Species diversity and ecological adaptability of AM fungi in Broussonetia papyrifera from Fengfeng mining area in Hebei Province

doi:10.3969/j.issn.1000-1565.2019.03.009

Abstract

Abstract: In order to explore species diversity of arbuscular mycorrhizal(AM)fungi from Broussonetia papyrifera in Fengfeng mining area of Hebei Province and their ecological adaptability to polluted environment, soil samples from 0-20 cm and 20-40 cm soil layers around Broussonetia papyrifera roots were collected from Yijing Town, Jiecheng Town and Fengfeng Town in Fengfeng Mining Area in October 2016. The AM fungis germplasm resources, ecological distribution and correlation with soil factors were systematically studied. A total of 8 generas and 43 species of AM fungis were identified, Acaulospora and Glomus were of broad-spectrum ecotypes, G.reticulatum, Entrophospora infrequens, G.multiforum were of broad-spectrum ecotypes. Shannon-Weiner index, evenness and species richness of AM fungi in Yijing Town were the highest, while those in Jiecheng Town were the lowest. The content of heavy metal cadmium and zinc exceeded the standard in Fengfeng mining area. The correlation analysis showed that an extremely significant positive correlation was found between the spore density and total glomalin, a significant- DOI:10.3969/j.issn.1000-1565.2019.03.009河北峰峰矿区构树AM真菌物种多样性及生态适应性李华健,贺学礼(河北大学生命科学学院,河北保定 071002)摘要:为探究河北峰峰矿区构树(Broussonetia papyrifera)arbuscular mycorrhizal(AM)真菌物种多样性及其对污染环境的生态适应性,2016年10月从峰峰矿区义井镇、界城镇与峰峰镇样地采集构树根围0~20 cm和20~40 cm土层土壤样品,系统研究AM真菌种质资源、生态分布以及与土壤因子的相关性.共分离鉴定AM真菌8属43种,其中无梗囊霉属(Acaulospora)和球囊霉属(Glomus)为广谱生态型属,网状球囊霉(G.reticulatum)、稀有内养囊霉(Entrophospora infrequens)、凹坑球囊霉(G.multiforum)为广谱生态型种.义井镇AM真菌香农-威纳指数、均匀度和种丰度最高,界城镇最低.峰峰矿区重金属镉和锌含量超标.相关性分析可知,孢子密度与总球囊霉素极显著正相关,与土壤碱解氮显著正相关,与镉含量显著负相关,土壤总球囊霉素与有机碳显著正相关,与镉含量显著负相关.峰峰矿区构树部分地区部分AM真菌孢子具有颜色深、表面网纹间隔致密等生态适应性特征,有利于AM真菌在污染土壤中分布和活动.关键词:AM真菌;构树;重金属污染;土壤因子;河北峰峰矿区中图分类号:X172 文献标志码:A 文章编号:1000-1565(2019)03-0278-10Species diversity and ecological adaptability of AM fungi in Broussonetia papyrifera from Fengfeng mining area in Hebei ProvinceLI Huajian,HE Xueli(College of Life Sciences,Hebei University,Baoding 071002,China)Abstract:In order to explore species diversity of arbuscular mycorrhizal(AM)fungi from Broussonetia papyrifera in Fengfeng mining area of Hebei Province and their ecological adaptability to polluted environment, soil samples from 0-20 cm and 20-40 cm soil layers around Broussonetia papyrifera roots were collected from Yijing Town, Jiecheng Town and Fengfeng Town in Fengfeng Mining Area in October 2016. The AM fungis germplasm resources, ecological distribution and correlation with soil factors were systematically studied. A total of 8 generas and 43 species of AM fungis were identified, Acaulospora and Glomus were of broad-spectrum ecotypes, G.reticulatum, Entrophospora infrequens, G.multiforum were of broad-spectrum ecotypes. Shannon-Weiner index, evenness and species richness of AM fungi in Yijing Town were the highest, while those in Jiecheng Town were the lowest. The content of heavy metal cadmium and zinc exceeded the standard in Fengfeng mining area. The correlation analysis showed that an extremely significant positive correlation was found between the spore density and total glomalin, a significant- 收稿日期:2018-09-23 基金项目:国家自然科学基金资助项目(31270460);河北省自然科学基金资助项目(C2014201060);河北大学研究生创新项目(X201701) 第一作者:李华健(1993—),男,河北邯郸人,河北大学在读硕士研究生,主要从事菌根生物学研究.E-mail:1138345738@qq.com 通信作者:贺学礼(1964—),男,陕西蒲城人,河北大学教授,博士生导师,主要从事生态学研究.E-mail:xuelh1256@aliyun.com第3期李华健等:河北峰峰矿区构树AM真菌物种多样性及生态适应性positive correlation was found between the spore density and soil alkalihydrolyzed nitrogen, a significant positive correlation was found between the spore density and cadmium content, a significant positive correlation was found between the total glomalin and organic carbon, and a significant positive correlation was found between the total glomalin and cadmium content. Some spores of AM fungi from Broussonetia papyrifera in some areas of Fengfeng mining area have the characteristics of dark color, dense surface reticulation spacing and other ecological adaptability, which is conducive to the distribution and activity of AM fungi in contaminated soil.

Key words: arbuscular mycorrhizal fungi, Broussonetia papyrifera, heavy metal pollution, soil factor, Fengfeng mining area of Hebei Province

CLC Number:

X172

LI Huajian,HE Xueli. Species diversity and ecological adaptability of AM fungi in Broussonetia papyrifera from Fengfeng mining area in Hebei Province[J]. Journal of Hebei University (Natural Science Edition), 2019, 39(3): 278-287.

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