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25 January 2018, Volume 38 Issue 1

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Homogeneous Rota-Baxter 3-Lie Algebras(Ⅰ)

BAI Ruipu,KANG Chuangchuang,MA Yue,HOU Shuai,BA Yi

2018, 38(1):  1-6.  DOI: 10.3969/j.issn.1000-1565.2018.01.001

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Homogeneous Rota-Baxter operators R on the infinite dimensional simple 3-Lie Algebra A_ω=∑_m∈ZFL_m are Rota-Baxter operators which satisfy R(L_m)=f(m)L_m, where f:Z→Fis a function. Since Rota-Baxter operators of weight λ with λ≠0 on 3-Lie algebras are completely determined by the case λ=1, the homogeneous Rota-Baxter operators of weight 1 on A_ω with |W₁|<∞ are discussed. Five 3-Lie algebras(A,［,,］_j)are constructed by the simple 3-Lie algebra A_ω and its homogeneous Rota-Baxter operators.And it is proved that 3-Lie algebras(A,［,,］_j)are all homogeneous Rota-Baxter 3-Lie algebras.

Weierstrass’s theorem in L_p spaces of random continuous function

CHEN Yingwei,WANG Zhijun

2018, 38(1):  7-11.  DOI: 10.3969/j.issn.1000-1565.2018.01.002

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The purpose of this article is to investigate polynomial approximation properties of random continuous function by the probability method. We obtained the qualitative estimation by random Bernstein’s operator with L_p norm and extended Weierstrass's theorem to random continuous function spaces further.

Research progress of n-type oxides thermoelectric materials

WANG Shufang, GAO Linjie

2018, 38(1):  12-21.  DOI: 10.3969/j.issn.1000-1565.2018.01.003

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Oxide thermoelectric materials have high thermal and chemical stability in air, which show promising potential in the field of high temperature thermoelectric applications.Compared with p-type oxide thermoelectric materials, the ZT values of n-type oxides are generally low, limiting the development of oxides-based thermoelectric devices.This paper discussed in detail the research progresses of the present representative n-type oxide thermoelectric materials, analyzed the preparation methods, the physical mechanisms, and the effects of electrical and thermal modulation on the thermoelectric performances of these materials.Some new ideas for further improvment of the performances of n-type oxide thermoelectrical materials were proposed.

Factors influencing the formation and fracture of Taylor cone

LI Qing,TAN Guozheng,JI Xiaoxiao, YIN Yuchao

2018, 38(1):  22-27.  DOI: 10.3969/j.issn.1000-1565.2018.01.004

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In order to study the microscopic movement process of Taylor cone in the process of electrostatic atomization, the formation and fracture of Taylor cone in different liquid electrostatic atomization process were studied by using the cavity electrostatic atomizer.The Taylor cone taper angle,the relationship between the length and the minor axis, the cone formation and fracture cycle and other related parameters were analyzed.The results show that the formation and fracture of Taylor cone are divided into two stages: energy storage and fracture.The discharge field strength, liquid viscosity and surface tension are the main factors affecting the Taylor cone formation and fracture.The viscosity and surface tension of liquid, the charge distribution in discharge space, the distribution of discharge field strength and the surface charge distribution of Taylor cone determine the final shape and period of motion of the Taylor cone.

Application of 7-hydroxylcoumarin-aldehyde in the detection of hypochlorite

YIN Caixia, XIONG Kangming, HUO Fangjun

2018, 38(1):  28-32.  DOI: 10.3969/j.issn.1000-1565.2018.01.005

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7-hydroxylcoumarin-aldehyde can be used to detect hypochlorite in PBS(pH=7.4)solution.The results of spectra show that the probe has a fluorescence enhancement property for hypochlorite recognition in PBS(pH=7.4)solution, and the color of the solution changes from light yellow to deep-yellow, and the fluorescence of the solution changes from weak fluorescence to deep-blue fluorescence.The recognition response of the probe to hypochlorite can be rapidly completed within 1s, and the detection limit is found to be as low as 51.75 nmol/L.In addition, the probe can be used for fluorescence imaging of hypochlorite in HepG2 cells.

Determination of plasticizer in bottled water by low density solvent based vortex assisted dispersive liquid-liquid microextraction combined with gas chromatography

ZHANG Qi, MA Yaojia, AI Lianfeng, L

2018, 38(1):  33-40.  DOI: 10.3969/j.issn.1000-1565.2018.01.006

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A novel method of low-density solvent-based vortex-assisted dispersive liquid-liquid microextraction(DLLME)was developed for determination of plasticizer in bottled water by gas chromatography-flame ionization detection(GC-FID).Conventional DLLME methods usually employ organic solvents heavier than water as the extraction solvents.On the contrary, in this proposed extraction procedure, a mixture of low-density extraction solvent(toluene and n-hexane with V/V=1∶1)and dispersive solvent(acetonitrile)was injected into the aqueous sample solution to form an emulsion with vortex dispersed mode.The upper layer was collected and analyzed by GC after centrifugation.The enrichment factor was as high as 171.Under the optimized conditions, the proposed method provided a good linearity(R²=0.998 2~0.999 2)and the- DOI:10.3969/j.issn.1000-1565.2018.01.006基于轻质萃取剂的涡旋辅助分散液液微萃取-气相色谱法测定瓶装水中塑化剂张琪¹,马瑶佳¹,艾连峰²,吕运开¹(1.河北大学 化学与环境科学学院,河北 保定 071002;2.河北出入境检验检疫局,河北 石家庄 050051)摘 要:以密度比水小的溶剂为萃取剂,建立了涡旋辅助分散液液微萃取-气相色谱检测瓶装水中塑化剂的方法.传统分散液液微萃取技术一般采用密度大于水的有机溶剂为萃取剂.本方法以密度比水小的轻质溶剂正己烷-甲苯(1∶1,体积比)为萃取剂,乙腈为分散剂,涡旋辅助分散,离心后获得富集相,进行气相色谱检测,富集倍数高达171倍,具有良好的线性关系(相关系数为0.998 2~0.999 2)和精密度(3.1%~11.8%),以及较低的方法检出限(1.43~12.05 μg/L),加标回收率为81.2%~107.8%.该方法简便,快速,环保,可以用于测定实际水样中塑化剂.关键词:气相色谱;涡旋辅助;分散液液微萃取;塑化剂;瓶装水中图分类号:O657.7 文献标志码:A 文章编号:1000-1565(2018)01-0033-08Determination of plasticizer in bottled water by low density solventbased vortex assisted dispersive liquid-liquid microextractioncombined with gas chromatographyZHANG Qi¹, MA Yaojia¹, AI Lianfeng², Lü Yunkai¹(1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China;2.Hebei Entry-Exit Inspection and Quarantine Bureau,Shijiazhuang 050051, China)Abstract: A novel method of low-density solvent-based vortex-assisted dispersive liquid-liquid microextraction(DLLME)was developed for determination of plasticizer in bottled water by gas chromatography-flame ionization detection(GC-FID).Conventional DLLME methods usually employ organic solvents heavier than water as the extraction solvents.On the contrary, in this proposed extraction procedure, a mixture of low-density extraction solvent(toluene and n-hexane with V/V=1∶1)and dispersive solvent(acetonitrile)was injected into the aqueous sample solution to form an emulsion with vortex dispersed mode.The upper layer was collected and analyzed by GC after centrifugation.The enrichment factor was as high as 171.Under the optimized conditions, the proposed method provided a good linearity(R²=0.998 2~0.999 2)and the- 收稿日期:2017-08-20 基金项目:国家自然科学基金资助项目(21375032);河北省自然科学基金资助项目(B2016201213) 第一作者:张琪(1992—),女,河北石家庄人,河北大学在读硕士研究生,从事食品安全分析研究.E-mail:zqsys1017@163.com 通信作者:吕运开(1967—),男,河北保定人,河北大学教授,博士,主要从事色谱分析与食品安全分析研究.E-mail:lvyunkai@hbu.edu.cn第1期张琪等:基于轻质萃取剂的涡旋辅助分散液液微萃取-气相色谱法测定瓶装水中塑化剂relative standard deviations(RSDs)were in the range of 3.1%~11.8%.The limits of detection(S/N=3)were in the range of 1.43~12.05 μg/L and the recoveries at three spiked levels were in the range of 81.2%~107.8%.It is a convenient, speedy, and environmentally benign for the routine analysis of plasticizer in water samples.

Effects of different proportions of 18α,β-glycyrrhizic acids on lipid metabolism on alcoholic liver injury

YANG Sa, SUN Xiaoke, MENG Xiangbo, ZHOU Chengyan, ZHAO Yanyan

2018, 38(1):  41-48.  DOI: 10.3969/j.issn.1000-1565.2018.01.007

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To investigate the effect of different proportions of 18α,β-glycyrrhizic acids on lipid metabolism on alcoholic liver injury and screen out their optimal combination proportion, the rats were randomly divided into ten groups: normal control group, model control group, siibin group and glycyrrhizic treatment group 1-7(α∶β=10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10).After 4 weeks, the rats were sacrificed.The serum and hepatic TC, TG, HDL and LDL levels and fecal TC and TG levels were measured.And liver was stained with oil red O.We investigated the effect of different proportions of 18α,β-glycyrrhizic acid on lipid metabolism on alcoholic liver injury and further screen out optimal combination proportion.The TC, TG, HDL and LDL levels were significant different among different glycyrrhizic treatment group, and the glycyrrhizic treatment group 5 is the optimal combination proportion.Our results show that glycyrrhizic treatment could modulate lipid metabolism on alcoholic liver injury, and the- DOI:10.3969/j.issn.1000-1565.2018.01.007甘草酸差向异构体不同配比对酒精性肝损伤脂质代谢的影响杨飒,孙晓可,孟祥波,周程艳,赵燕燕(河北大学 药学院,河北省药物质量分析控制重点实验室,河北 保定 071002)摘 要:研究18α-甘草酸和18β-甘草酸不同配比对酒精性肝损伤脂质代谢的影响,筛选最佳配比,为临床合理用药提供依据.大鼠随机分为正常组、酒精性肝损伤组、水飞蓟宾阳性组、甘草酸给药1~7组(18α-甘草酸和18β-甘草酸体积比依次为10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10),造模4周后,麻醉处死.检测脂质代谢指标血清和肝脏中的甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)和粪便中的TC,TG;氧化应激指标超氧化物歧化酶(SOD),谷胱甘肽(GSH),丙二醛(MDA);进行HE染色以及油红O染色.与模型组相比,给药组的TC,TG,HDL及LDL水平均有显著性变化,其中给药5组的效果最佳.甘草酸对于酒精性肝损伤的脂质代谢有较好的治疗作用,18α-甘草酸和18β-甘草酸最佳体积配比为4∶6.关键词:18α-甘草酸;18β-甘草酸;不同配比;酒精性肝损伤;脂质代谢中图分类号:R285.5 文献标志码:A 文章编号:1000-1565(2018)01-0041-08Effects of different proportions of 18α,β-glycyrrhizic acids on lipidmetabolism on alcoholic liver injuryYANG Sa, SUN Xiaoke, MENG Xiangbo, ZHOU Chengyan, ZHAO Yanyan(Key Laboratory of Pharmaceutical Quality Control of Hebei ProvinceCollege of Pharmaceutical Sciences, Hebei University, Baoding 071002, China)Abstract: To investigate the effect of different proportions of 18α,β-glycyrrhizic acids on lipid metabolism on alcoholic liver injury and screen out their optimal combination proportion, the rats were randomly divided into ten groups: normal control group, model control group, siibin group and glycyrrhizic treatment group 1-7(α∶β=10∶0,8∶2,6∶4,5∶5,4∶6,2∶8,0∶10).After 4 weeks, the rats were sacrificed.The serum and hepatic TC, TG, HDL and LDL levels and fecal TC and TG levels were measured.And liver was stained with oil red O.We investigated the effect of different proportions of 18α,β-glycyrrhizic acid on lipid metabolism on alcoholic liver injury and further screen out optimal combination proportion.The TC, TG, HDL and LDL levels were significant different among different glycyrrhizic treatment group, and the glycyrrhizic treatment group 5 is the optimal combination proportion.Our results show that glycyrrhizic treatment could modulate lipid metabolism on alcoholic liver injury, and the- 收稿日期:2017-08-15 基金项目:河北省自然科学基金资助项目(H2013201203);河北大学医学学科专项资金建设项目(2014A1003) 第一作者:杨飒(1992—),女,河北保定人,河北大学在读研究生.E-mail:yangsa721@163.com 通信作者:赵燕燕(1960—),女,天津人,河北大学教授,主要从事药学以及将现代分离技术用于临床、药学、食品、环境等方面的研究工作.E-mail:zhaoyany606@163.com第1期杨飒等:甘草酸差向异构体不同配比对酒精性肝损伤脂质代谢的影响optimal combination proportion is 4∶6.

Simultaneous determination of metformin hydrochloride and glimepiride in compound metformin tablets by CZE

LI Haiying,DUAN Yumeng, JIA Bei, ZUO Yueyue, YANG Wenzhi

2018, 38(1):  49-54.  DOI: 10.3969/j.issn.1000-1565.2018.01.008

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To develop a capillary zone electrophoresis(CZE)method for the simultaneous quantitative analysis of metformin hydrochloride(MET)and glimepiride(GLM)in compound metformin tablets.CZE with UV detector at 229 nm was applied using a silica capillary column(50 μm×80 cm, effective length of 50 cm).The sample was employed with an electrokinetic injection of 5 kV for 12 s.The separation buffer was composed of 5 mmol/L sodium tetraborate buffer(pH 7.0).CE method was performed at room temperature and the running voltage was 17 kV.The results showd that the linear range of MET and GLM were 20~1 800 μg/mL(R=0.972 7)and 5~200 μg/mL(R = 0.990 6), respectively.The average recoveries of GLM and MTE at high, middle and low concentrations were 101.0%, 103.2%, 101.3% and 100.5%,100.7%, 99.8%, respectively.The contents of MET and GLM in three samples were 109.6%,- DOI:10.3969/j.issn.1000-1565.2018.01.008CZE法同时测定复方二甲双胍片中盐酸二甲双胍和格列美脲含量李海鹰,段雨萌,贾蓓,左月月,杨文智(河北大学 药学院,河北省药物质量分析控制重点实验室,河北 保定 071002)摘 要:采用毛细管区带电泳法(CZE)同时测定复方二甲双胍片中格列美脲(Glimepiride,GLM)和盐酸二甲双胍(Metformin hydrochloride,MET)含量.选用未涂层石英毛细管柱(50 μm×80 cm,有效长度50 cm),以5 mmol/L硼砂缓冲液(pH=7)为分离缓冲溶液,运行电压17 kV,电进样5 kV×12 s,紫外检测器(λ=229 nm).结果表明:MET和GLM质量浓度分别在20~1 800 μg/mL(R=0.972 7)和5~200 μg/mL(R=0.990 6)内与峰面积呈良好的线性关系,MET和GLM 2种药物的高、中、低3个质量浓度平均回收率分别为101.0%、103.2%、101.3%和100.5%、100.7%、99.8%.自制的3批复方降糖片中MET标示量质量分数分别为109.6%、108.2%、108.5%,GLM的标示量质量分数分别为100.9%、100.3%、104.5%.CZE法可用于复方二甲双胍片中MET和GLM的含量测定,方法简便,准确性好.关键词:盐酸二甲双胍;格列美脲;毛细管区带电泳;含量测定中图分类号:O657.7 文献标志码:A 文章编号:1000-1565(2018)01-0049-06Simultaneous determination of metformin hydrochloride andglimepiride in compound metformin tablets by CZE LI Haiying,DUAN Yumeng, JIA Bei, ZUO Yueyue, YANG Wenzhi(Key Laboratory of Pharmaceutical Quality Control of Hebei Province,College of Pharmacy,Hebei University, Baoding 071002, China)Abstract: To develop a capillary zone electrophoresis(CZE)method for the simultaneous quantitative analysis of metformin hydrochloride(MET)and glimepiride(GLM)in compound metformin tablets.CZE with UV detector at 229 nm was applied using a silica capillary column(50 μm×80 cm, effective length of 50 cm).The sample was employed with an electrokinetic injection of 5 kV for 12 s.The separation buffer was composed of 5 mmol/L sodium tetraborate buffer(pH 7.0).CE method was performed at room temperature and the running voltage was 17 kV.The results showd that the linear range of MET and GLM were 20~1 800 μg/mL(R=0.972 7)and 5~200 μg/mL(R = 0.990 6), respectively.The average recoveries of GLM and MTE at high, middle and low concentrations were 101.0%, 103.2%, 101.3% and 100.5%,100.7%, 99.8%, respectively.The contents of MET and GLM in three samples were 109.6%,- 收稿日期:2017-04-09 基金项目:河北省自然科学基金面上项目(H2018201045,H2017201052);河北省高等学校科学技术研究项目(DZ2016102);河北大学大学生创新创业计划训练项目(201710075014,201710075074) 第一作者:李海鹰(1973—),女,内蒙古集宁人,河北大学副教授,博士,主要从事药物分析及药物制剂方面的研究.E-mail: lihylihy@163.com第1期李海鹰等:CZE法同时测定复方二甲双胍片中盐酸二甲双胍和格列美脲含量 108.2%,108.5%, and 100.9%, 100.3%, 104.5%, respectively.The CZE method is rapid, simple, convenient and accurate.It can be used to detect the content of MET and GLM in compound metformin tablet.

A new recorded genus and species of corinnid in China (Araneae: Corinnidae)

ZHANG Feng, JIN Chi

2018, 38(1):  55-58.  DOI: 10.3969/j.issn.1000-1565.2018.01.009

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The genus Pranburia Deeleman-Reinhold, 1993, described originally from Thailand and also found in Cambodia, Laos and Malaysia, is reported for the first time from China.The type species P.mahannopi is redescribed and illustrated here.The specimens checked in this paper are deposited in the Museum of Hebei University(MHBU), Baoding, China.All measurements are in millimeters.

Expression of Chinese shrimp Fenneropenaeus chinensis ALFFc in Pichia pastoris

TANG Ting, LU Meike, MA Xinjian, LI Ting, LIU Fengsong

2018, 38(1):  59-64.  DOI: 10.3969/j.issn.1000-1565.2018.01.010

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Anti-lipopolysaccharide factor(ALF)is a low molecular mass protein with antimicrobial activities and plays an important role in immune response against microbial invasion.Thus, they are considered to be good substitutes for traditional antibiotics.In the present study, the prokaryotic recombinant expression vector “pET-DsbA-ALFFc” was used as the template of PCR, which was constructed in previous study.EcoRI and NotI restriction sites were added to 5’ and 3’ ends of the ALFFc.The ALFFc fragment was ligated to pPIC9K vector to construct a recombinant expression vector pPIC9K-ALFFc, which then was transformed into competent Pichia pastoris GS115.Recombinant ALFFc(rALFFc)was induced with methanol and purified by Ni-chelating affinity chromatography.SDS-PAGE and Western blot analysis indicated that the rALFFc was expressed successfully in P.pastoris.

Effect of aberrant O-glycosylation of GRP78 on invasive and metastatic ability of breast cancer cell line

LIU Yang, XIE Rong, WANG Xiumin, ZHAO Lina, WANG Xi, WU Chen

2018, 38(1):  65-72.  DOI: 10.3969/j.issn.1000-1565.2018.01.011

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To investigate the effect of aberrant O-glycosylation of GRP78 on invasive and metastatic ability of breast cancer cell line MCF-7, Human GRP78 cDNA was cloned and O-linked glycosylation site of GRP78 was mutated by site-directed mutagenesis.The expressions of exogenous wild-type and mutant GRP78 in MCF-7 were confirmed by Western blot.Wound repair assays and transwell assays were performed to study the effect of GRP78 mutants on its biological functions.Wound repair assays showed that the ability of cell migration in MCF-7 cells transfected with mutant GRP78 was significantly decreased compared to that of the cells transfected with wild-type GRP78.Transwell assay showed that the invading cell number of mutant group was less than that of cells transfected with wild-type GRP78,which suggested that the deficiency in O-glycosylation of GRP-78 abolished its ability of cell invasion and migration.These findings laid a good foundation for further study on the effect of O-glycosylation on the biological functions of GRP78.- DOI:10.3969/j.issn.1000-1565.2018.01.011GRP78异常O-糖基化对乳腺癌细胞侵袭迁移的影响刘洋,谢蓉,王秀敏,赵丽娜,王茜,吴琛(河北大学 生命科学学院,河北 保定 071002)摘 要:为了探索O-糖基化异常对GRP78促进人乳腺癌MCF-7细胞侵袭转移能力的影响,克隆人GRP78基因,利用点突变技术,构建了GRP78野生型及T648A、T648G 2个糖基化位点突变真核表达载体.Western blot检测外源性GRP78在MCF-7细胞中的表达.利用划痕修复实验和transwell侵袭小室实验,研究GRP78第648位Thr突变后引起的异常糖基化对其生物学功能的影响.划痕实验结果显示与野生型相比,转染突变体的MCF-7细胞的覆盖面积较少.Transwell检测结果显示突变体转染组的侵袭细胞数少于对照组,说明GRP78 O-糖基化缺失后明显降低了其促进MCF-7细胞侵袭迁移的能力.本研究为深入探讨O-糖基化在介导GRP78生物学功能中的作用机制奠定了基础.关键词:葡萄糖调节蛋白78(GRP78);异常O-糖基化;定点突变中图分类号:Q78 文献标志码:A 文章编号:1000-1565(2018)01-0065-08Effect of aberrant O-glycosylation of GRP78 on invasiveand metastatic ability of breast cancer cell line LIU Yang, XIE Rong, WANG Xiumin, ZHAO Lina, WANG Xi, WU Chen(College of Life Sciences, Hebei University, Baoding 071002, China)Abstract: To investigate the effect of aberrant O-glycosylation of GRP78 on invasive and metastatic ability of breast cancer cell line MCF-7, Human GRP78 cDNA was cloned and O-linked glycosylation site of GRP78 was mutated by site-directed mutagenesis.The expressions of exogenous wild-type and mutant GRP78 in MCF-7 were confirmed by Western blot.Wound repair assays and transwell assays were performed to study the effect of GRP78 mutants on its biological functions.Wound repair assays showed that the ability of cell migration in MCF-7 cells transfected with mutant GRP78 was significantly decreased compared to that of the cells transfected with wild-type GRP78.Transwell assay showed that the invading cell number of mutant group was less than that of cells transfected with wild-type GRP78,which suggested that the deficiency in O-glycosylation of GRP-78 abolished its ability of cell invasion and migration.These findings laid a good foundation for further study on the effect of O-glycosylation on the biological functions of GRP78.- 收稿日期:2017-08-02 基金项目:国家自然科学基金资助项目(31670812);河北省高层次人才资助项目(CY201602);河北大学研究生创新资助项目(X201724) 第一作者:刘洋(1988—),男,河北秦皇岛人,河北大学在读硕士研究生.E-mail:dra04@sina.com 通信作者:吴琛(1977—),女,河北保定人,河北大学教授,博士,主要从事肿瘤分子生物学研究.E-mail:dawnwuchen@l63.com第1期刘洋等:GRP78异常O-糖基化对乳腺癌细胞侵袭迁移的影响

Colonization of dark septate endophytes in roots of different plants and its correlation with soil factors in extreme arid desert environment

GE Jiali,ZHANG Linlin,LI Xinmei,QIANG Wei,HE Xueli

2018, 38(1):  73-82.  DOI: 10.3969/j.issn.1000-1565.2018.01.012

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In order to elucidate the colonization patterns of dark septate endophytes(DSE)in roots of different plants and its correlation with soil factors in extreme arid desert environment, the samples of soil and roots of Ephedra przewaii, Reaumuria soongorica, Sympegma regelii, Nitraria sphaerocarpa and Salsola passerina were collected in Anxi, Gansu Province, July 2016.The results showed that DSE could infect the roots of five desert plants, and formed typical melanized septate hyphae and microsclerotia; Soil acid phosphatase, urease, total extractable glomalin and soil humidity significantly affected the DSE colonization intensity; The plant species had a significantly direct effect on the total and hyphal colonization of DSE, and indirectly affected the DSE colonization by the soil temperature and humidity, urease, acid phosphatase. The results supported the conclusion that plant species and soil factors were important factors affecting DSE colonization.- DOI:10.3969/j.issn.1000-1565.2018.01.012极旱荒漠植物深色有隔内生真菌定殖及其与土壤因子的相关性葛佳丽,张琳琳,李欣玫,强薇,贺学礼(河北大学 生命科学学院,河北 保定 071002)摘 要:为了探明荒漠环境深色有隔内生真菌(dark septate endophytes, DSE)生态分布和定殖状况,于2016年7月从甘肃安西极旱荒漠国家自然保护区采集膜果麻黄(Ephedra przewaii)、红砂(Reaumuria soongorica)、合头草(Sympegma regelii)、泡泡刺(Nitraria sphaerocarpa)和珍珠猪毛菜(Salsola passerina)等5种植物根际和根围0~30 cm土层土壤样品和根样,研究了不同荒漠植物DSE定殖及其与土壤因子的相关性. 结果发现:5种荒漠植物根系均能被DSE侵染,DSE分布和定殖具有明显的空间异质性,并与土壤因子密切相关;不同根系来源样品DSE定殖率与土壤湿度、脲酶存在极显著负相关性;土壤湿度、酸性磷酸酶和脲酶显著影响DSE定殖强度;植物种类对DSE菌丝定殖率和总定殖率具有显著直接影响,并通过土壤温湿度、脲酶、酸性磷酸酶间接影响DSE定殖.可见,植物种类和土壤因子是影响DSE定殖的重要因素.关键词:深色有隔内生真菌;生态分布;植物种类;极旱荒漠环境中图分类号:Q934 文献标志码:A 文章编号:1000-1565(2018)01-0073-10Colonization of dark septate endophytes in roots of different plantsand its correlation with soil factors in extreme arid desert environmentGE Jiali,ZHANG Linlin,LI Xinmei,QIANG Wei,HE Xueli(College of Life Sciences,Hebei University, Baoding 071002,China)Abstract: In order to elucidate the colonization patterns of dark septate endophytes(DSE)in roots of different plants and its correlation with soil factors in extreme arid desert environment, the samples of soil and roots of Ephedra przewaii, Reaumuria soongorica, Sympegma regelii, Nitraria sphaerocarpa and Salsola passerina were collected in Anxi, Gansu Province, July 2016.The results showed that DSE could infect the roots of five desert plants, and formed typical melanized septate hyphae and microsclerotia; Soil acid phosphatase, urease, total extractable glomalin and soil humidity significantly affected the DSE colonization intensity; The plant species had a significantly direct effect on the total and hyphal colonization of DSE, and indirectly affected the DSE colonization by the soil temperature and humidity, urease, acid phosphatase. The results supported the conclusion that plant species and soil factors were important factors affecting DSE colonization.- 收稿日期:2017-03-21 基金项目:国家自然科学基金资助项目(31470533) 第一作者:葛佳丽(1991—),女,山西临汾人,河北大学在读硕士研究生.E-mail:1728099876@qq.com 通信作者:贺学礼(1963—),男,陕西蒲城人,河北大学教授,博士生导师,主要从事植物生态学和土壤生态学研究.E-mail: xuelh1256@aliyun.com第1期葛佳丽等:极旱荒漠植物深色有隔内生真菌定殖及其与土壤因子的相关性

Correlation of beak morphology and song features in Dusky Warbler(Phylloscopus fuscatus)

WANG Jiaojiao, LIU Jianping, LIU Bingyang, HOU Jianhua

2018, 38(1):  83-88.  DOI: 10.3969/j.issn.1000-1565.2018.01.013

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To study the relationship between beak morphology and sound frequency, from 5 July to 10 August 2015, we recorded 33 dusky warbler(Phylloscopus fuscatus)songs between 5:00 a.m. and 10:00 a.m. in fine days(no wind, no rain)in the breeding season. The beak morphology parameters and the sound characteristics were measured. The results showed that: there was no relationship between high frequency, low frequency, max frequency and bandwidth of songs and culmen or beak gape; culmen was found to correlate negatively with low frequency of trill, however, there was no correlation between the culmen and high frequency, the max frequency or the bandwidth; beak gape was not significantly related to the trill traits. The results of this study demonstrate that culmen can provide information about the low frequency of trill, and vocal tract can affect the characteristics of sound.

Expectation reduction-based fuzzy nonlinear regression

WANG Xizhao, ZHAO Shixin

2018, 38(1):  89-98.  DOI: 10.3969/j.issn.1000-1565.2018.01.014

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Inspired by the idea of reduction in 2-fuzzy theory, an expectation reduction-based fuzzy nonlinear regression model is proposed to deal with triangular fuzzy number inputs and outputs.In this model the triangular fuzzy inputs and outputs are firstly replaced by their expectations, then the data can be trained with classical random weight network.Finally the crisp real outputs are recover to triangular fuzzy outputs by using a width matrix of original target triangular fuzzy outputs.The experiment results show that the proposed model gives higher learning accuracy and better generalization.

Empirical study on a prediction model for the unemployment rate of college graduates based on principal component analysis and BP neural network

LIU Zhao

2018, 38(1):  99-104.  DOI: 10.3969/j.issn.1000-1565.2018.01.015

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A prediction model for the unemployment rate of college graduates was developed based on principal component analysis and BP neural network, followed by analyzing the unemployment data of college graduates in Hebei province in the years 1995—2016.The results show that the model can effectively reflect the variation tendency of the unemployment rate of college graduates in Hebei province and the prediction accuracy of the model is higher than that of the model only based on BP neural network.

Composite control method for three-phase four-leg inverter

YAN Feng,HUANG Lihua,WANG Hui,TANTAI Xiaohan,HAN Jiao,YU Wei

2018, 38(1):  105-112.  DOI: 10.3969/j.issn.1000-1565.2018.01.016

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In the application of wind, solar and other clean energy in the grid,inverter is indispensable.Harmonic wave and unbalanced load affect the output of the inverter, the performance of the inverter control strategy directly affects the power quality.By combining proportional integral(PI), vector resonance(VR)control and repetitive control(RC),the compound control method is proposed.PI restrain the interference of DC component, the VR controller suppresses the low harmonics.RC suppresses the higher harmonics and reduce the static error, and improve the waveform qualities of converter output voltage and current.The simulation results verify the effectiveness of the proposed control method.