower the temperature,the more serious the release.At the temperature of 5 ℃,the release of nitrate nitrogen in different depths of sediments increases first and then becomes- DOI:10.3969/j.issn.1000-1565.2016.03.009滏阳河衡水市区段沉积物硝酸盐氮的扩散规律边蔚,田在锋,李楠,魏全伟,徐元强(河北省环境科学研究院 水环境科学研究所,河北 石家庄 050037)摘 要:为解决内源负荷污染问题,对滏阳河衡水市区主城区小漕河沉积物和上覆水的性质进行了分析测试,采用实验室静态模拟法研究了硝酸盐氮的释放规律,并计算了其沉积物-水界面的扩散通量.结果表明:垂向沉积物中C、N、P营养盐随着深度的增加,含量逐渐降低.上覆水呈缺氧状态,有机污染严重,营养盐浓度接近甚至超过地表水环境质量标准Ⅴ类限值,富营养化风险高.不同温度下,表层沉积物硝酸盐的释放先上升再下降,且温度越低,越有利于其释放.温度为5 ℃时,不同深度沉积物硝酸盐的释放先增加后平缓再逐渐下降,且随着深度的增加,释放强度逐渐减弱.硝酸盐氮释放通量为-0.49 mg/(m²·d),总体表现为由上覆水向沉积物扩散.研究结果为滏阳河衡水市区段环境综合整治工程以及子牙河流域水污染防治与水质改善提供数据支持.关键词:滏阳河;沉积物;上覆水;内源污染;硝酸盐氮;扩散规律中图分类号:X522 文献标志码:A 文章编号:1000-1565(2016)03-0269-09Diffusion of nitrate nitrogen in sediment of stream segmentof Fuyang River through Hengshui CityBIAN Wei,TIAN Zaifeng,LI Nan,WEI Quanwei,XU Yuanqiang(Institute of Water Environmental Sciences,Hebei Provincial Academy of Environmental Sciences,Shijiazhuang 050037,China)Abstract:To solve the internal pollution,sediment and overlying water from xiaocao River were analyzed.Xiaocao River is the stream segment of Fuyang River through Hengshui City.The release of nitrate nitrogen is studied by static simulation in the laboratory.The diffusion flux of nitrate nitrogen on the interface of sediment-water is calculated.The results show that the content of carbon,nitrogen and phosphorus gradually decrease with the increase of the sampling depth.The dissolved oxygen is very lower,which indicates the overlying water is in an anoxic condition.The organic pollution is serious.The nutrient concentrations are close to or even more than the limits of Ⅴ class surface water environmental quality standard.The river has high nutritional risk.Under different temperatures,the release of nitrate nitrogen in the surface sediments rises first and then falls,and the lower the temperature,the more serious the release.At the temperature of 5 ℃,the release of nitrate nitrogen in different depths of sediments increases first and then becomes- 收稿日期:2015-08-25 基金项目:国家水体污染控制与治理科技重大专项(2012ZX07209-003) 第一作者:边蔚(1983-),女,河北石家庄人,河北省环境科学研究院工程师,博士,主要从事水环境保护方面的研究.E-mail:bianbianweiwei@126.com 通信作者:田在锋(1971-),男,河北邢台人,河北省环境科学研究院高级工程师,主要从事环境保护方面的研究.E-mail:tianzaifeng@sina.com第3期边 蔚等:滏阳河衡水市区段沉积物硝酸盐氮的扩散规律stable and eventually gradually decrease,and when the depth is deeper,the release intensity is weaker.The amount of the NO₃^--N diffusion flux is -0.49 mg/(m²·d),and its releasing trend is from the overlying water to the sediment.The results provide data support to the environmental comprehensive renovation project of stream segment of Fuyang River through Hengshui City and to the water pollution control and water quality improvement of Ziya River basin.

Dynamics of phytoplankton community in Baiyangdian Lake based on the redundancy analysis(RDA)

LIU Cunqi,KONG Xiangling,ZHANG Zhirong,TIAN Zhifu

2016, 36(3):  278-285.  DOI: 10.3969/j.issn.1000-1565.2016.03.010

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The composition and temporal-spatial dynamics of phytoplankton community in Baiyangdian Lake were investigated from April to November of 2010,and main environmental factors affecting composition of community were analyzed by Redundancy analysis(RDA).Results showed that 183 species(genera)including 8 phyla of phytoplankton were identified,and the dominant phyla were found to be the Cyanophyta,Chlorophyta and Bacillariophyta.Phytoplankton density ranged from 8.68×10⁶ to 314.32×10⁶ /L,and the highest density was observed in autumn(September)and the lowest in winter(November).Shannon-Wiener biodiversity index and evenness in Caiputai were highest,but that in Wangjiazhai and Nanliuzhuang were lower.Water temperature,transparency and COD_Mn had strong effects on the community of phytoplankton,pH and total phosphorus correlated with species of Cyanophyta and Chlorophyta.To control the enviromental release of organic pollutants and phosphorus is the key approach to reduce the eutrophication risk in Baiyangdian Lake.

Molecular identification of crab spiders from Xiaowutai Mountains,China with DNA barcoding

HE Jingchao,HU Lanlan,GUO Chenhui,ZHANG Feng

2016, 36(3):  286-292.  DOI: 10.3969/j.issn.1000-1565.2016.03.011

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In order to explore the feasibility of DNA barcoding in the species identification of crab spiders,the COI gene of 110 thomisid samples belonged to 25 species in Xiaowutai Mountains,China,was applied; the phylogenetic trees were reconstructed by neighbor-joining(NJ)and Bayesian Inference(BI)methods,and with ABGD(automatic barcode gap discovery)software to divide the samples.The results showed that the samples belonging to the same species clustered in one monophyly and all species were successfully distinguished by the phylogenetic trees,and the groups automatically defined by ABGD perfectly matched the groups defined by morphological characters,and accorded with phylogenetic trees.We believe DNA barcoding can be used as an effective tool in the molecular identification of crab spiders.

Cloning and expression of human RACK1 gene

WANG Xiangfei, TANG Jingzhen, SHAN Jinshuai, LIU Chang, WU Chen

2016, 36(3):  293-299.  DOI: 10.3969/j.issn.1000-1565.2016.03.012

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Human RACK1 gene was cloned and constructed to pET-30a(+)prokaryotic expression vector.RACK1 protein was expressed and purified and identified by Western blot.A pair of primers was designed according to the digestion sites in pET-30a(+)and the RACK1 gene sequence published on GeneBank.RACK1 cDNA was amplified from a human fetal liver cDNA library and constructed into pET-30a(+)vector.The recombinant expression vector was transformed into the host E.coli DH5α.The positive clone was identified by double enzyme digestion and sequencing analysis.The recombinant expression vector pET-30a(+)-RACK1 was highly expressed in E.coli BL21(DE3)under the induction of IPTG.The fusion protein was analyzed by SDS-PAGE and purified by 6×His-Tagged Protein Purification Kit.The purified RACK1 protein was confirmed by Western blot.The results showed that the RACK1 cDNA sequence we cloned was right.The recombinant expression vector pET-30a(+)-RACK1 was successfully constructed.The fusion protein was expressed as inclusion body, which was purified by affinity chromatography.The result of Western blot indicated that the purified RACK1 protein could bind its specific antibody.The obtained human RACK1 protein laid a good foundation for further study on RACK1.- DOI:10.3969/j.issn.1000-1565.2016.03.012人RACK1基因的克隆及原核表达王向飞,汤静珍,单金帅,刘畅,吴琛(河北大学 生命科学学院,河北 保定 071002)摘 要:克隆人RACK1基因,构建原核表达载体pET-30a(+)-RACK1,诱导表达重组蛋白,纯化后Western blot鉴定目的蛋白.根据GeneBank提供的RACK1基因序列及pET-30a(+)载体上的多克隆位点设计引物,以人胎肝cDNA文库为模板,钓取RACK1 cDNA全长序列.将目的片段与原核表达载体pET-30a(+)连接,转入E.coli DH5α感受态细胞,筛选阳性克隆.将测序正确的重组质粒转入E.coli BL21(DE3)细胞,IPTG诱导表达,利用镍柱对表达的蛋白进行纯化,Western blot法检测纯化的蛋白.结果显示,克隆得到的目的基因序列与GeneBank中已报道的序列完全一致,成功构建了pET-30a(+)-RACK1原核表达载体.重组蛋白主要以包涵体形式存在,经镍离子亲和层析柱纯化后,Western blot检测证实纯化的蛋白能与特异性的抗体发生反应.本研究获得了人RACK1蛋白,为深入研究RACK1的功能奠定基础.关键词:人RACK1;基因克隆;原核表达;蛋白纯化中图分类号:Q78 文献标志码:A 文章编号:1000-1565(2016)03-0293-07Cloning and expression of human RACK1 geneWANG Xiangfei, TANG Jingzhen, SHAN Jinshuai, LIU Chang, WU Chen(College of Life Science, Hebei University, Baoding 071002, China)Abstract:Human RACK1 gene was cloned and constructed to pET-30a(+)prokaryotic expression vector.RACK1 protein was expressed and purified and identified by Western blot.A pair of primers was designed according to the digestion sites in pET-30a(+)and the RACK1 gene sequence published on GeneBank.RACK1 cDNA was amplified from a human fetal liver cDNA library and constructed into pET-30a(+)vector.The recombinant expression vector was transformed into the host E.coli DH5α.The positive clone was identified by double enzyme digestion and sequencing analysis.The recombinant expression vector pET-30a(+)-RACK1 was highly expressed in E.coli BL21(DE3)under the induction of IPTG.The fusion protein was analyzed by SDS-PAGE and purified by 6×His-Tagged Protein Purification Kit.The purified RACK1 protein was confirmed by Western blot.The results showed that the RACK1 cDNA sequence we cloned was right.The recombinant expression vector pET-30a(+)-RACK1 was successfully constructed.The fusion protein was expressed as inclusion body, which was purified by affinity chromatography.The result of Western blot indicated that the purified RACK1 protein could bind its specific antibody.The obtained human RACK1 protein laid a good foundation for further study on RACK1.- 收稿日期:2015-10-23 基金项目:国家自然科学基金资助项目(30800180);河北省自然科学基金资助项目(C2009000191; C2013201112);河北省教育厅优秀青年基金(Y2012024) 第一作者:王向飞(1989-),女,河南洛阳人,河北大学在读硕士研究生.E-mail:18330249598@163.com 通信作者:吴琛(1977-),女,河北保定人,河北大学教授,主要从事肿瘤分子生物学研究.E-mail:dawnwuchen@163.com第3期王向飞等:人RACK1基因的克隆及原核表达

Courtship behaviour ethogram and PAE coding system of captive black stork

ZHANG Zhenqun,GU Dehai,WANG Jiaojiao,HOU Jianhua,LIU Jianping

2016, 36(3):  300-306.  DOI: 10.3969/j.issn.1000-1565.2016.03.013

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The behaviours of five pairs of captive black stork(Ciconia nigra)were studied in the Baoding zoo from January to April of 2015 to obtain the PAE action coding system of Ciconia nigra courtship behaviour ethogram.The video data of the behaviour of five pairs of black stork was obtained by all occurrence methods,and the courtship behaviour from the video data was analysed and screened out second by second.Then the courtship behaviour was coded into PAE courtship behaviour ethogram.The results show that the courtship behaviour of male include 10 types of posture,42 types of actions,11 types of behaviours,and the PAE action coding system which contains the above mentioned posture code,action code and environment code was obtained.According to the form of courtship behaviour,the courtship behaviour was divided into song courtship behavior and action courtship behaviour.The song courtship behaviour include raising beak to sing and singing face to face.Action courtship behaviour include pecking grass to nesting,biting the grass into the nest,biting the grass out of the nest,staying in the nest by prone position,standing against each other and turning around,preening for each other,chasing,crossing the neck,leaneding forward to close to the beak.Among these behaviours,the eight- DOI:10.3969/j.issn.1000-1565.2016.03.013笼养黑鹳求偶行为谱及其PAE编码张振群¹,谷德海¹,王姣姣¹,侯建华¹,刘建平²(1.河北大学 生命科学学院 河北 保定 071002;2.河北农业大学 动物科技学院,河北 保定 071001)摘 要:2015年1-4月,为获得黑鹳(Ciconia nigra)求偶行为谱,对河北保定动物园内的5对黑鹳进行了行为学研究.采用全事件记录法获取每对黑鹳的行为视频资料,通过逐秒分析的方法筛选出求偶行为并编订PAE求偶行为谱.结果发现:雄鹳求偶行为包含10种姿势、42种动作、11种行为,并获得了包含这些行为的PAE行为谱.依据求偶行为发生的方式将求偶行为划分为鸣声求偶行为、动作求偶行为.鸣声求偶行为包含扬喙鸣叫、面面对鸣.动作求偶行为包含啄草理巢、衔草入巢、啄草出巢、俯身卧巢、对立转圈、理配偶羽、追逐、交颈、俯身近喙;其中,面面对鸣、啄草理巢、衔草入巢、啄草出巢、俯身卧巢、对立转圈、追逐、俯身近喙8种行为为新纪录求偶行为.关键词:黑鹳;求偶行为谱;PAE编码;笼养中图分类号:Q959.7 文献标志码:A 文章编号:1000-1565(2016)03-0300-07Courtship behaviour ethogram and PAE codingsystem of captive black storkZHANG Zhenqun¹,GU Dehai¹,WANG Jiaojiao¹,HOU Jianhua¹,LIU Jianping²(1.College of Life Sciences,Hebei University,Baoding 071002,China;2.College of Animal Science and Technology,Agricultural University of Hebei,Baoding 071002,China )Abstract:The behaviours of five pairs of captive black stork(Ciconia nigra)were studied in the Baoding zoo from January to April of 2015 to obtain the PAE action coding system of Ciconia nigra courtship behaviour ethogram.The video data of the behaviour of five pairs of black stork was obtained by all occurrence methods,and the courtship behaviour from the video data was analysed and screened out second by second.Then the courtship behaviour was coded into PAE courtship behaviour ethogram.The results show that the courtship behaviour of male include 10 types of posture,42 types of actions,11 types of behaviours,and the PAE action coding system which contains the above mentioned posture code,action code and environment code was obtained.According to the form of courtship behaviour,the courtship behaviour was divided into song courtship behavior and action courtship behaviour.The song courtship behaviour include raising beak to sing and singing face to face.Action courtship behaviour include pecking grass to nesting,biting the grass into the nest,biting the grass out of the nest,staying in the nest by prone position,standing against each other and turning around,preening for each other,chasing,crossing the neck,leaneding forward to close to the beak.Among these behaviours,the eight- 收稿日期:2015-08-25 基金项目:河北省自然科学基金资助项目(C2012204087);河北大学自然科学研究计划项目(2014-299) 第一作者:张振群(1989-),男,河北承德人,河北大学在读硕士研究生.E-mail:1121954214@qq.com 通信作者:侯建华(1968-),男,河北保定人,河北大学教授,博士,主要从事动物生态学研究.E-mail:13633325718@163.com第3期张振群等:笼养黑鹳求偶行为谱及其PAE编码behaviours of singing face to face,pecking grass to nesting,biting the grass into the nest,biting the grass out of the nest,staying in the nest by prone position,standing against and turning around,chasing and leaneding forward to close to the beak are new records of courtship behaviour.

Optimization SVR fog prediction model based on genetic algorithm

ZONG Xiaoping,WU Zihan,LIU Yan

2016, 36(3):  307-311.  DOI: 10.3969/j.issn.1000-1565.2016.03.014

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To perform smog forecast,this paper puts forward a model,which is based on support vector regression(SVR)method and GA method.The first eigenvector dimension is reduced using factor analysis,and the SVR parameters are optimized through the GA method,and put into the SVR model for the prediction of the PM2.5 value of the city of Baoding.Through comparison, an optimized prediction model is obtained.

Hierarchical visualization of CT angiography based on confidence-connected

WANG Guanglei,SHI Yingjie,YUAN Hao,WANG Hongrui,LIU Xiuling

2016, 36(3):  312-317.  DOI: 10.3969/j.issn.1000-1565.2016.03.015

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In order to achieve the hierarchical visualization of the human spine and blood vessels by using the CTA data,the recognition of the typical organization can be realized by properly vtilizing the intensity distribution of different organizations.By tacking the key problem of organization gray level overlapping,the separation of important organizations blood vessels and bone can be realized by three-dimensional region growing algorithm based on confidence-connected.Finally,the three-dimensional reconstruction is performed by volume rendering.The hierarchical visualization of the abdominal skin,muscle,bone and blood vessels is realized,and the structure can be displayed separately,can be freely rotated and zoomed in and out.By adjusting the parameters and three-dimensional segmentation, hierarchical visualization can be easily achieved.So it can provide a more clear and intuitive three-dimensional model for the doctor.

Energy recovery circuit based on NMOS transistor cross coupling logic

SHI Jianying,XU Yanbin,LIU Lei,MEN Jinxi

2016, 36(3):  327-331.  DOI: 10.3969/j.issn.1000-1565.2016.03.017

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The energy recovery circuit based on NMOS(N-Metal Oxide Semiconductor)-transistor cross coupling logic(NCCL)is studied.PMOS(P-Metal Oxide Semiconductor)transistors are used as the input tube to reduce the leakage current on the oxide gate layer in nanometer CMOS process.NCCL JK flip-flop is constructed by these techniques.The circuits are simulated by HSPICE in 90 nm CMOS BSIM3 process,and the simulation results show that NCCL inventor’s operation frequency can reach to 1 GHz. Compared with the ECRL(Efficient Charge Recovery Logic)inventor,when load capacity or clock frequency or power supply changes,the power consumptions of NCCL inventor decline to different degrees.In the same operate condition,the power consumption of NCCL JK flip-flop is about 50% of the ECRL JK flip-flop.

Thresholds of regular LDPC codes based on density evolution algorithm

YIN Xiaoqi

2016, 36(3):  332-336.  DOI: 10.3969/j.issn.1000-1565.2016.03.018

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To deal with the noise thresholds of LDPC codes,the density evolutionary algorithm based on belief propagation algorithm is discussed to determine the thresholds through simplifying the message iterative arithmetic on the means of the check nodes which can reduce the computing complexity and the amount of computation.Three different degree distribution LDPC codes have been constructed,and the theoretical calculation and simulation have been carried on to determine the noise variance thresholds and the SNR thresholds under AWGN channel,the results have been compared.It shows that with the increase of the column weight,the performance of LDPC codes becomes worse.Excellent performance can be obtained if we choose good numbers of degree distribution,that has guiding significance for further application research of LDPC codes.