Journal of Hebei University(Natural Science Edition) >

2020 , Vol. 40 >Issue 2: 171 - 175

DOI: https://doi.org/10.3969/j.issn.1000-1565.2020.02.009

Direct shoot regeneration in vitro for Atractylodes macrocephala using epicotyl and radicle as explants

  • LIANG Yuling ,
  • XIANYU Liangyan ,
  • LIU Wenwen ,
  • ZHANG Yuanyuan
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  • 1.College of Life Sciences, Hebei University, Baoding 071002, China; 2.Key Disciplines of Biotechnology Engineering Hebei Province, Baoding 071002, China

Received date: 2019-07-13

  Online published: 2020-03-25

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Abstract

For establishing the high efficiency regeneration system in vitro of Atractylodes macrocephala Koidz.,the effects of explant types and concentrations of plant growth regulators on the direct shoots regeneration for A.macrocephala were examined.The results indicated that shoots were directly induced from radicle and epicotyl explants without callus induction.The appropriate medium for shoot regeneration was MS + TDZ 1.5 mg/L + NAA 0.2 mg/L.With this regeneration system,91.18% of epicotyl explants produced an average of 13 elongated shoots per initial explant inoculated.The rooting efficiency was up to 66.66% on the 1/2 MS + IBA 0.5 mg/L.The regeneration system for A.macrocephala provided reliable technical support for plant genetic engineering quality improvement,industrial seeding,and virus-free of A.macrocephala.

Key words： Atractylodes macrocephalaKoidz.; radicle; epicotyl; direct shoot regeneration

Cite this article

LIANG Yuling , XIANYU Liangyan , LIU Wenwen , ZHANG Yuanyuan . Direct shoot regeneration in vitro for Atractylodes macrocephala using epicotyl and radicle as explants[J]. Journal of Hebei University(Natural Science Edition), 2020 , 40(2) : 171 -175 . DOI: 10.3969/j.issn.1000-1565.2020.02.009

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