Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro

doi:10.3969/j.issn.1000-1565.2016.02.013

Abstract

Abstract: The effects of different concentrations of uric acid on hemorheology were investigated in vitro.The erythrocytes were suspended in 300,600,1 200 μmol/L uric acid for 2 h and the hemorheological parameters were measured.The membrane protein composition was also analyzed by SDS-PAGE.We found that the integrated deformation index and relax index were reduced in a dose-dependent manner.The hemolysis rate increased.As compared with the control group,the content of ankyrin and band 3 proteins showed a decrease trend.These results were statistically significant in the 1 200 μmol/L group.The results suggest that high concentrations of uric acid can have significant damaging effects on erythrocyte membrane proteins,which is an independent factor affecting the hemorheological properties of erythrocytes.

Key words: uric acid, erythrocyte, hemorheology, membrane proteins

CLC Number:

LI Kuihua,LI Lu,YAN Peng,TONG Xiaobo,XIE Lide. Effects of different concentrations of uric acid on erythrocyte hemorheology in vitro[J]. Journal of Hebei University (Natural Science Edition), 2016, 36(2): 184-188.

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