Table of Content

25 March 2016, Volume 36 Issue 2

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Bounds on the rate of uniform convergence of learning process based on Hybrid samples

LI Junhua,BAI Heju

2016, 36(2):  113-116.  DOI: 10.3969/j.issn.1000-1565.2016.02.001

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Bounds on the rate of uniform convergence of learning process are important component part of statistical learning theory and the bounds determine the generalization abilities of learning machines.Based on the chance theory and the definition of Hybrid variable,bounds on the rate of uniform convergence of learning process based on hybrid samples are discussed and the relationship between the bounds and the capacity of the set of functions is given.

High accuracy explicit compact difference scheme for the diffusion equation

YANG Xiaojia,WANG Yan

2016, 36(2):  117-123.  DOI: 10.3969/j.issn.1000-1565.2016.02.002

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Based on the fourth-order compact difference formula of the second-order derivative in spatial direction and Taylor series expansion in time direction,a high-order explicit compact difference scheme for the one dimentional diffusion equation is developed.The stability of the scheme is analyzed by Fourier method,and the condition of stability is λ≤1/2(λ is the mesh ratio).Numerical experiments are carried out to verify the accuracy and reliability of the present scheme.

Preparation and photocatalysis performance of the sulfur-doped nano-TiO₂ transparent hydrosol

WANG Mingkun,TAI Youyi,CUI Tong,LI Xiaowei,LI Ling

2016, 36(2):  124-128.  DOI: 10.3969/j.issn.1000-1565.2016.02.003

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Sulfur-doped nano TiO₂ transparent hydrosol was synthesized by using TiCl₄,thiourea,organic carboxylic acid,NH₃·H₂O,D-sorbitol etc.as raw materials,through a novel complexation-controlled hydrolysis method at room temperature and atmospheric pressure.The hydrosol average particle size is 4.1 nm.The phase structure,particle size,composition,absorbance spectrum,and photocatalytic performance of samples were characterized by XRD,nano laser particle size analyzer and ultraviolet-visible spectrophotometer.Furthermore,the effect of reaction conditions on the synthesis process was also studied.The results show that the photocatalytic performance of sulfur-doped TiO₂ hydrosol was optimal when the doping content of S was 0.5% and the reflux time was 15 min.

Gradient strontium-brushite coatings of medical titanium alloy surface

LI Xinchang,YAN Yuting,FANG Qian,LIANG Yongqiang

2016, 36(2):  129-133.  DOI: 10.3969/j.issn.1000-1565.2016.02.004

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The concentration gradient of strontium-doped brushite coatings was prepared by two steps electrochemical depositions on the surface of medical titanium alloy(Ti-6Al-4V),which provided valuable theoretical reference for the clinical application and further experimental research of titanium(alloy)coating.The chemical compositions of coatings was analyzed by X-ray diffractometer(XRD),and the concentration of doped strontium was examed by X-ray fluorescence spectrometer(XRF).Field emission scanning electron microscopy(SEM)was used to examine the morphology of strontium-doped brushite coatings.The biocompatibility of strontium-doped brushite coatings was judged preliminarily by hemolysis rate that was tested by hemolysis test in vitro.The concentration of strontium was calculated according to the ratio of n(Sr)/n(Sr+Ga).Our results showed the concentrations of strontium-doped for strontium-doped brushite coatings were the percent of 10,15 and 20.The SEM images showed that the morphology of coatings was regular and compact.The rate of hemolysis was less than 5%,which was in accordance with the requirements of medical materials(<5%).

Synthesis and characterization of water-soluble fluorescent perylene bisimides

GAO Wei,XI Ya'nan,BO Fuli,YANG Tao,GAO Baoxiang

2016, 36(2):  134-140.  DOI: 10.3969/j.issn.1000-1565.2016.02.005

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Functional dye(PSPBI)was synthesized by the attachment of PEG and thioanisole groups to the imides and the bay of perylene bisimides,respectively.PSPBI dye was fully characterized by ¹H NMR spectroscopy,¹³C NMR spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry.The solubility of PSPBI dye in water is more than 10 mg/mL,indicating good water solubility.In aqueous solution,the absorption maximum of PSPBI dye appeared at 540 nm,along with two higher vibronic transitions located at 480 nm,and the emission peak located at 620 nm.When the concentration of hydrogen peroxide was increased,the absorption maximum and emission peak were blue-shifted by 29 nm and 15 nm,respectively.Furthermore,PSPBI dye showed a fluorescence increase of about 50 fold.

Preparation of silica encapsulated waterborne aluminum pigments based on nickel ammonia complex ion as anchoring agent

MA Zhiling,QIAO Yanjun,WANG Xianling,JIANG Zhen,ZHANG Chenguang

2016, 36(2):  141-147.  DOI: 10.3969/j.issn.1000-1565.2016.02.006

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The silica encapsulated waterborne aluminum pigments were prepared using［Ni(NH₃)₆］²⁺ as anchoring agent.Fourier transform infrared spectroscopy and X-ray diffraction results showed that the boehmite was formed on the surface of aluminum flakes by［Ni(NH₃)₆］²⁺ oxidizing aluminum.The results of scanning electron microscopy,optical microscopy and corrosion resistance test proved that by controlling the pH of encapsulated media at about 9.5,the boehmite on aluminum flakes surface can react with silanols which come from the hydrolysis of tetraethoxysilane and silane. This makes a silica sol-gel film form on the aluminum flakes surface.This sol-gel film improves the corrosion resistance of the aluminum pigments.

Synthesis and cytotoxicity of indeno［1,2-b］quinoxalin-11-one oxime ethers

FENG Weipei, WANG Yingxing, LIU Siyuan, WANG Shuxiang, LI Shenghui

2016, 36(2):  148-154.  DOI: 10.3969/j.issn.1000-1565.2016.02.007

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Ten novel indeno［1,2-b］quinoxalin-11-one oxime ethers(4A-4J)were synthesized by indeno［1,2-b］quinoxalin-11-one oxime, saturated dihalide, and piperidine or pyrrole, morpholine, and dibutylamine. Their cytotoxicity against HeLa, A549 and MCF-7 cell lines was evaluated by MTT assay. The results indicated that compounds 4A,4B,4E,4F, and 4J showed higher cytotoxicty than cisplatin against HeLa, A549, and MCF-7 cell lines. Among them, 4A, 4B and 4F exhibited 2 to 6 folds higher cytotoxicty than cisplatin. Both the length of oxime ether chain and the nature of the terminal group have important effects on the cytotoxicity. The cell cycle and apoptosis assays indicated that 4A could change the cells cycle ratio, and induce apoptosis. Our results suggest that indeno［1,2-b］quinoxalin-11-one oxime ether modified by pyrrole is a potential anti tumor precursor compound.

Sensitive detection of telomerase activity based on isothermal exponetial amplification reaction

ZHOU Xiaoyu,ZHANG Jiayu,ZHOU Man,JIA Hongxia

2016, 36(2):  155-161.  DOI: 10.3969/j.issn.1000-1565.2016.02.008

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Telomerase elongated product has many repeat sequences-(ggttag)_n.A specific DNA probe-(ctaacc)₂ which is matched with two repeat sequences is used to hybridize with telomerase elongated product.Excess DNA probes are attached to magnetic graphene and removed by magnetic separation.The DNA probes hybridized with telomerase elongated product is rapidly amplified by isothermal exponential amplification reaction(IEXPAR).SYBR Green I is utilized for real-time fluorescent detection of IEXPAR products.Under the optimal conditions,the telomerase activity in only 50 cancer cells can be easily detected.Sensitive detection of telomerase activity under isothermal condition is achieved.

Effects of atrazine on the enzymatic activities of LDH and ACP in spermatozoa of Eriocheir sinensis

MU Shumei,FAN Gaojun,ZHANG Caida,WANG Hailing,KANG Xianjiang

2016, 36(2):  162-168.  DOI: 10.3969/j.issn.1000-1565.2016.02.009

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In order to evaluate the reproductive toxicities of atrazine on male Chinese mitten handed crab,Eriocheir sinensis,mature male crabs were treated with 0.001,0.01,0.1,1 mg/L of atrazine for 2 weeks.The enzymatic activities of lactate dehydrogenase(LDH)and acid phosphatase(ACP)in the spermatozoa of the crabs were measured by spectrophotometry and polyacrylamide gel electrophresis.As for 0.1 mg/L atrazine groups,the activities of LDH and ACP were the highest and they were also significantly higher than the control groups(P<0.05).There was no significant difference in the activities of LDH and ACP between the control and the atrazine treated groups(0.001,0.01,1 mg/L;P>0.05).The changes of the activities of LDH and ACP after atrazine treatment detected by polyacrylamide gel electrophresis and spectrophotometry were consistent with each other.The results indicate that atrazine exposure affects the reproductivity of male E.sinensis.Our study can provide reference data for atrazine impact on human reproductive health.- 收稿日期:2015-07-10 基金项目:国家自然科学基金资助项目(31202000,31272309);河北省自然科学基金资助项目(C2011201028);河北大学博士基金资助项目(2012-239);河北省重点实验室项目(14967611D);河北省生物学强势特色学科建设项目;河北大学中西部高校提升综合实力工程项目 第一作者:穆淑梅(1972—),女,河北曲阳人,河北大学副教授,主要从事生殖毒理学研究.E-mail:shumeimu@126.com 通信作者:康现江(1964—),男,河北邯郸人,河北大学教授,博士生导师,主要从事细胞发育分化及其调控方面的研究.E-mail:xjkang@hbu.edu.cn第2期穆淑梅等:莠去津对中华绒螯蟹精子LDH、ACP活性的影响河北大学学报(自然科学版) 第36卷

High-throughput extraction and establishment of sample bank of the genome-wide DNA of different Chinese ethnic minorities

ZHANG Yiming,WANG Junli,HUANG Jingjing,ZHANG Rongrong,PENG Yao

2016, 36(2):  169-174.  DOI: 10.3969/j.issn.1000-1565.2016.02.010

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To preserve genetic resources of different Chinese ethnic groups’ genomes and establish the sample bank of the genome-wide DNA,2 mL peripheral blood was collected from each volunteer of different ethnic minorities according to the clinical blood collection standards and complying with the principle of informed consent.Automatic nucleic acid extraction machine was used to conduct high-throughput extraction of all blood samples,and the samples were numbered and put in the bank after passing quality control.The established sample bank of the genome-wide DNA includes 545 samples from ethnic minorities.The bank will provide material basis for the research of anthropogenesis and interaction of environment and genetic background;it will also serve as a resource for disease treatment and diagnostics.

Two new species of the genus Ascelosodis (Coleoptera,Tenebrionidae)from Chinese Himalayas

SHAN Junsheng,REN Guodong

2016, 36(2):  175-179.  DOI: 10.3969/j.issn.1000-1565.2016.02.011

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Two new species of the genus Ascelosodis Redtenbacher,1868,A.rectus sp.nov.and A.nyalamensis sp.nov.,are described and illustrated from the Chinese Himalayas,and new faunistic data for 27 known species are added.Ascelosodis rectus sp.nov.(Figs.1,2)The new species resembles Ascelosodis schmidi Kaszab,1965,with the following differences:anterior margin of clypeal lobe weakly sinuate;genae gradually wider before eyes;pronotum with anterior angles obtuse,rounded apically and with anterior margin shortly prominent in the middle;the fifth abdomen extending the end straight.Etymology:Named after the fifth abdomen extending the end straight.Holotype:♂,China,Tibet,Lhazê,Cuola Mountain,28°53.959'N,87°25.182'E;alt.5 064 m,2 August 2011,collected by Ren Guodong.Paratypes:16♂27♀,same data as the holotype.Ascelosodis nyalamensis sp.nov.(Figs.3,4)The new species resembles A.emarginata Ren & Shi,2006,with the following differences:Sides of prothorax and elytra with very sparsely ciliate beneath;pronotal surface with coarse punctures,slightly denser on disc than on sides and rugae between disc and sides;elytra base with obscure but finely entire- 收稿日期:2015-07-25 基金项目:国家自然科学基金资助项目(31572309) 第一作者:单军生(1988—),男,河北石家庄人,河北大学在读硕士研究生.E-mail:junshengshan@163.com 通信作者:任国栋(1955—),男,宁夏中宁人,河北大学教授,博士生导师,主要从事鞘翅目异跗节类的系统分类研究.E-mail:gdren@hbu.edu.cn第2期单军生等:中国喜马拉雅山宽额甲属2新种记述(鞘翅目:拟步甲科)河北大学学报(自然科学版) 第36卷 bordered;Elytra surface with sparsely and obscurely shallow punctures.Etymology:Named after the type locality.Holotype:♂,China,Tibet,Nyalam,Mainpu,Tulong,28°25.841'N,86°08.840'E,Alt.4 478 m,30 July 2014,collected by Ren Guodong.Paratypes:1♂3♀,collected by Ren Guodong,Bai Xinglong and Shan Junsheng,same data as the holotype.

Optimization of the cultivation medium for Lentinus edodes based on persimmon wood chips

LIU Min,QI Yuege,SHEN Xiaoyan,XU Xiaochen,WANG Qian

2016, 36(2):  180-183.  DOI: 10.3969/j.issn.1000-1565.2016.02.012

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Taking persimmon wood chips as materials,the formula of cultivation medium was studied according to the mycelial growth rate and biological efficiency.The results showed that the optimal cultivation medium was B.The mass fraction of each component was:persimmon wood chips 60%,sawdust 20%,bran 19%,and quicklime 1%.

Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro

LI Kuihua,LI Lu,YAN Peng,TONG Xiaobo,XIE Lide

2016, 36(2):  184-188.  DOI: 10.3969/j.issn.1000-1565.2016.02.013

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The effects of different concentrations of uric acid on hemorheology were investigated in vitro.The erythrocytes were suspended in 300,600,1 200 μmol/L uric acid for 2 h and the hemorheological parameters were measured.The membrane protein composition was also analyzed by SDS-PAGE.We found that the integrated deformation index and relax index were reduced in a dose-dependent manner.The hemolysis rate increased.As compared with the control group,the content of ankyrin and band 3 proteins showed a decrease trend.These results were statistically significant in the 1 200 μmol/L group.The results suggest that high concentrations of uric acid can have significant damaging effects on erythrocyte membrane proteins,which is an independent factor affecting the hemorheological properties of erythrocytes.

A micro-blogging community discovery method based on user’s interactions

XU Jianmin,LI Tengfei,WU Shufang

2016, 36(2):  189-196.  DOI: 10.3969/j.issn.1000-1565.2016.02.014

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By investigating the structure of micro-blogging and user’s communication mode,a micro-blogging community discovery method based on user’s interactions was presented.User’s interactions in micro-blogging are analyzed by referring to author cross citation and author coupling citation in citation analysis.Considering different interactions reflect different interests in micro-blogging articles,this paper sets different weights for different interactions and proposes a new calculation method of user’s similarity based on interactions that is used to divide micro-blogging community.Experimental results show that the proposed method can effectively perform community discovery.

Adaptive control based on switched model of electric machine with transition load

ZONG Xiaoping,CUI Enchang,WANG Xia,WANG Peiguang

2016, 36(2):  197-203.  DOI: 10.3969/j.issn.1000-1565.2016.02.015

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An adaptive tracking controller is designd for motor with shifting load in this paper.First,amathematic model of motor switching system is built where shifting load is regarded as a switching signal.Then adaptive controller of switching system is designed,which solves the problem of tracking error that increases rapidly after load switching and ensures asymptotic tracking of the system.Finally,examples and computer simulation validate the performance of designed controller,and the impact of switching signal on system dynamic property is explained.

Variable scale face recognition based on DCT and PCA

ZHANG Xin,LIU Hai,SONG Zhuozhan

2016, 36(2):  204-209.  DOI: 10.3969/j.issn.1000-1565.2016.02.016

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A variable scale face images recognition method is proposed, based on the DCT binding the PCA. First, different scale face images are transformed by DCT. Then, the same DCT coefficients are extracted;and the dimension of the DCT coefficients is reduced by PCA. Finally, matching and recognition of images is done. By the ORL and collected face images, it is proved that the method of recognition variable scale images is efficient. Effective values of DCT transformed coefficients and PCA feature dimension are measured.

A method for license plate recognition in vehicle based on Gabor feature and synergetic neural network

SHI Guimin,YU Wensen,XIAO Zhongjie

2016, 36(2):  210-217.  DOI: 10.3969/j.issn.1000-1565.2016.02.017

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Vehicle license recognition has been studied under the climate and light conditions.The feature extraction of Gabor and synergetic neural network has been used to enhance the recognition rate.First,the characters of vehicle license go through the binarization and segmenting to extract the characteristic parameters of the license characters by Gabor filter.Then,the synergetic mode is used to train the characteristic parameters and work out the training sample.Finally,the synergetic neural network is utilized to recognize the license character.Under a variety of environments and light conditions, this approach achieves a much higher rate of recognition rate compared with the traditional mode suggesting that this new method is very effective in the field of vehicle license recognition.

Design of FPGA-based SATA host controller in the link layer delivery

ZHANG Qingshun,LIU Zan,GUO Baozeng,ZHANG Suoliang

2016, 36(2):  218-224.  DOI: 10.3969/j.issn.1000-1565.2016.02.018

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SATA(serial advanced technology attachment)3.0 disk as a data storage medium cannot be directly used in electronic systems represented by FPGA(Field-programmable gate array).Referring to ATA1-7,SATA serial advanced technology attachment 1.0,SATA2.0,SATA3.0 and other relavant protocols,this paper proposes a design and implementation method originated from SATA3.0 host controller which is based on FPGA in the link layer of sending module.This method is successfully implemented in sending data validation,scrambling and flowing control.Simulation results are also provided.The results show that the data processing speed of the controller is 600 MB/s which verify the success of the design.