Colonization of dark septate endophytes in roots of different plants and its correlation with soil factors in extreme arid desert environment

doi:10.3969/j.issn.1000-1565.2018.01.012

Abstract

Abstract: In order to elucidate the colonization patterns of dark septate endophytes(DSE)in roots of different plants and its correlation with soil factors in extreme arid desert environment, the samples of soil and roots of Ephedra przewaii, Reaumuria soongorica, Sympegma regelii, Nitraria sphaerocarpa and Salsola passerina were collected in Anxi, Gansu Province, July 2016.The results showed that DSE could infect the roots of five desert plants, and formed typical melanized septate hyphae and microsclerotia; Soil acid phosphatase, urease, total extractable glomalin and soil humidity significantly affected the DSE colonization intensity; The plant species had a significantly direct effect on the total and hyphal colonization of DSE, and indirectly affected the DSE colonization by the soil temperature and humidity, urease, acid phosphatase. The results supported the conclusion that plant species and soil factors were important factors affecting DSE colonization.- DOI:10.3969/j.issn.1000-1565.2018.01.012极旱荒漠植物深色有隔内生真菌定殖及其与土壤因子的相关性葛佳丽,张琳琳,李欣玫,强薇,贺学礼(河北大学生命科学学院,河北保定 071002)摘要:为了探明荒漠环境深色有隔内生真菌(dark septate endophytes, DSE)生态分布和定殖状况,于2016年7月从甘肃安西极旱荒漠国家自然保护区采集膜果麻黄(Ephedra przewaii)、红砂(Reaumuria soongorica)、合头草(Sympegma regelii)、泡泡刺(Nitraria sphaerocarpa)和珍珠猪毛菜(Salsola passerina)等5种植物根际和根围0~30 cm土层土壤样品和根样,研究了不同荒漠植物DSE定殖及其与土壤因子的相关性. 结果发现:5种荒漠植物根系均能被DSE侵染,DSE分布和定殖具有明显的空间异质性,并与土壤因子密切相关;不同根系来源样品DSE定殖率与土壤湿度、脲酶存在极显著负相关性;土壤湿度、酸性磷酸酶和脲酶显著影响DSE定殖强度;植物种类对DSE菌丝定殖率和总定殖率具有显著直接影响,并通过土壤温湿度、脲酶、酸性磷酸酶间接影响DSE定殖.可见,植物种类和土壤因子是影响DSE定殖的重要因素.关键词:深色有隔内生真菌;生态分布;植物种类;极旱荒漠环境中图分类号:Q934 文献标志码:A 文章编号:1000-1565(2018)01-0073-10Colonization of dark septate endophytes in roots of different plantsand its correlation with soil factors in extreme arid desert environmentGE Jiali,ZHANG Linlin,LI Xinmei,QIANG Wei,HE Xueli(College of Life Sciences,Hebei University, Baoding 071002,China)Abstract: In order to elucidate the colonization patterns of dark septate endophytes(DSE)in roots of different plants and its correlation with soil factors in extreme arid desert environment, the samples of soil and roots of Ephedra przewaii, Reaumuria soongorica, Sympegma regelii, Nitraria sphaerocarpa and Salsola passerina were collected in Anxi, Gansu Province, July 2016.The results showed that DSE could infect the roots of five desert plants, and formed typical melanized septate hyphae and microsclerotia; Soil acid phosphatase, urease, total extractable glomalin and soil humidity significantly affected the DSE colonization intensity; The plant species had a significantly direct effect on the total and hyphal colonization of DSE, and indirectly affected the DSE colonization by the soil temperature and humidity, urease, acid phosphatase. The results supported the conclusion that plant species and soil factors were important factors affecting DSE colonization.- 收稿日期:2017-03-21 基金项目:国家自然科学基金资助项目(31470533) 第一作者:葛佳丽(1991—),女,山西临汾人,河北大学在读硕士研究生.E-mail:1728099876@qq.com 通信作者:贺学礼(1963—),男,陕西蒲城人,河北大学教授,博士生导师,主要从事植物生态学和土壤生态学研究.E-mail: xuelh1256@aliyun.com第1期葛佳丽等:极旱荒漠植物深色有隔内生真菌定殖及其与土壤因子的相关性

Key words: dark septate endophytes(DSE), ecological distribution, plant species, extreme arid desert environment

CLC Number:

Q934

GE Jiali,ZHANG Linlin,LI Xinmei,QIANG Wei,HE Xueli. Colonization of dark septate endophytes in roots of different plants and its correlation with soil factors in extreme arid desert environment[J]. Journal of Hebei University (Natural Science Edition), 2018, 38(1): 73-82.

References

[1] 甘肃安西极旱荒漠国家级自然保护区管理局.甘肃安西极旱荒漠国家级自然保护区三期综合科考报告[M].兰州:甘肃人民出版社,2014.
[2] 刘茂军,张兴涛,赵之伟.深色有隔内生真菌(DSE)研究进展[J].菌物学报,2009,28(6):889-894. LIU M J, ZHANG X T, ZHAO Z W. Advances in the research of dark septate endophytes[J].Mycosystema, 2009,28(6):889-894.
[3] MANDYAM K,JUMPPONEN A. Seeking the elusive function of the root-colonising dark septate endophytic fungi[J]. Studies in Mycology, 2005, 53: 173-189.DOI: 10.3114/sim.53.1.173.
[4] JUMPPONEN A, MATTSON K G, TRAPPE J M. Mycorrhizal functioning of Phialocephala fortinii with Pinus contorta on glacier forefront soil: interactions with soil nitrogen and organic matter[J]. Mycorrhiza, 1998, 7(5): 261-265.DOI: 10.1007/s005720050190.
[5] BARROW J R, AALTONEN R E. Evaluation of the internal colonization of Atriplex canescens(Pursh)Nutt. roots by dark septate fungi and the influence of host physiological activity[J]. Mycorrhiza, 2001, 11(4): 199-205.DOI:10.1038/sj.embor.7400790.
[6] 孙茜, 贺超, 贺学礼, 等. 沙冬青与伴生植物深色有隔内生真菌定殖规律及其与土壤因子的相关性[J]. 植物生态学报, 2015, 39(9): 878-889.DOI: 10.17521/cjpe.2015.0084. SUN Q, HE C, HE X L, et al. Colonization of dark septate endophytes in roots of Ammopiptanthus mongolicus and its associated plants as influenced by soil properties[J]. Chinese Journal of Plant Ecology, 2015, 39(9):878-889.DOI: 10.17521/cjpe.2015.0084.
[7] XIE L L, HE X L, WANG K, et al. Spatial dynamics of dark septate endophytes in the roots and rhizospheres of Hedysarum scoparium in northwest China and the influence of edaphic variables[J]. Fungal Ecology, 2017, 26: 135-143.DOI:10.1016/j.funeco.2017.01.007.
[8] MASSENSSINI A M, BONDUKI V H A, TOTOLA M R, et al. Arbuscular mycorrhizal associations and occurrence of dark septate endophytes in the roots of Brazilian weed plants[J]. Mycorrhiza, 2014, 24(2): 153-159.DOI:10.1007/s00572-013-0519-6.
[9] BONFIM J A, VASCONCELLOS R L F, BALDESIN L F, et al. Dark septate endophytic fungi of native plants along an altitudinal gradient in the Brazilian Atlantic forest[J]. Fungal Ecology, 2016, 20: 202-210.DOI:10.1016/j.funeco.2016.01.008.
[10] SAMAGA P V, RAI V R. Diversity and bioactive potential of endophytic fungi from Nothapodytes foetida, Hypericum mysorense and Hypericum japonicum collected from Western Ghats of India[J]. Annals of microbiology, 2016, 66(1): 229-244.DOI:10.1007/s13213-015-1099-9.
[11] KAUPPINEN M, RAVEALA K, WALI P R, et al. Contrasting preferences of arbuscular mycorrhizal and dark septate fungi colonizing boreal and subarctic Avenella flexuosa[J].Mycorrhiza, 2014, 24(3): 171-177.DOI:10.1007/s00572-013-0526-7.
[12] KNAPP D G, KOVACS G M, ZAJTA E, et al. Dark septate endophytic pleosporalean genera from semiarid areas[J]. Persoonia Molecular Phylogeny and Evolution of Fungi, 2015, 35: 87-100.DOI:10.3767/003158515X687669.
[13] LUGO M A, REINHART K O, MENOYO E, et al. Plant functional traits and phylogenetic relatedness explain variation in associations with root fungal endophytes in an extreme arid environment[J]. Mycorrhiza, 2015, 25(2): 85-95.DOI:10.1007/s00572-014-0592-5.
[14] WU L Q, LV Y L, MENG Z X, et al. The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir[J]. Mycorrhiza, 2010, 20(2): 127-135.DOI:10.1007/s00572-009-0268-8.
[15] KHASTINI R O, OHTA H, NARISAWA K. The role of a dark septate endophytic fungus, Veronaeopsis simplex Y34, in fusarium disease suppression in Chinese cabbage[J]. Journal of Microbiology, 2012, 50(4): 618-624.DOI:10.1007/s12275-012-2105-6.
[16] LIKAR M, REGVAR M. Isolates of dark septate endophytes reduce metal uptake and improve physiology of Salix caprea L[J]. Plant and Soil, 2013, 370(1-2): 593-604.DOI:10.1007/s11104-013-1656-6.
[17] MANDYAM K, JUMPPONEN A. Unraveling the dark septate endophyte functions: insights from the Arabidopsis model[M].India: Springer India, 2014: 115-141.DOI:10.1007/978-81-322-1575-2_6.
[18] 宋勇春, 李晓林, 冯固. 菌根真菌磷酸酶活性对红三叶草生境中土壤有机磷亏缺的影响[J]. 生态学报, 2001, 21(7): 1130-1135. SONG Y C, LI X L, FENG G. Effect of phosphatase activity on soil organic phosphorus loss in the environment of clover growth[J]. Acta Ecologica Sinica, 2001, 21(7):1130-1135.
[19] HOFFMANN G G, TEICHER K. A colorimetric technique for determining urease activity in soil[J]. Dung Boden, 1961, 95: 55-63.
[20] WRIGHT S F, UPADHYAYA A. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi[J]. Plant and Soil, 1998, 198(1): 97-107.
[21] 贺学礼, 许珂, 郭辉娟. 黄芩根围AM真菌分布与土壤碳氮相关性研究[J]. 河北农业大学学报, 2011, 34(5):63-68. HE X L, XU K, GUO H J. Relationship between distribution of AM fungi in the rhizosphere of Scutellaria baicalensis and soil C and N[J]. Journal of Hebei Agricultural University, 2011, 34(5): 63-68.
[22] 贺学礼,王雷,牛凯,等. 菊花AM真菌与球囊霉素季节性分布[J]. 西北农业学报,2013,22(01):162-167. HE X L, WANG L, NIU K, et al. Seasonal distribution of AM fungi and glomalin in the rhizosphere of Dendranthema morifolium[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2013,22(01):162-167.
[23] PHILLIPS J M, HAYMAN D S. Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection[J]. Transactions of the British mycological Society, 1970, 55(1): 158-161.
[24] BIERMANN B, LINDERMAN R G. Quantifying vesicular-arbuscular mycorrhizae: a proposed method towards standardization[J]. New Phytologist, 1981, 87(1): 63-67.DOI: 10.1111/j.1469-8137.1981.tb01690.x
[25] PETERSON R L, WAGG C, PAUTLER M. Associations between microfungal endophytes and roots: do structural features indicate function[J]. Botany, 2008, 86(5): 445-456.DOI: 10.1139/B08-016.
[26] MAYERHOFER M S, KHANERNAG G, HARPER K A. The effects of fungal root endophytes on plant growth: a meta-analysis[J]. Mycorrhiza, 2013, 23(2): 119-128.DOI:10.1007/s00572-012-0456-9.
[27] 王孝安.安西荒漠植物群落和优势种的分布与环境的关系[J].植物学报,1998,40(11):70-75.
[28] THANGAVELU M, RAJI M. Arbuscular mycorrhizal and dark septate endophyte fungal associations in Asparagus[J]. Turkish Journal of Botany, 2016, 40(6): 662-675.DOI:10.3906/bot-1602-11.
[29] MANDYAM K, FOX C, JUMPPONEN A. Septate endophyte colonization and host responses of grasses and forbs native to a tallgrass prairie[J]. Mycorrhiza, 2012, 22(2): 109-119.DOI:10.1007/s00572-011-0386-y.
[30] DELLA MONICA I F, SAPARRAT M C N, GODEAS A M, et al. The co-existence between DSE and AMF symbionts affects plant P pools through P mineralization and solubilization processes[J]. Fungal Ecology, 2015, 17: 10-17.DOI:10.1016/j.funeco.2015.04.004.