BALB/c female mice were kept in a multifunctional radon chamber for continuous radon inhalation in order to explore the damage and immune cell reaction of lung tissue after radon exposure. Respiratory function was evaluated at 15 d, 30 d and 60 d after exposure. Peripheral blood was collected for detection of blood cells and blood biochemical indexes, and lung tissue was collected for pathological observation and fine-activation of immune cells such as T lymphocytes and their subsets was determined. The results showed that the radon exposed mice showed obvious toxic reactions such as weight loss, leukopenia, electrolyte metabolism disorder and lipid metabolism abnormality. Abnormal respiratory function and lung tissue lesions of different degrees were observed, and the early inflammatory reaction of lung tissue was obvious, mainly manifested in the imbalance and activation of T lymphocyte subsets. This indicates that there are obvious adverse reactions in mice exposed to radon. This study can provide experimental- DOI:10.3969/j.issn.1000-1565.2024.02.006氡暴露小鼠损伤病理及肺组织免疫细胞反应王美玉1,刘慧蒙2,王志鑫2,郭浩鑫3,王易龙2,朱茂祥1,2,3(1.河北大学 生命科学学院,河北 保定 071002;2.军事医学研究院 辐射医学研究所,北京市放射生物学重点实验室,北京 100850;3.南华大学 公共卫生学院,湖南 衡阳 421001)摘 要:为探索小鼠氡暴露后不同时间机体损伤及肺组织免疫细胞反应,将BALB/c雌性小鼠饲养于多功能氡室进行连续氡吸入.分别在暴露后15、30、60 d进行呼吸功能评价,采集外周血进行血细胞和血液生化指标检测,取肺组织进行病理形态观察,取T淋巴细胞及其亚群等免疫细胞活化测定.结果显示:氡暴露小鼠出现体质量下降、白细胞减少、电解质代谢紊乱、脂质代谢异常等明显毒性反应,观察到呼吸功能异常及肺组织不同程度病变,且早期肺组织炎性反应明显,主要表现在T淋巴细胞亚群失衡及其活化.这说明氡暴露小鼠存在明显机体不良反应.本研究可为氡吸入的危害评价与医学防护研究提供实验依据.关键词:氡暴露;肺损伤病理;呼吸功能;免疫细胞反应中图分类号:R818.8 文献标志码:A 文章编号:1000-1565(2024)02-0156-08Injury pathology and immune cell response of lung tissue in radon exposed miceWANG Meiyu1, LIU Huimeng2, WANG Zhixin2, GUO Haoxin3, WANG Yilong2, ZHU Maoxiang1,2,3(1. College of Life Sciences, Hebei University, Baoding 071002, China; 2. Beijing Key Laboratory of Radiobiology, Institute of Radiation Medicine, Academy of Military Medicine Sciences, Beijing 100850, China; 3. School of Public Health, University of South China, Hengyang 421001, China)Abstract: BALB/c female mice were kept in a multifunctional radon chamber for continuous radon inhalation in order to explore the damage and immune cell reaction of lung tissue after radon exposure. Respiratory function was evaluated at 15 d, 30 d and 60 d after exposure. Peripheral blood was collected for detection of blood cells and blood biochemical indexes, and lung tissue was collected for pathological observation and fine-activation of immune cells such as T lymphocytes and their subsets was determined. The results showed that the radon exposed mice showed obvious toxic reactions such as weight loss, leukopenia, electrolyte metabolism disorder and lipid metabolism abnormality. Abnormal respiratory function and lung tissue lesions of different degrees were observed, and the early inflammatory reaction of lung tissue was obvious, mainly manifested in the imbalance and activation of T lymphocyte subsets. This indicates that there are obvious adverse reactions in mice exposed to radon. This study can provide experimental- 收稿日期:2022-11-14;修回日期:2022-12-30 基金项目:国家自然科学基金资助项目(81673095) 第一作者:王美玉(1998—),女,河北大学在读硕士研究生,主要从事放射性肺损伤研究.E-mail:18630596228@163.com 通信作者:朱茂祥(1966—),男,军事医学研究院教授,主要从事放射毒理与医学防护研究.E-mail:zhumx2016@126.com第2期王美玉等:氡暴露小鼠损伤病理及肺组织免疫细胞反应basis for the hazard assessment and medical protection research of radon inhalation.
WANG Meiyu
,
LIU Huimeng
,
WANG Zhixin
,
GUO Haoxin
,
WANG Yilong
,
ZHU Maoxiang
. Injury pathology and immune cell response of lung tissue in radon exposed mice[J]. Journal of Hebei University(Natural Science Edition), 2024
, 44(2)
: 156
-163
.
DOI: 10.3969/j.issn.1000-1565.2024.02.006
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