Distribution of peanut agglutinin receptors in spermatogenic cells of Eriocheir sinensis

doi:10.3969/j.issn.1000-1565.2019.03.010

Abstract

Abstract: In order to detect the distribution characteristics of peanut agglutinin(PNA)receptors in spermatogenic germ cells of Eriocheir sinensis, lectin-blotting and lectin-histochemistry were used in this study. The results showed that the molecular weight of PNA receptors was 72 ku. Based on the binding sites of the biotin-labeled PNA, positively stained cells could be observed and recognized as surface receptors present in all types of spermatogenic cells. But, there were different degrees of PNA-postive singals emerged on these cells’ membrane. On spermatogonial cell surface, positive staining was strong, in dark brown. Positive signals were similar and weakened on spermatocytes and spermatids. The distribution characteristics of PNA receptors on spermatogenic cells may be related to the development of germ cells as well as the information transmission between germ cells or between germ cells and somatic cells. There were also positive signals on sperm surface and in its acrosome. These PNA receptors may be closely related to the fertilization, such as sperm-oocyte recognition and binding.

Key words: Eriocheir sinensis, spermatogenic cells, peanut agglutinin receptor

CLC Number:

Q954.43

DU Jinmei,LI Lu,MA Zhifang,MU Shumei,KANG Xianjiang. Distribution of peanut agglutinin receptors in spermatogenic cells of Eriocheir sinensis[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(3): 288-292.

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