Effects of atrazine on the enzymatic activities of LDH and ACP in spermatozoa of Eriocheir sinensis

doi:10.3969/j.issn.1000-1565.2016.02.009

Abstract

Abstract: In order to evaluate the reproductive toxicities of atrazine on male Chinese mitten handed crab,Eriocheir sinensis,mature male crabs were treated with 0.001,0.01,0.1,1 mg/L of atrazine for 2 weeks.The enzymatic activities of lactate dehydrogenase(LDH)and acid phosphatase(ACP)in the spermatozoa of the crabs were measured by spectrophotometry and polyacrylamide gel electrophresis.As for 0.1 mg/L atrazine groups,the activities of LDH and ACP were the highest and they were also significantly higher than the control groups(P<0.05).There was no significant difference in the activities of LDH and ACP between the control and the atrazine treated groups(0.001,0.01,1 mg/L;P>0.05).The changes of the activities of LDH and ACP after atrazine treatment detected by polyacrylamide gel electrophresis and spectrophotometry were consistent with each other.The results indicate that atrazine exposure affects the reproductivity of male E.sinensis.Our study can provide reference data for atrazine impact on human reproductive health.- 收稿日期:2015-07-10 基金项目:国家自然科学基金资助项目(31202000,31272309);河北省自然科学基金资助项目(C2011201028);河北大学博士基金资助项目(2012-239);河北省重点实验室项目(14967611D);河北省生物学强势特色学科建设项目;河北大学中西部高校提升综合实力工程项目第一作者:穆淑梅(1972—),女,河北曲阳人,河北大学副教授,主要从事生殖毒理学研究.E-mail:shumeimu@126.com 通信作者:康现江(1964—),男,河北邯郸人,河北大学教授,博士生导师,主要从事细胞发育分化及其调控方面的研究.E-mail:xjkang@hbu.edu.cn第2期穆淑梅等:莠去津对中华绒螯蟹精子LDH、ACP活性的影响河北大学学报(自然科学版) 第36卷

Key words: atrazine, Eriocheir sinensis, spermatozoa, lactate dehydrogenase(LDH), acid phosphatase(ACP)

CLC Number:

MU Shumei,FAN Gaojun,ZHANG Caida,WANG Hailing,KANG Xianjiang. Effects of atrazine on the enzymatic activities of LDH and ACP in spermatozoa of Eriocheir sinensis[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(2): 162-168.

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