CCA analysis of colonization of DSE in the rhizosphere of Ammopiptanthus mongolicus in relation to soil factors

doi:10.3969/j.issn.1000-1565.2017.05.009

Abstract

Abstract: Ammopiptanthus mongolicus is the only evergreen broadleaf shrub grown in the northwest China that has the characteristics of cold and drought resistance, salt resistance and wind resistance. It plays an important role in windbreak and soil and water conservation. To elucidate the spatial distribution of dark septate endophytes(DSE)and the relationship of DSE with soil factors associated with the desert plant A. mongolicus based on canonical correspondence analysis(CCA), the author sampled from five different sites in northwest China in July 2015, which are Wuhai, Dengkou, Alxa, Minqin and Shapotou, respectively. Root samples were collected from four robust A. mongolicus,and soil samples were taken from the rhizosphere of A. mongolicus in five sites and divided into five depth intervals, 0-10 cm, 10-20 cm,20-30 cm, 30-40 cm and 40-50 cm, respectively. The results showed that the roots of A. mongolicus could be infected highly by DSE and formed a typical type of septate hyphae and microsclerotia. The distribution of DSE was significantly affected by ecological conditions. There were significant differences in distribution of DSE among the five sites. The colonization rates of DSE among the five sites exhibited a pattern as Alxa>Wuhai>Shapotou>Minqin>Dengkou. The average colonization rate of five sites was 52.02%, which showed that A.mongolicus and DSE could form a symbiotic relationship. There were also significant differences in soil factors among different sites. In the same site, DSE distribution and soil indicators had the maximum values in the profile of a surface layer and showed the trend of decreasing with the depth of the profile. The results of CCA showed that DSE hyphae, microsclerotia, colonization rate and colonization intensity in the axis of CCA got better differentiation. The species-environment biplots were drawn based on the result of CCA. The relationships between the colonization of DSE and the seven soil factors were clearly revealed on these biplots. Accoding to the biplots, the main soil factors of five sites were different. The acid phosphatase(ACP), alkaline phosphatase(ALP)and available phosphate were found to be main soil factors affecting the hypha colonization of DSE in Wuhai. The main soil factors of Dengkou were available nitrogen and organic carbon. The hypha colonization of DSE in Alxa were mainly affected by ALP and pH. The main soil factors affecting the hypha colonization of DSE were pH, ALP and Urease, in Minqin. And the main soil factors affecting the hypha colonization of DSE in Shapotou were ACP, Urease and available nitrogen. The research results showed that the distribution of DSE in different regions was affected by different soil factors, which could provide the basis for the evaluation and improvement of soil conditions in the desert area.

Key words: dark septate endophytes(DSE), colonization rate, soil factors, canonical correspondence analysis

CLC Number:

Q939.96

WANG Shaojie, HOU Lifeng, XIE Linlin, HU Congcong, HE Xueli. CCA analysis of colonization of DSE in the rhizosphere of Ammopiptanthus mongolicus in relation to soil factors[J]. Journal of Hebei University (Natural Science Edition), 2017, 37(5): 497-507.

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