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25 November 2022, Volume 42 Issue 6

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First cohomology of Lie superalgebra P^~(2)with coefficients to Kac modules

ZHANG Zilu, SUN Liping, WANG Shujuan

2022, 42(6):  561-564.  DOI: 10.3969/j.issn.1000-1565.2022.06.001

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Over a field of characteristic p≥3,firstly,according to the Z-degree structure of P^~(2)and the standard Cartan decomposition of the 0-degree P^~(2)₀,for a given weight λ and the maximal vector v_λ, finite-dimensional irreducible modules M(λ)of P^~(2)₀ are constructed, and then its Kac structure is given. Secondly, using the definition of the first cohomology group, the first cohomology group from P^~(2)to its Kac module is transformed into the weight-derivation of the non-inner derivation of P^~(2)to Kac module. Finally, the first cohomology group of P^~(2)with coefficients to a class of Kac modules is determined.

Zeroth cohomology from W(2)to Kac modules

DING Yazhou, WANG Shujuan

2022, 42(6):  565-568.  DOI: 10.3969/j.issn.1000-1565.2022.06.002

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Over a field of characteristic p≥3,this paper determines the 0-th cohomology from Witt-type Modular Lie superalgebra W(2)to all restricted and non-restricted Kac modules K_S(λ).We show that the 0-th cohomology spaces of W(2)with coefficients in K_S(λ)is 1-dimensional if S=0, λ=(0,2),and the 0-th cohomology spaces of W(2)with coefficients in K_S(λ)is 0-dimensional if S≠0 or λ≠(0,2).

Design of integrated machine for potato harvesting and residual film collecting in complex terrain

MA Yonglong, YANG Shuming, LI Maoqiang, WANG Qian, KE Zerong

2022, 42(6):  569-579.  DOI: 10.3969/j.issn.1000-1565.2022.06.003

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Aiming at the complex terrain of southern Ningxia, a potato harvesting and residual film collecting integrated machine is designed for complex terrain, which can complete potato digging and residual film recovery in one operation. The key components of the prototype were designed and selected to determine the structure and parameters of the digging blade, the S-shaped soil-potato dither conveyor device and the pneumatic residual film recovery mechanism. With ANSYS fluent, using two design parameters, the number of blades of the impeller and the rotation speed of the impeller, the comparison and analysis on the full pressure cloud diagram, velocity cloud diagram and velocity vector diagram of the internal flow field of the residual film collecting mechanism are carried out.When the number of blades of the impeller is 16 and the impeller rotation speed is 1 120r/min, the residual film collecting mechanism has the best effect on collecting residual film. The field experiment results showed that the soil-potato separation effect of this structure is significant. The residual film can be continuously sucked by the residual film collecting mechanism and transported to the residual film collecting box. The potato exposure rate was 98.6%,the potato injury rate was 2.92% and the collecting rate of residual film was 90.77%. The experiment results meets the design requirements of technical specifications. - DOI:10.3969/j.issn.1000-1565.2022.06.003复杂地形马铃薯收获及残膜回收一体机设计马永龙¹,杨术明¹,李茂强²,王乾¹,柯泽荣¹(1.宁夏大学 机械工程学院,宁夏 银川 750021;2.北方民族大学 机电工程学院,宁夏 银川 750030)摘 要:针对宁夏南部山区复杂地形,设计了复杂地形马铃薯收获及残膜回收一体机,一次作业能够完成马铃薯挖掘和残膜回收.通过样机设计及部件选型,确定挖掘铲、S型土薯抖动升运链及气力式残膜回收机构等关键部件的结构及参数.运用ANSYS Fluent,以收膜机构叶轮的叶片数和叶轮旋转速度为设计参数,对收膜机构内部流场全压云图、速度云图和速度矢量图进行对比分析,确定叶轮的叶片数为16片、叶轮转速为1 120 r/min时,收膜机构的残膜收集效果最佳.田间试验结果表明,该机型土薯分离效果显著,残膜能够连续地被收膜机构吸入并输送至集膜箱,明薯率为98.6%,伤薯率为2.92%,残膜拾挣率90.77%.试验指标均达到国家和行业标准要求.关键词:复杂地形;马铃薯收获;残膜;一体机;收膜机构中图分类号:S225.71 文献标志码:A 文章编号:1000-1565(2022)06-0569-11Design of integrated machine for potato harvestingand residual film collecting in complex terrainMA Yonglong¹, YANG Shuming¹, LI Maoqiang², WANG Qian¹, KE Zerong¹(1.College of Mechanical Engineering, Ningxia University, Yinchuan 750021,China; 2.College of Mechanical and Electrical Engineering, North Minzu University, Yinchuan 750030,China)Abstract: Aiming at the complex terrain of southern Ningxia, a potato harvesting and residual film collecting integrated machine is designed for complex terrain, which can complete potato digging and residual film recovery in one operation. The key components of the prototype were designed and selected to determine the structure and parameters of the digging blade, the S-shaped soil-potato dither conveyor device and the pneumatic residual film recovery mechanism. With ANSYS fluent, using two design parameters, the number of blades of the impeller and the rotation speed of the impeller, the comparison and analysis on the full pressure cloud diagram, velocity cloud diagram and velocity vector diagram of the internal flow field of the residual film collecting mechanism are carried out.When the number of blades of the impeller is 16 and the impeller rotation speed is 1 120r/min, the residual film collecting mechanism has the best effect on collecting residual film. The field experiment results showed that the soil-potato separation effect of this structure is significant. The residual film can be continuously sucked by the residual film collecting mechanism and transported to the residual film collecting box. The potato exposure rate was 98.6%,the potato injury rate was 2.92% and the collecting rate of residual film was 90.77%. The experiment results meets the design requirements of technical specifications. - 收稿日期:2022-04-09 基金项目:宁夏回族自治区重点研发计划重大项目(2019BFF02003) 第一作者:马永龙(1987—),男,宁夏吴忠人,回族,宁夏大学在读硕士研究生.E-mail:18795274127@163.com 通信作者:杨术明(1974—),男,宁夏吴忠人,回族,宁夏大学教授,博士,主要从事农业装备自动化与智能化方向研究.E-mail:shmyang@126.com第6期马永龙等:复杂地形马铃薯收获及残膜回收一体机设计

Nonequivalent cumulative damage model

SUN Zhenduo, LYU Songfeng, HOU Dongbo, ZHANG Mingyang

2022, 42(6):  580-588.  DOI: 10.3969/j.issn.1000-1565.2022.06.004

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The fatigue test of No.45 steel under variable amplitude loading was carried out. Based on the experimental results, the prediction accuracy of Miner model, Manson-Halford model, Cortan-Dolan model and YE Duyi model is compared and analyzed. Based on Miner model and Manson-Halford model, two new nonequivalent cumulative damage models are constructed respectively with the principle of nonequivalent cumulative damage. The results show that, the prediction accuracy of Manson-Halford model is better than the other three models, and the prediction results of new nonequivalent cumulative damage models constructed in this paper are better than that of Manson-Halford model, and its calculation mode is relatively simple and suitable for engineering application.

Synthesis and thermometric performance of NaY₉(SiO₄)₆O₂:〓 Bi³⁺,Eu³⁺phosphor

FU Nian, YANG Zihan, MA Lianhua, WANG Chunhao, TU Yingying, LU Fachun

2022, 42(6):  589-598.  DOI: 10.3969/j.issn.1000-1565.2022.06.005

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To search probe phosphors with better performance temperature, using the ratio method of the fluorescence intensity, the thermosensitive performance of the NaY_9-x-y(SiO₄)₆O₂:xBi³⁺,yEu³⁺ phosphor has been studied. The results show the efficient energy transfer from Bi³⁺ to Eu³⁺ can be observed. With the excitation of Bi³⁺, the double emission spectra of Bi³⁺ and Eu³⁺ can be acquired. With the increase of the temperature, the fluorescence intensity quenching speed of Eu³⁺ is slower than that of Bi³⁺. Based on the temperature dependent fluorescence intensity ratio of Bi³⁺ to Eu³⁺, the relative sensitivity(S_r)values of the NaY_6.192(SiO₄)₆O₂:0.108Bi³⁺,2.7Eu³⁺ probe phosphor at the temperature range of 300 to 450 K have been calculated. The maximum(S_r)is 1.51 %·K^-1, which is much better than the suggested value of 0.3 %·K^-1 for the practical applications. The temperature probe phosphor in this work showed the potential in the temperature sensitivity fields.

Atomic oxygen concentration of an atmospheric pressure plasma jet investigated by actinometry

XU Huimin, TANG Xiao, WU Jiacun, CHEN Junyu, ZHAO Na, JIA Pengying

2022, 42(6):  599-604.  DOI: 10.3969/j.issn.1000-1565.2022.06.006

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Among the various active species produced in plasma jet, oxygen atom is a kind of oxidants with high chemical reactivity. Moreover, it is a fundamental specie to generate other reactive oxygen species. Therefore, it is very important for efficiency improvement in determining oxygen atom concentration in plasma jet. Aim to this status, a plasma plume with a solid morphology is generated downstream of a plasma jet in a single-electrode configuration. Optical emission spectrum is collected from the plasma jet by a high-resolution spectrometer, which presents that active species including oxygen atom are abundant in the plasma plume. Moreover, intensity ratio of O I(777.4 nm)and Ar I(750.4 nm)is investigated to obtain the variation of oxygen atom concentration by actinometry. Experimental results indicate- DOI:10.3969/j.issn.1000-1565.2022.06.006光化线强度法研究大气压等离子体射流的氧原子浓度许慧敏^1,2,唐潇¹,武珈存³,陈俊宇³,赵娜³,贾鹏英³(1.河北金融学院 基础教学部, 河北 保定 071051;2.华北电力大学 电力工程系,河北 保定 071003;3.河北大学 物理科学与技术学院,河北 保定 071002)摘 要:等离子体射流产生的众多活性粒子中,氧原子是化学活性非常强的氧化剂,也是生成其他含氧活性粒子的基础,因此确定氧原子浓度及其时空分布对提高等离子体射流的应用效率具有重要意义.针对于此,本工作利用单电极等离子体射流产生了实心结构的等离子体羽,利用高分辨光谱仪采集了放电的发射光谱.结果表明,等离子体羽中确实含有氧原子等活性粒子.采用光化线强度法通过比较氧原子谱线(777.4 nm)和氩原子谱线(750.4 nm)的强度比研究了等离子体中氧原子浓度.结果表明:等离子体羽中的氧原子浓度随着距离喷口距离的增加先减小后增大;固定其他实验参数的情况下,氧原子浓度随着外加电压峰值和工作气体流量的增加而增大;当工作气体中掺入空气后,等离子体羽中氧原子浓度随着空气体积分数的增加先增大后减小.结合放电机制,对以上实验现象进行了定性分析,所得结果对于大气压等离子体射流的应用具有重要意义.关键词:等离子体射流;发射光谱;光化线强度;氧原子浓度中图分类号:O433.1 文献标志码:A 文章编号:1000-1565(2022)06-0599-06Atomic oxygen concentration of an atmospheric pressure plasma jet investigated by actinometryXU Huimin^1,2, TANG Xiao¹, WU Jiacun³, CHEN Junyu³, ZHAO Na³, JIA Pengying³(1. Department of Fundamental Teaching, Hebei Finance University, Baoding 071051, China2. Department of Electrical Engineering, North China Electric Power University, Baoding 071003, China3. College of Physics Science and Technology, Hebei University, Baoding 071002, China)Abstract: Among the various active species produced in plasma jet, oxygen atom is a kind of oxidants with high chemical reactivity. Moreover, it is a fundamental specie to generate other reactive oxygen species. Therefore, it is very important for efficiency improvement in determining oxygen atom concentration in plasma jet. Aim to this status, a plasma plume with a solid morphology is generated downstream of a plasma jet in a single-electrode configuration. Optical emission spectrum is collected from the plasma jet by a high-resolution spectrometer, which presents that active species including oxygen atom are abundant in the plasma plume. Moreover, intensity ratio of O I(777.4 nm)and Ar I(750.4 nm)is investigated to obtain the variation of oxygen atom concentration by actinometry. Experimental results indicate- 收稿日期:2022-01-10 基金项目:国家自然科学基金资助项目(51977057;11875121);河北省自然科学基金资助项目(A2015201199; A2015201092; A2016201042);河北大学研究生创新项目(CXZZSS2020006) 第一作者:许慧敏(1977—),女,河北博野人,河北金融学院副教授,主要从事气体放电研究方向研究.E-mail:xuhuimin@126.com第6期许慧敏等:光化线强度法研究大气压等离子体射流的氧原子浓度that oxygen atom concentration in plasma plume first decreases and then increases with increasing distance from the jet nozzle. Furthermore, it increases with increasing peak value of applied voltage or gas flow rate. After mixing trace air into the working gas, it is found that oxygen atom concentration first increases and then decreases with increasing air content into the working gas. Optimal concentration of oxygen atom appears at about 0.02% air content. After considering discharge regime, the experimental phenomena mentioned above have been analyzed qualitatively. All these results are of great importance for the application of atmospheric pressure plasma jet.

Discrimination of adulteration of edible vegetable oil based on mid-infrared spectroscopy model

LI Yankun, XU Dongqing

2022, 42(6):  605-610.  DOI: 10.3969/j.issn.1000-1565.2022.06.007

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A chemometric model based on mid-infrared spectroscopy was developed to study the adulteration of edible vegetable oil. The mid-infrared spectra of commercial edible oil and adulterated edible oil with different mass concentrations were obtained and analyzed by spectral preprocessing combined with pattern recognition.The results show that unsupervised methods including principal component analysis and nonnegative matrix factorization do not perform accurately; supervised partial least squares discriminant analysis has high discrimination accuracy; the second derivative-uncorrelated linear discriminant analysis(SD-ULDA)maximizes the sample class spacing and minimizes the class spacing as much as possible, and the sensitivity and specificity for the identification of adulterated samples are 100%.A new strategy of characteristic wavelength(variables)screening according to ‘load capacity’ of variables is proposed, and the change of recognition accuracy of SD-ULDA model with reserved wavelength was investigated. Simultaneously, the adulteration proportion was accurately predicted. It provides a theoretical basis for constructing a high-efficiency mid-infrared spectrum edible oil adulteration- DOI:10.3969/j.issn.1000-1565.2022.06.007基于中红外光谱模型对食用植物油掺伪的判别李艳坤,许东情(华北电力大学(保定)环境科学与工程系,河北省燃煤电站烟气多污染物协同控制重点实验室,河北 保定 071003)摘 要:开展了基于中红外光谱的化学计量学模型对食用植物油掺伪的研究.通过获得市售食用油和以不同质量浓度掺伪食用油的中红外光谱,采用光谱预处理结合模式识别方法进行综合解析,使样本类间距最大化,类内距最小化,结果发现:无监督聚类包括主成分分析、非负矩阵分解均无法准确鉴别;有监督偏最小二乘判别分析的鉴别准确度较高;而二阶导数-非相关线性判别分析(SD-ULDA)对掺伪样本识别的灵敏度和特异度均达到100%.提出依据变量“负荷量”筛选特征波长(变量)新策略,考察了SD-ULDA模型识别准确率随保留波长的变化,同时准确地预测出掺伪比例,为构建高效中红外光谱食用油掺伪判别模型提供理论基础,为提高油脂品质的检测技术提供方法借鉴.关键词:中红外光谱;食用油;掺伪鉴别;非相关线性判别分析;波长筛选中图分类号:O433.4 文献标志码:A 文章编号:1000-1565(2022)06-0605-06Discrimination of adulteration of edible vegetable oil based on mid-infrared spectroscopy modelLI Yankun, XU Dongqing(Key Laboratory for Collaborative Control of Multi Pollutants in Flue Gas of Coal-fired Power Plants, Department of Environment Science and Engineering, North China Electric Power University, Baoding 071003, China)Abstract: A chemometric model based on mid-infrared spectroscopy was developed to study the adulteration of edible vegetable oil. The mid-infrared spectra of commercial edible oil and adulterated edible oil with different mass concentrations were obtained and analyzed by spectral preprocessing combined with pattern recognition.The results show that unsupervised methods including principal component analysis and nonnegative matrix factorization do not perform accurately; supervised partial least squares discriminant analysis has high discrimination accuracy; the second derivative-uncorrelated linear discriminant analysis(SD-ULDA)maximizes the sample class spacing and minimizes the class spacing as much as possible, and the sensitivity and specificity for the identification of adulterated samples are 100%.A new strategy of characteristic wavelength(variables)screening according to ‘load capacity’ of variables is proposed, and the change of recognition accuracy of SD-ULDA model with reserved wavelength was investigated. Simultaneously, the adulteration proportion was accurately predicted. It provides a theoretical basis for constructing a high-efficiency mid-infrared spectrum edible oil adulteration- 收稿日期:2022-03-03 基金项目:中央高校基本科研业务费资助项目(2017MS135) 第一作者:李艳坤(1977—),女,河北邯郸人,华北电力大学副教授,博士,主要从事分析化学、化学信息学的研究.E-mail:liyankun_ncepu@foxmail.com第6期李艳坤等:基于中红外光谱模型对食用植物油掺伪的判别discrimination model and provides a reference method for improving the detection technology of oil quality.

Synthesis and luminescence mechanism of Mn-doped CsPbCl₃/Cs₄PbCl₆ composites

YANG Xiangce, ZHANG Heng, LIU Chenhui, ZHU Mengxuan, WANG Fenghe, GUO Yingnan

2022, 42(6):  611-617.  DOI: 10.3969/j.issn.1000-1565.2022.06.008

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In order to develope synthesis methods suitable for industrial production. In this work, Mn-doped CsPbCl₃/Cs₄PbCl₆(CCM)composites were synthesized by ultrasonic method and their luminescence mechanism were investigated. CCM has a bright red emission under near-ultraviolet excitation, and there were three emission peaks located at 606 nm(Mn²⁺), 414 nm(CsPbCl₃), and 351 nm(Cs₄PbCl₆). Energy transfer from CsPbCl₃ to Mn²⁺ and from Cs₄PbCl₆ to Mn²⁺ were presented in CCM. By varying the MnCl₂/PbCl₂ ratio in the precursor, the passivation of Cs₄PbCl₆ on CsPbCl₃, the energy transfer from CsPbCl₃ to Mn²⁺ and the red-light emission intensity of Mn²⁺ were modulated.

Determination of thiolidazine hydrochloride in chicken by HPLC

HAN Xiaoli, LIU Guangxing, LI Yiqing, ZHENG Jiayan, WANG Tingxin

2022, 42(6):  618-624.  DOI: 10.3969/j.issn.1000-1565.2022.06.009

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To establish an HPLC method for the determination of thiolidazine hydrochloride in chicken,the best detection wavelength of thiolidazine hydrochloride was determined by high performance liquid chromatograph(HPLC)with a diode array detector.The samples were extracted with ethyl acetate as solvent, and the fat was removed with n-hexane, concentrated by nitrogen blowing, then dissolved with acetonitrile, purified with 60 mg HLB solid phase extraction column, and finally filtered with 0.22 μm organic membrane. The liquid chromatographic conditions were as follows: mobile phase consisted of acetonitrile-0.1% formic acid aqueous solution(V∶V=35∶65), the flow rate was 1 mL/min, and diode array detector was used for detection. Quantitative analysis was carried out by the external standard method. The results show that the linearity of the method is good in the range of 0.1—10.0 μg/mL. The limit of detection was 1.2 μg/kg, the limit of quantitation was 4.0 μg/kg, and the recoveries were 8.96%—98.58%. The HPLC method established in this study for the determination of thiolidazine hydrochloride in chicken has the advantages of good precision, convenient operation and high recovery.It can provide a technical method for the detection of tranquilizers in animal food.

CTCF promotes the growth, migration and invasion of cholangiocarcinoma cells by inhibiting the p53 signaling pathway

YAN Han, SHI Peipei, WANG Yahui, GAO Chengming, ZHOU Gangqiao

2022, 42(6):  625-635.  DOI: 10.3969/j.issn.1000-1565.2022.06.010

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The study aims to explore the functions and potential molecular mechanisms of CCCTC-binding factor(CTCF)in cholangiocarcinoma(CCA)cell lines. The CCA cell lines with stable overexpression or knockdown of CTCF were obtained by lentivirus infection. Western blotting(Wb)assays were used to detect the CTCF protein expression levels.The cell growth was detected by CCK-8 assays and plated cell clone formation assays. Cell migration and invasion was detected by Transwell. Mann-Whitney- DOI:10.3969/j.issn.1000-1565.2022.06.010CTCF通过抑制p53信号通路促进胆管癌细胞的生长、迁移和侵袭闫晗¹,石培培²,王亚会³,高成明³,周钢桥^1,3(1.河北大学 化学与环境科学学院,河北 保定 071002; 2.河北大学 生命科学学院,河北 保定 071002;3.军事科学院军事医学研究院 辐射医学研究所,北京 100850)摘 要:为了探索CCCTC结合因子(CCCTC-binding factor, CTCF)对人胆管癌细胞的生长、迁移和侵袭能力的影响及其潜在的分子作用机制,利用慢病毒感染法获得稳定过表达或敲低CTCF的胆管癌细胞系,采用蛋白质免疫印迹法(Western blotting, Wb)检测CTCF蛋白表达水平,采用Cell Counting Kit-8(CCK-8)法和克隆形成实验检测细胞生长情况,采用Transwell小室实验检测细胞迁移和侵袭能力,采用GraphPad软件(v6)的Mann-Whitney U检验分析CTCF基因在癌和癌旁组织间的mRNA差异表达.结果显示:过表达CTCF可显著促进HCCC-9810和RBE胆管癌细胞的生长、迁移和侵袭能力;敲低CTCF呈现相反的表型.通过通路富集分析发现:CTCF的表达水平在胆管癌中与p53信号通路活性显著负相关,过表达CTCF可显著下调p53及其靶基因p21的mRNA和蛋白表达水平,而敲低CTCF则显著促进p53和p21的mRNA和蛋白表达水平.综上,本研究揭示CTCF可能通过抑制p53信号通路而促进胆管癌细胞生长、迁移和侵袭而发挥促癌基因的功能.关键词:胆管癌;CCCTC结合因子(CTCF);细胞生长;细胞迁移;细胞侵袭;p53通路中图分类号:R735.8 文献标志码:A 文章编号:1000-1565(2022)06-0625-11CTCF promotes the growth, migration and invasion of cholangiocarcinoma cells by inhibiting the p53 signaling pathwayYAN Han¹, SHI Peipei², WANG Yahui³, GAO Chengming³, ZHOU Gangqiao^1,3(1.College of Chemistry and Environmental Science, Hebei University, Baoding 071002, China; 2.College of Life Sciences, Hebei University, Baoding 071002, China; 3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China)Abstract: The study aims to explore the functions and potential molecular mechanisms of CCCTC-binding factor(CTCF)in cholangiocarcinoma(CCA)cell lines. The CCA cell lines with stable overexpression or knockdown of CTCF were obtained by lentivirus infection. Western blotting(Wb)assays were used to detect the CTCF protein expression levels.The cell growth was detected by CCK-8 assays and plated cell clone formation assays. Cell migration and invasion was detected by Transwell. Mann-Whitney- 收稿日期:2022-04-13 基金项目:国家自然科学青年基金资助项目(82002573) 第一作者:闫晗(1997—),女,河南焦作人,河北大学硕士研究生,主要从事遗传学和整合组学研究. E-mail:yanhancinada@126.com 通信作者:周钢桥(1972—),男,北京人,军事科学院军事医学研究院研究员,主要从事遗传学和整合组学研究.E-mail: zhougq114@126.com第6期闫晗等:CTCF通过抑制p53信号通路促进胆管癌细胞的生长、迁移和侵袭U test of GraphPad software(v6)was used to analyze the mRNA differential expression of CTCF gene between cancer and adjacent tissues. The results show that overexpression of CTCF significantly promoted the growth, migration and invasion of HCCC-9810 and RBE cells, whereas knockdown of CTCF exhibited the opposite effects. Through pathway enrichment analysis, it was found that the expression level of CTCF was significantly negatively correlated with the activity of p53 signaling pathway in cholangiocarcinoma.Overexpression of CTCF significantly reduced the expression of p53 and its target gene p21, while knockdown of CTCF showed the opposite effects. In conclusion, this study revealed that CTCF function as an oncogene by inhibiting the p53 signaling pathway to promote the growth, migration and invasion of CCA cells.

Characters of Platyscelis(Platyscelis)brevis and Platyscelis(Platyscelis)subcordata with updated distribution(Coleoptera: Tenebrionidae: Platyscelidini)

TIAN Ying, BAI Xinglong

2022, 42(6):  636-641.  DOI: 10.3969/j.issn.1000-1565.2022.06.011

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Platyscelis(Platyscelis)brevis Baudi di Selve, 1876 and P.(P.)subcordata Seidlitz, 1893 are redescribed, characters and habitus of the males are presented. Geographical distributions of them are updated based on the examined materials: P. brevis distributes in China(Beijing, Hebei, Heilongjiang, Inner Mongolia, Jilin, Liaoning, Ningxia, Shandong, Shanxi), Mongolia and Russia(Siberia); P. subcordata distributes in Heilongjiang, Liaoning, and Shandong of China.

Optimization of fluorescence detection of extracellular enzyme activities in rhizosphere soil

TANG Lianfa, WANG Qin, TANG Zhaoxin, YANG Qingqing

2022, 42(6):  642-649.  DOI: 10.3969/j.issn.1000-1565.2022.06.012

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In order to accurately determine rhizosphere soil extracellular enzyme activities, five key enzymes related to the C, N, and P circulation including β-glucosidase(βG), cellobiohydrolase(CBH), β-N-acetylglucosaminidase(NAG), leucine aminopeptidase(LAP), and alkaline phosphatase(AKP)were optimized by fluorescence method. The results showed that the ratio of water to soil should be higher than 300∶1(mL∶g)when using a highly sensitive microplate reader such as Cytation5. The optimal parameters for determining the activities of five extracellular enzymes were as follows: AKP and βG were used with ultrapure water as buffer solution, the soil was mixed with an XH-C vortex mixer(60 W)for 5 min, and incubated at 25 ℃ for 4 h; CBH was used with sodium acetate(0.2 mol/L, pH 5.8)as buffer solution, the soil was mixed with an XH-C vortex mixer(60 W)for 5 min, and incubated at 25 ℃ for 2 h; NAG was used with sodium acetate(0.2 mol/L, pH 5.8)as buffer solution in a shaker(150 r/min)for 60 min, and incubated at 25 ℃ for 3 h; LAP was used with ultrapure water as buffer solution in a shaker(150 r/min)for 60 min, and incubated at 25 ℃ for 1 h. The results of this study can provide a basis for improving the accurate determination of soil extracellular enzyme activities by fluorescence method.

Breeding of fine strains of Auricularia auriculata based on MPMS method

YIN Xianda, LIU Min, YANG Chaoshang, ZHANG Tiejun, WANG Qian

2022, 42(6):  650-656.  DOI: 10.3969/j.issn.1000-1565.2022.06.013

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In order to obtain high-yield and high-quality Auricularia auricula strains, using A. auricula strain Aa66 as the starting strain, multifunction plasma mutagenesis system(MPMS)was used to carry out mulation breeding experiments, and a total of 315 mutant strains were obtained. The antagonism experiment and re-screening were used to determine the growth rate, enzyme activity and biological efficiency of the strain. Through the preliminary screening antagonistic experiment and the determination of strain growth rate,growth trend,enzyme activity and biological efficiency by re-screening, a total of 8 excellent mutagenic strains were obtained, and the inter-simple sequence repeat(ISSR)technology was used to amplify polymorphic molecular markers. They were determined to be significantly different from the starting strain Aa66 at the molecular level. Among them, the excellent mutant strain Y9 grew with the fastest speed of 4.98 mm/d, which was 0.54 mm/d higher than that of the starting strain Aa66, and the biological efficiency was 115.34%, which was 8.43% higher than that of the starting strain Aa66, indicating that MPMS can be used as a new method for breeding excellent A. auricula strains. - DOI:10.3969/j.issn.1000-1565.2022.06.013基于MPMS方法的黑木耳优良菌株选育尹显达¹,刘敏¹,杨超上¹,张铁军²,王谦¹(1.河北大学 生物工程技术创新中心,河北 保定 071002;2.承德市农业环境保护监测站,河北 承德 067000)摘 要:为获得高产优质的黑木耳菌株,以黑木耳(Auricularia auricula)菌株Aa66为出发菌株,采用多功能等离子体诱变系统(multifunction plasma mutagenesis system,MPMS)进行诱变育种实验,共获得315株诱变菌株.通过初筛拮抗实验及复筛对菌株长速长势、酶活及生物学效率的测定,共得到8株优良诱变菌株,且使用简单重复序列区间扩增多态性分子标记技术(inter-simple sequence repeat,ISSR)在分子水平上确定其均与出发菌株Aa66有明显差异,其中优良诱变菌株Y9生长速度最快,为4.98 mm/d,较出发菌株Aa66提高0.54 mm/d,生物学效率为115.34%,较出发菌株Aa66提高8.43%,表明MPMS可以作为一种选育优良黑木耳菌株的新方法.关键词:黑木耳;多功能等离子体诱变系统(MPMS);诱变;分子标记中图分类号:S646.6 文献标志码:A 文章编号:1000-1565(2022)06-0650-07Breeding of fine strains of Auricularia auriculata based on MPMS methodYIN Xianda¹, LIU Min¹, YANG Chaoshang¹, ZHANG Tiejun², WANG Qian¹(1.Bioengineering Technology Innovation Center, Hebei University, Baoding 071002, China; 2.Chengde City Agricultural Environmental Protection Monitoring Station, Chengde 067000, China)Abstract: In order to obtain high-yield and high-quality Auricularia auricula strains, using A. auricula strain Aa66 as the starting strain, multifunction plasma mutagenesis system(MPMS)was used to carry out mulation breeding experiments, and a total of 315 mutant strains were obtained. The antagonism experiment and re-screening were used to determine the growth rate, enzyme activity and biological efficiency of the strain. Through the preliminary screening antagonistic experiment and the determination of strain growth rate,growth trend,enzyme activity and biological efficiency by re-screening, a total of 8 excellent mutagenic strains were obtained, and the inter-simple sequence repeat(ISSR)technology was used to amplify polymorphic molecular markers. They were determined to be significantly different from the starting strain Aa66 at the molecular level. Among them, the excellent mutant strain Y9 grew with the fastest speed of 4.98 mm/d, which was 0.54 mm/d higher than that of the starting strain Aa66, and the biological efficiency was 115.34%, which was 8.43% higher than that of the starting strain Aa66, indicating that MPMS can be used as a new method for breeding excellent A. auricula strains. - 收稿日期:2022-04-28 基金项目:河北省现代农业食用菌产业技术体系创新团队(第2期)(HBCT2018050204) 第一作者:尹显达(1996—),男,河北廊坊人,河北大学在读硕士研究生,主要从事大型真菌研究.E-mail:yin19965@sina.com 通信作者:王谦(1962—),男,北京人,河北大学研究员,主要从事食药用真菌研究与开发工作.E-mail:wq6203_cn@126.com第6期尹显达等:基于MPMS方法的黑木耳优良菌株选育

Research on localization of magnetic field based on neural network

WANG Huaying, SUN Haijun, ZHANG Lei, WANG Xue, HUANG Yanbin, GUO Haijun

2022, 42(6):  657-664.  DOI: 10.3969/j.issn.1000-1565.2022.06.014

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The ubiquitous nature of magnetic fields makes magnetic field localization widely used in target localization and condition detection. However, in the environment containing complex ferromagnetic mass, the variation of the magnetic field signal can lead to problems such as degradation of localization accuracy or even failure to localize. To address these problems, a method combining magnetic field localization with BP neural network is proposed, and experimental verified. The results show that the BP neural network-based magnetic field localization method can be used for the localization of complex environments containing ferromagnetic materials. The localization accuracy is related to the movement step of the magnetic source, the number of magnetic field sensors and the electronic noise of the sensors during data acquisition. The smaller the movement step, the more the sensors and the smaller the electronic- DOI:10.3969/j.issn.1000-1565.2022.06.014基于神经网络的磁场定位技术王华英^{1, 2, 3},孙海军¹,张雷^1,2,3,王学^1,2,3,黄艳宾^1,2,3,郭海军^1,2,3(1.河北工程大学 数理科学与工程学院,河北 邯郸 056038;2.河北省计算光学成像与光电检测技术创新中心,河北 邯郸 056038;3.河北省计算光学成像与智能感测国际联合研究中心,河北 邯郸 056038)摘 要:由于磁场无处不在的特点,使得磁场定位广泛应用于目标定位和状态检测中,然而在含有复杂铁磁质环境下,磁场信号的变化会导致定位精度下降甚至不能定位等问题.针对上述问题,提出了一种将磁场定位与BP神经网络相结合的方法,并进行了实验验证. 结果表明,基于BP神经网络的磁场定位方法可用于含有铁磁质的复杂环境定位. 定位精度与数据采集时磁源的移动步长、磁场传感器数量及传感器电子噪声有关,移动步长越小,传感器数量越多,电子噪声越小,定位精度越高.关键词:磁场;传感器;定位;BP神经网络;定位精度中图分类号:TP394.1 文献标志码:A 文章编号:1000-1565(2022)06-0657-08Research on localization of magnetic field based on neural networkWANG Huaying^1,2,3, SUN Haijun¹, ZHANG Lei^1,2,3, WANG Xue^1,2,3, HUANG Yanbin^1,2,3, GUO Haijun^1,2,3(1. College of Mathematical Science and Engineering, Hebei University of Engineering, Handan 056038, China; 2. Hebei Computational Optical Imaging and Photoelectric Detection Technology Innovation Center, Handan 056038, China; 3. Hebei International Joint Research Center for Computational Optical Imaging and Intelligent Sensing, Handan 056038, China)Abstract: The ubiquitous nature of magnetic fields makes magnetic field localization widely used in target localization and condition detection. However, in the environment containing complex ferromagnetic mass, the variation of the magnetic field signal can lead to problems such as degradation of localization accuracy or even failure to localize. To address these problems, a method combining magnetic field localization with BP neural network is proposed, and experimental verified. The results show that the BP neural network-based magnetic field localization method can be used for the localization of complex environments containing ferromagnetic materials. The localization accuracy is related to the movement step of the magnetic source, the number of magnetic field sensors and the electronic noise of the sensors during data acquisition. The smaller the movement step, the more the sensors and the smaller the electronic- 收稿日期:2022-03-28 基金项目:国家自然科学基金资助项目(62175059);河北省创新能力提升计划资助项目(20540302D);邯郸市科学技术与发展计划项目(21422111246;19422031008-4) 第一作者:王华英(1963—),女,河北涉县人,河北工程大学教授,博士,主要从事机器视觉方面研究.E-mail: pbxsyingzi@126.com 通信作者:郭海军(1967—),男,河北广平人,河北工程大学实验师,主要从事机器视觉方面研究.E-mail: ghj6028039@163.com第6期王华英等:基于神经网络的磁场定位技术noise, the higher the localization accuracy.

Intelligent identification of damage defects in quartz fiber composites using terahertz technique based on convolutional neural network

LI Tao, XUE Gang, HUO Zixiang, WANG Baomin, LI Xiaoling, YANG Zhaonan

2022, 42(6):  665-672.  DOI: 10.3969/j.issn.1000-1565.2022.06.015

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In this paper, terahertz time-domain spectroscopy was used to detect the delamination defects in quartz fiber reinforced polymer(QFRP), and a one-dimensional convolutional neural network was built to realize the accurate identification of damage defects at different positions and depths, and the accuracy of the verification results was more than 90%. The defect detection image of the composite constructed according to the recognition results has high definition and contrast, which was consistent with the actual terahertz image. Terahertz technology combined with convolutional neural network can realize the intelligent recognition of non-polar materials.